DOG1_ARATH
ID DOG1_ARATH Reviewed; 291 AA.
AC A0SVK0; Q0WQP5; Q9FJ53;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Protein DELAY OF GERMINATION 1 {ECO:0000303|PubMed:17065317};
DE AltName: Full=GLUCOSE SENSING QTL 5 {ECO:0000303|PubMed:18410483};
GN Name=DOG1 {ECO:0000303|PubMed:17065317};
GN Synonyms=GSQ5 {ECO:0000303|PubMed:18410483};
GN OrderedLocusNames=At5g45830 {ECO:0000312|Araport:AT5G45830};
GN ORFNames=K15I22.3 {ECO:0000312|EMBL:BAB09311.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:ABK81210.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, GENE
RP FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP ALTERNATIVE SPLICING.
RC STRAIN=cv. Columbia, cv. Cvi-1, cv. Kon, cv. Landsberg erecta, and cv. Sha;
RX PubMed=17065317; DOI=10.1073/pnas.0607877103;
RA Bentsink L., Jowett J., Hanhart C.J., Koornneef M.;
RT "Cloning of DOG1, a quantitative trait locus controlling seed dormancy in
RT Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:17042-17047(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE POLYADENYLATION,
RP FUNCTION, DISRUPTION PHENOTYPE (ISOFORM 1), IDENTIFICATION BY MASS
RP SPECTROMETRY (ISOFORM 2), DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RX PubMed=26620523; DOI=10.1104/pp.15.01483;
RA Cyrek M., Fedak H., Ciesielski A., Guo Y., Sliwa A., Brzezniak L.,
RA Krzyczmonik K., Pietras Z., Kaczanowski S., Liu F., Swiezewski S.;
RT "Seed dormancy in Arabidopsis is controlled by alternative polyadenylation
RT of DOG1.";
RL Plant Physiol. 170:947-955(2016).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-291.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION BY HISTONE MONOUBIQUITINATION.
RX PubMed=17329563; DOI=10.1105/tpc.106.049221;
RA Liu Y., Koornneef M., Soppe W.J.J.;
RT "The absence of histone H2B monoubiquitination in the Arabidopsis hub1
RT (rdo4) mutant reveals a role for chromatin remodeling in seed dormancy.";
RL Plant Cell 19:433-444(2007).
RN [7]
RP FUNCTION, INDUCTION BY GLUCOSE, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, cv. Cvi-1, and cv. Landsberg erecta;
RX PubMed=18410483; DOI=10.1111/j.1365-313x.2008.03515.x;
RA Teng S., Rognoni S., Bentsink L., Smeekens S.;
RT "The Arabidopsis GSQ5/DOG1 Cvi allele is induced by the ABA-mediated sugar
RT signalling pathway, and enhances sugar sensitivity by stimulating ABI4
RT expression.";
RL Plant J. 55:372-381(2008).
RN [8]
RP INDUCTION.
RX PubMed=21569772; DOI=10.1016/j.febslet.2011.04.077;
RA Mortensen S.A., Soenderkaer M., Lynggaard C., Grasser M., Nielsen K.L.,
RA Grasser K.D.;
RT "Reduced expression of the DOG1 gene in Arabidopsis mutant seeds lacking
RT the transcript elongation factor TFIIS.";
RL FEBS Lett. 585:1929-1933(2011).
RN [9]
RP INDUCTION BY COLD.
RC STRAIN=cv. Columbia;
RX PubMed=21803937; DOI=10.1105/tpc.111.087643;
RA Kendall S.L., Hellwege A., Marriot P., Whalley C., Graham I.A.,
RA Penfield S.;
RT "Induction of dormancy in Arabidopsis summer annuals requires parallel
RT regulation of DOG1 and hormone metabolism by low temperature and CBF
RT transcription factors.";
RL Plant Cell 23:2568-2580(2011).
RN [10]
RP FUNCTION, DEVELOPMENTAL STAGE, INDUCTION BY COLD, PROTEIN MODIFICATION,
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=22829147; DOI=10.1105/tpc.112.100214;
RA Nakabayashi K., Bartsch M., Xiang Y., Miatton E., Pellengahr S., Yano R.,
RA Seo M., Soppe W.J.;
RT "The time required for dormancy release in Arabidopsis is determined by
RT DELAY OF GERMINATION1 protein levels in freshly harvested seeds.";
RL Plant Cell 24:2826-2838(2012).
RN [11]
RP FUNCTION.
