DON1_YEAST
ID DON1_YEAST Reviewed; 365 AA.
AC Q05610; D6VSQ5; Q66RE0;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Donuts protein 1;
GN Name=DON1; OrderedLocusNames=YDR273W; ORFNames=D9954.4;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=10899120; DOI=10.1093/emboj/19.14.3657;
RA Knop M., Strasser K.;
RT "Role of the spindle pole body of yeast in mediating assembly of the
RT prospore membrane during meiosis.";
RL EMBO J. 19:3657-3667(2000).
RN [5]
RP COMPOSITION OF A SPB COMPLEX.
RX PubMed=11742972; DOI=10.1093/emboj/20.24.6946;
RA Moreno-Borchart A.C., Strasser K., Finkbeiner M.G., Shevchenko A.,
RA Shevchenko A., Knop M.;
RT "Prospore membrane formation linked to the leading edge protein (LEP) coat
RT assembly.";
RL EMBO J. 20:6946-6957(2001).
RN [6]
RP RECRUITMENT BY ADY3.
RX PubMed=11973299; DOI=10.1093/genetics/160.4.1439;
RA Nickas M.E., Neiman A.M.;
RT "Ady3p links spindle pole body function to spore wall synthesis in
RT Saccharomyces cerevisiae.";
RL Genetics 160:1439-1450(2002).
CC -!- FUNCTION: Involved in the pathway that organizes the prospore membrane
CC (PSM) during sporulation.
CC -!- SUBUNIT: May interact directly with ADY3. Probable component of a
CC spindle pole body (SPB) complex composed of ADY3, SSP1, DON1, MPC54,
CC SPO21/MPC70, NUD1 and CNM67.
CC -!- SUBCELLULAR LOCATION: Prospore membrane {ECO:0000269|PubMed:10899120}.
CC Note=Towards the end of meiosis I, or early stages of meiosis II, it
CC forms circular structures above the meiotic plaque. As soon as an
CC assembled prospore membrane is visible during meiosis II, it is
CC detected at the leading edge, which cover the ring-shape opening of the
CC PSMs. Probably recruited to the leading edges by ADY3.
CC -!- DEVELOPMENTAL STAGE: Meiosis-specific. {ECO:0000269|PubMed:10899120}.
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DR EMBL; U51030; AAB64449.1; -; Genomic_DNA.
DR EMBL; AY723788; AAU09705.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12115.1; -; Genomic_DNA.
DR PIR; S70131; S70131.
DR RefSeq; NP_010559.3; NM_001180581.3.
DR AlphaFoldDB; Q05610; -.
DR BioGRID; 32327; 41.
DR DIP; DIP-1577N; -.
DR IntAct; Q05610; 9.
DR MINT; Q05610; -.
DR STRING; 4932.YDR273W; -.
DR PaxDb; Q05610; -.
DR PRIDE; Q05610; -.
DR EnsemblFungi; YDR273W_mRNA; YDR273W; YDR273W.
DR GeneID; 851866; -.
DR KEGG; sce:YDR273W; -.
DR SGD; S000002681; DON1.
DR VEuPathDB; FungiDB:YDR273W; -.
DR GeneTree; ENSGT00390000013104; -.
DR HOGENOM; CLU_839786_0_0_1; -.
DR InParanoid; Q05610; -.
DR BioCyc; YEAST:G3O-29840-MON; -.
DR PRO; PR:Q05610; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q05610; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005628; C:prospore membrane; IDA:SGD.
DR GO; GO:0070056; C:prospore membrane leading edge; IDA:SGD.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0031624; F:ubiquitin conjugating enzyme binding; IBA:GO_Central.
DR GO; GO:0030476; P:ascospore wall assembly; IPI:SGD.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd14372; CUE_Cue5p_like; 1.
DR InterPro; IPR003892; CUE.
DR InterPro; IPR041807; Cue5/Don1_CUE.
DR InterPro; IPR009060; UBA-like_sf.
DR Pfam; PF02845; CUE; 1.
DR SMART; SM00546; CUE; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
DR PROSITE; PS51140; CUE; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Meiosis; Membrane; Reference proteome;
KW Sporulation.
FT CHAIN 1..365
FT /note="Donuts protein 1"
FT /id="PRO_0000079977"
FT DOMAIN 82..125
FT /note="CUE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00468"
FT REGION 28..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 215..246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 286..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 224..245
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..314
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 327
FT /note="K -> E (in Ref. 3; AAU09705)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 365 AA; 40162 MW; 6E13542050862709 CRC64;
MGKKNRKGKE NNAAKTSFLK VENIKNTNSG LELPSQDYTN VEEKESSPKT DFPLITKEHV
NTKTDSNILD YPTIGDLVSS VEKLCVLKEL KIAFPEVDDT LIKAILIASQ GVLEPAFNSL
LYYSSPEENT DFALPMKPIS VEDYSKINVS EILQREIFDD IEDEFSGQGI NGSMVISKIE
SELSSLAEHI GNISTPGSNR EVAESTRNVA VAEGHNTILS NEDSILKGKE KGKEEEKEKG
EEKGVNSLKG AAVKVVAKSL KNNRIPVTVK RNEPSNNLFD VLNCDESEEE EEQDVETNTS
NQERKNQGGN TEVPEAQRDS ADRLPAKDDG GYKSAFGTDS CGLFAADAKD EKKQVHPSRQ
ELSFT
