DOF56_ARATH
ID DOF56_ARATH Reviewed; 372 AA.
AC Q9FM03; Q0WPR9; Q5HZ57;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 09-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Dof zinc finger protein DOF5.6 {ECO:0000303|PubMed:12475498};
DE Short=AtDOF5.6 {ECO:0000303|PubMed:12475498};
DE AltName: Full=Protein HIGH CAMBIAL ACTIVITY 2 {ECO:0000303|PubMed:19915089};
DE AltName: Full=Protein PHLOEM EARLY DOF HCA2 {ECO:0000303|PubMed:30626969};
GN Name=DOF5.6 {ECO:0000303|PubMed:12475498};
GN Synonyms=HCA2 {ECO:0000303|PubMed:19915089};
GN OrderedLocusNames=At5g62940 {ECO:0000312|Araport:AT5G62940};
GN ORFNames=MQB2.26 {ECO:0000312|EMBL:BAB10863.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12475498; DOI=10.1016/s1360-1385(02)02362-2;
RA Yanagisawa S.;
RT "The Dof family of plant transcription factors.";
RL Trends Plant Sci. 7:555-560(2002).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=19915089; DOI=10.1105/tpc.108.064139;
RA Guo Y., Qin G., Gu H., Qu L.-J.;
RT "Dof5.6/HCA2, a Dof transcription factor gene, regulates interfascicular
RT cambium formation and vascular tissue development in Arabidopsis.";
RL Plant Cell 21:3518-3534(2009).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=30626969; DOI=10.1038/s41586-018-0839-y;
RA Miyashima S., Roszak P., Sevilem I., Toyokura K., Blob B., Heo J.-O.,
RA Mellor N., Help-Rinta-Rahko H., Otero S., Smet W., Boekschoten M.,
RA Hooiveld G., Hashimoto K., Smetana O., Siligato R., Wallner E.-S.,
RA Maehoenen A.P., Kondo Y., Melnyk C.W., Greb T., Nakajima K., Sozzani R.,
RA Bishopp A., De Rybel B., Helariutta Y.;
RT "Mobile PEAR transcription factors integrate positional cues to prime
RT cambial growth.";
RL Nature 565:490-494(2019).
CC -!- FUNCTION: Transcription factor that binds specifically to a 5'-AA[AG]G-
CC 3' consensus core sequence (By similarity). Promotes expression
CC (PubMed:19915089). The PEAR proteins (e.g. DOF2.4, DOF5.1, DOF3.2,
CC DOF1.1, DOF5.6 and DOF5.3) activate gene expression that promotes
CC radial growth of protophloem sieve elements (PubMed:30626969). Involved
CC in the regulation of interfascicular cambium formation and vascular
CC tissue development, particularly at a very early stage during
CC inflorescence stem development; promotes both cambium activity and
CC phloem specification, but prevents xylem specification
CC (PubMed:19915089). {ECO:0000250|UniProtKB:Q9M2U1,
CC ECO:0000269|PubMed:19915089, ECO:0000269|PubMed:30626969}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00071,
CC ECO:0000269|PubMed:19915089}.
CC -!- TISSUE SPECIFICITY: The PEAR proteins (e.g. DOF2.4, DOF5.1, DOF3.2,
CC DOF1.1, DOF5.6 and DOF5.3) form a short-range concentration gradient
CC that peaks at protophloem sieve elements (PSE) (PubMed:30626969).
CC Preferentially expressed in the vasculature of all organs, including
CC seedlings, roots, stems, buds, leaves, flowers and siliques, and
CC particularly in the cambium, phloem and interfascicular parenchyma
CC cells of inflorescence stems (PubMed:19915089).
CC {ECO:0000269|PubMed:19915089, ECO:0000269|PubMed:30626969}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the vascular tissues and pericycle of
CC primary roots. Detected in the vasculature of the cotyledons, rosette
CC leaves and cauline leaves. In flowers, localized in vasculature of
CC petals, stigma, and stamen filaments, and, to a lower extent, in
CC anthers and carpels. In inflorescence stems, accumulates in the
CC vasculature, particularly in cambium, phloem, and interfascicular
CC parenchyma cells. {ECO:0000269|PubMed:19915089}.
CC -!- DISRUPTION PHENOTYPE: Disruption of interfascicular cambium formation
CC and development in inflorescence stems. {ECO:0000269|PubMed:19915089}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10863.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB009053; BAB10863.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97676.1; -; Genomic_DNA.
DR EMBL; BT020467; AAW38968.1; -; mRNA.
DR EMBL; BT020576; AAW78595.1; -; mRNA.
DR EMBL; AK228992; BAF00880.1; -; mRNA.
DR RefSeq; NP_201099.1; NM_125688.3.
DR AlphaFoldDB; Q9FM03; -.
DR STRING; 3702.AT5G62940.1; -.
DR PaxDb; Q9FM03; -.
DR PRIDE; Q9FM03; -.
DR ProteomicsDB; 222127; -.
DR EnsemblPlants; AT5G62940.1; AT5G62940.1; AT5G62940.
DR GeneID; 836414; -.
DR Gramene; AT5G62940.1; AT5G62940.1; AT5G62940.
DR KEGG; ath:AT5G62940; -.
DR Araport; AT5G62940; -.
DR TAIR; locus:2170753; AT5G62940.
DR eggNOG; ENOG502QUGD; Eukaryota.
DR HOGENOM; CLU_036438_2_2_1; -.
DR InParanoid; Q9FM03; -.
DR OMA; LEWQQDQ; -.
DR OrthoDB; 1191225at2759; -.
DR PhylomeDB; Q9FM03; -.
DR PRO; PR:Q9FM03; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FM03; baseline and differential.
DR Genevisible; Q9FM03; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010087; P:phloem or xylem histogenesis; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0010067; P:procambium histogenesis; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0090057; P:root radial pattern formation; IGI:TAIR.
DR InterPro; IPR045174; Dof.
DR InterPro; IPR003851; Znf_Dof.
DR PANTHER; PTHR31992; PTHR31992; 1.
DR Pfam; PF02701; zf-Dof; 1.
DR PROSITE; PS01361; ZF_DOF_1; 1.
DR PROSITE; PS50884; ZF_DOF_2; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..372
FT /note="Dof zinc finger protein DOF5.6"
FT /id="PRO_0000074296"
FT ZN_FING 73..127
FT /note="Dof-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT REGION 117..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 75
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 78
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 100
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 103
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT CONFLICT 309
FT /note="K -> R (in Ref. 4; BAF00880)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 372 AA; 39801 MW; 5F17251324E04E6E CRC64;
MGLTSLQVCM DSDWLQESES SGGSMLDSST NSPSAADILA ACSTRPQASA VAVAAAALMD
GGRRLRPPHD HPQKCPRCES THTKFCYYNN YSLSQPRYFC KTCRRYWTKG GTLRNIPVGG
GCRKNKKPSS SNSSSSTSSG KKPSNIVTAN TSDLMALAHS HQNYQHSPLG FSHFGGMMGS
YSTPEHGNVG FLESKYGGLL SQSPRPIDFL DSKFDLMGVN NDNLVMVNHG SNGDHHHHHN
HHMGLNHGVG LNNNNNNGGF NGISTGGNGN GGGLMDISTC QRLMLSNYDH HHYNHQEDHQ
RVATIMDVKP NPKLLSLDWQ QDQCYSNGGG SGGAGKSDGG GYGNGGYING LGSSWNGLMN
GYGTSTKTNS LV