RX PubMed=25114251; DOI=10.1073/pnas.1403851111;
RA Graeber K., Linkies A., Steinbrecher T., Mummenhoff K., Tarkowska D.,
RA Tureckova V., Ignatz M., Sperber K., Voegele A., de Jong H., Urbanova T.,
RA Strnad M., Leubner-Metzger G.;
RT "DELAY OF GERMINATION 1 mediates a conserved coat-dormancy mechanism for
RT the temperature- and gibberellin-dependent control of seed germination.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:E3571-E3580(2014).
RN [12]
RP SPLICING REGULATION.
RX PubMed=25568310; DOI=10.15252/embj.201489478;
RA Dolata J., Guo Y., Kolowerzo A., Smolinski D., Brzyzek G., Jarmolowski A.,
RA Swiezewski S.;
RT "NTR1 is required for transcription elongation checkpoints at alternative
RT exons in Arabidopsis.";
RL EMBO J. 34:544-558(2015).
RN [13]
RP INDUCTION BY LDL1 AND LDL2.
RX PubMed=25852712; DOI=10.3389/fpls.2015.00159;
RA Zhao M., Yang S., Liu X., Wu K.;
RT "Arabidopsis histone demethylases LDL1 and LDL2 control primary seed
RT dormancy by regulating DELAY OF GERMINATION 1 and ABA signaling-related
RT genes.";
RL Front. Plant Sci. 6:159-159(2015).
RN [14]
RP ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-15, AND
RP SUBUNIT.
RX PubMed=26684465; DOI=10.1371/journal.pgen.1005737;
RA Nakabayashi K., Bartsch M., Ding J., Soppe W.J.;
RT "Seed dormancy in Arabidopsis requires self-binding ability of DOG1 protein
RT and the presence of multiple isoforms generated by alternative splicing.";
RL PLoS Genet. 11:E1005737-E1005737(2015).
RN [15]
RP FUNCTION.
RX PubMed=26729600; DOI=10.1111/tpj.13118;
RA Dekkers B.J., He H., Hanson J., Willems L.A., Jamar D.C., Cueff G.,
RA Rajjou L., Hilhorst H.W., Bentsink L.;
RT "The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID
RT INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during
RT Arabidopsis seed development.";
RL Plant J. 85:451-465(2016).
RN [16]
RP FUNCTION.
RX PubMed=27035986; DOI=10.1073/pnas.1600558113;
RA Huo H., Wei S., Bradford K.J.;
RT "DELAY OF GERMINATION1 (DOG1) regulates both seed dormancy and flowering
RT time through microRNA pathways.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:E2199-E2206(2016).
CC -!- FUNCTION: Required for the induction of seed dormancy (PubMed:17065317,
CC PubMed:26620523, PubMed:22829147). The level of DOG1 protein in freshly
CC harvested seeds determines the level of seed dormancy
CC (PubMed:22829147). Determines the temperature window for germination by
CC regulating the expression of micropylar endosperm-weakening genes
CC through temperature control of the gibberellins metabolism
CC (PubMed:25114251). Regulates seed dormancy and flowering time through
CC an influence on levels of microRNAs miR156 and miR172
CC (PubMed:27035986). Regulator of seed maturation interfering with
CC abscisic acid signaling components and activating ABI5
CC (PubMed:26729600). In cv. Cvi-1, enhances glucose induction of ABI4
CC (PubMed:18410483). {ECO:0000269|PubMed:17065317,
CC ECO:0000269|PubMed:18410483, ECO:0000269|PubMed:22829147,
CC ECO:0000269|PubMed:25114251, ECO:0000269|PubMed:26620523,
CC ECO:0000269|PubMed:26729600, ECO:0000269|PubMed:27035986}.
CC -!- SUBUNIT: Homodimer (PubMed:26684465). Self-binding does not influence
CC protein accumulation but is required for full function
CC (PubMed:26684465). Single isoforms are functional, but the presence of
CC additional isoforms is required to prevent protein degradation
CC (PubMed:26684465). {ECO:0000269|PubMed:26684465}.
CC -!- INTERACTION:
CC A0SVK0; Q9FLI3: AHG1; NbExp=3; IntAct=EBI-25512274, EBI-2363348;
CC A0SVK0; Q9LNW3: AIP1; NbExp=5; IntAct=EBI-25512274, EBI-1573499;
CC A0SVK0; Q9ZW21: At2g29380; NbExp=5; IntAct=EBI-25512274, EBI-4441103;
CC A0SVK0; A0SVK0: DOG1; NbExp=3; IntAct=EBI-25512274, EBI-25512274;
CC A0SVK0; O82132: DREB2A; NbExp=3; IntAct=EBI-25512274, EBI-1786840;
CC A0SVK0; P49598: PP2CA; NbExp=3; IntAct=EBI-25512274, EBI-1764934;
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Nucleus
CC {ECO:0000269|PubMed:22829147, ECO:0000269|PubMed:26620523,
CC ECO:0000269|PubMed:26684465}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Nucleus
CC {ECO:0000269|PubMed:26620523}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=A number of isoforms are produced. {ECO:0000305};
CC Name=1;
CC IsoId=A0SVK0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A0SVK0-2; Sequence=VSP_058562, VSP_058563;
CC -!- TISSUE SPECIFICITY: Detected only in seeds (PubMed:17065317). Expressed
CC mainly in vascular tissues of the cotyledon, hypocotyl and radicle of
CC the embryo. {ECO:0000269|PubMed:17065317, ECO:0000269|PubMed:22829147}.
CC -!- DEVELOPMENTAL STAGE: Isoform 1 and isoform 2: Transcription starts at
CC the beginning of seed maturation 9 days after pollination and reaches
CC its highest level during the last phases of seed development
CC (PubMed:26620523, PubMed:17065317, PubMed:22829147). Isoform 1: Upon
CC imbibition the transcripts rapidly disappear in both dormant and after-
CC ripened seeds, but the level of protein is hardly affected
CC (PubMed:17065317, PubMed:22829147). Isoform 2: Transcription starts at
CC the beginning of seed maturation 9 days after pollination and reaches
CC its highest level during the last phases of seed development
CC (PubMed:26620523). {ECO:0000269|PubMed:17065317,
CC ECO:0000269|PubMed:22829147, ECO:0000269|PubMed:26620523}.
CC -!- INDUCTION: Up-regulation by glucose of the Cvi-1 allele, but not of the
CC Columbia and Landsberg erecta alleles (PubMed:18410483). May be up-
CC regulated by the transcript elongation factor TFIIS (PubMed:21569772).
CC Up-regulated by cold (PubMed:21803937, PubMed:22829147). Up-regulated
CC by histone monoubiquitination through HUB1 (PubMed:17329563). Down-
CC regulated by the histone demethylases LDL1 and LDL2 (PubMed:25852712).
CC {ECO:0000269|PubMed:17329563, ECO:0000269|PubMed:18410483,
CC ECO:0000269|PubMed:21803937, ECO:0000269|PubMed:22829147,
CC ECO:0000269|PubMed:25852712, ECO:0000305|PubMed:21569772}.
CC -!- PTM: A shift in isoelectric focusing of the protein occurs during
CC after-ripening, probably leading to its loss of function.
CC {ECO:0000269|PubMed:22829147}.
CC -!- DISRUPTION PHENOTYPE: Loss of seed dormancy, but no effect on sugar
CC sensitivity (in cv. Columbia) (PubMed:17065317, PubMed:18410483). A T-
CC DNA insertion mutant lacking the long version of the DOG1 transcript
CC (isoform 1) but expressing isoform 2 exhibits stronger seed dormancy
CC (PubMed:26620523). {ECO:0000269|PubMed:17065317,
CC ECO:0000269|PubMed:18410483, ECO:0000269|PubMed:26620523}.
CC -!- MISCELLANEOUS: The Columbia and Landsberg erecta alleles have weak
CC dormancy phenotypes, whereas the Cvi-1 allele shows a strong dormancy
CC phenotype (PubMed:17065317). The Cvi-1 allele shows a sugar-
CC supersensitive phenotype, whereas the Columbia and Landsberg erecta
CC alleles don't have a significant effect on sugar sensitivity
CC (PubMed:18410483). {ECO:0000269|PubMed:17065317,
CC ECO:0000269|PubMed:18410483}.
CC -!- MISCELLANEOUS: Transgenic mutant seeds expressing single DOG1
CC transcript variants lack dormancy and do not accumulate DOG1 protein.
CC Simultaneous expression of two or more DOG1 transcript variants
CC encoding different isoforms, however, leads to the accumulation of DOG1
CC protein and increased seed dormancy (PubMed:26684465). The spliceosome
CC disassembly factor STIP1/NTR1 is required for proper transcript levels
CC and splicing of DOG1 (PubMed:25568310). {ECO:0000269|PubMed:25568310,
CC ECO:0000269|PubMed:26684465}.
CC -!- MISCELLANEOUS: [Isoform 2]: The use of a proximal polyadenylation site
CC leads to the production of a short DOG1 mRNA that is translated in
CC vivo, producing an isoform that is sufficient for seed dormancy
CC establishment. {ECO:0000269|PubMed:26620523}.
CC -!- MISCELLANEOUS: [Isoform 2]: Produced by alternative polyadenylation.
CC {ECO:0000269|PubMed:26620523}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09311.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EF028469; ABK81210.1; -; Genomic_DNA.
DR EMBL; EF028472; ABK81213.1; -; Genomic_DNA.
DR EMBL; AB016870; BAB09311.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; ANM68819.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68820.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68821.1; -; Genomic_DNA.
DR EMBL; AK228646; BAF00554.1; -; mRNA.
DR RefSeq; NP_001330541.1; NM_001344645.1. [A0SVK0-2]
DR RefSeq; NP_001330542.1; NM_001344642.1. [A0SVK0-2]
DR RefSeq; NP_199395.2; NM_123951.3. [A0SVK0-1]
DR AlphaFoldDB; A0SVK0; -.
DR SMR; A0SVK0; -.
DR IntAct; A0SVK0; 5.
DR STRING; 3702.AT5G45830.1; -.
DR PaxDb; A0SVK0; -.
DR PRIDE; A0SVK0; -.
DR ProteomicsDB; 222130; -. [A0SVK0-1]
DR EnsemblPlants; AT5G45830.2; AT5G45830.2; AT5G45830. [A0SVK0-2]
DR EnsemblPlants; AT5G45830.3; AT5G45830.3; AT5G45830. [A0SVK0-1]
DR EnsemblPlants; AT5G45830.6; AT5G45830.6; AT5G45830. [A0SVK0-2]
DR GeneID; 834623; -.
DR Gramene; AT5G45830.2; AT5G45830.2; AT5G45830. [A0SVK0-2]
DR Gramene; AT5G45830.3; AT5G45830.3; AT5G45830. [A0SVK0-1]
DR Gramene; AT5G45830.6; AT5G45830.6; AT5G45830. [A0SVK0-2]
DR KEGG; ath:AT5G45830; -.
DR Araport; AT5G45830; -.
DR TAIR; locus:2152390; AT5G45830.
DR eggNOG; ENOG502QW7X; Eukaryota.
DR HOGENOM; CLU_024782_2_2_1; -.
DR InParanoid; A0SVK0; -.
DR OMA; HSGHMAD; -.
DR PhylomeDB; A0SVK0; -.
DR PRO; PR:A0SVK0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; A0SVK0; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IMP:TAIR.
DR GO; GO:2000033; P:regulation of seed dormancy process; IMP:TAIR.
DR GO; GO:0010162; P:seed dormancy process; IMP:TAIR.
DR GO; GO:0010182; P:sugar mediated signaling pathway; IMP:TAIR.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR025422; TGA_domain.
DR Pfam; PF14144; DOG1; 1.
DR PROSITE; PS51806; DOG1; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Germination; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..291
FT /note="Protein DELAY OF GERMINATION 1"
FT /id="PRO_0000437685"
FT DOMAIN 8..258
FT /note="DOG1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01147"
FT VAR_SEQ 268..276
FT /note="VIFDACTTV -> GNAGGEEGK (in isoform 2)"
FT /id="VSP_058562"
FT VAR_SEQ 277..291
FT /note="Missing (in isoform 2)"
FT /id="VSP_058563"
FT MUTAGEN 15
FT /note="Y->A: Strongly reduced self-binding."
FT /evidence="ECO:0000269|PubMed:26684465"
SQ SEQUENCE 291 AA; 32470 MW; 58BC5E3F940889ED CRC64;
MGSSSKNIEQ AQDSYLEWMS LQSQRIPELK QLLAQRRSHG DEDNDNKLRK LTGKIIGDFK
NYAAKRADLA HRCSSNYYAP TWNSPLENAL IWMGGCRPSS FFRLVYALCG SQTEIRVTQF
LRNIDGYESS GGGGGASLSD LSAEQLAKIN VLHVKIIDEE EKMTKKVSSL QEDAADIPIA
TVAYEMENVG EPNVVVDQAL DKQEEAMARL LVEADNLRVD TLAKILGILS PVQGADFLLA
GKKLHLSMHE WGTMRDRRRR DCMVDTEVIF DACTTVNSGP RPTETTNNER N
