DONS_DROME
ID DONS_DROME Reviewed; 568 AA.
AC Q9VNA8; Q540V6;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Protein downstream neighbor of son homolog;
DE AltName: Full=Protein humpty dumpty;
GN Name=hd; ORFNames=CG2669;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=15854909; DOI=10.1016/j.cub.2005.02.063;
RA Bandura J.L., Beall E.L., Bell M., Silver H.R., Botchan M.R., Calvi B.R.;
RT "humpty dumpty is required for developmental DNA amplification and cell
RT proliferation in Drosophila.";
RL Curr. Biol. 15:755-759(2005).
CC -!- FUNCTION: Essential for DNA amplification in the ovary and required for
CC cell proliferation during development. {ECO:0000269|PubMed:15854909}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15854909}.
CC -!- TISSUE SPECIFICITY: Expression peaks during late G1 and S phase (at
CC protein level). {ECO:0000269|PubMed:15854909}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC {ECO:0000269|PubMed:15854909}.
CC -!- DISRUPTION PHENOTYPE: Flies display a distinct thin-eggshell phenotype.
CC {ECO:0000269|PubMed:15854909}.
CC -!- SIMILARITY: Belongs to the DONSON family. {ECO:0000305}.
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DR EMBL; AE014297; AAF52038.1; -; Genomic_DNA.
DR EMBL; AY121655; AAM51982.1; -; mRNA.
DR RefSeq; NP_001303424.1; NM_001316495.1.
DR RefSeq; NP_649531.1; NM_141274.3.
DR AlphaFoldDB; Q9VNA8; -.
DR SMR; Q9VNA8; -.
DR BioGRID; 65852; 2.
DR STRING; 7227.FBpp0078445; -.
DR PaxDb; Q9VNA8; -.
DR EnsemblMetazoa; FBtr0078802; FBpp0078445; FBgn0086695.
DR EnsemblMetazoa; FBtr0347559; FBpp0312592; FBgn0086695.
DR GeneID; 40642; -.
DR KEGG; dme:Dmel_CG2669; -.
DR CTD; 40642; -.
DR FlyBase; FBgn0086695; hd.
DR VEuPathDB; VectorBase:FBgn0086695; -.
DR eggNOG; KOG4734; Eukaryota.
DR GeneTree; ENSGT00390000000447; -.
DR HOGENOM; CLU_021567_1_0_1; -.
DR InParanoid; Q9VNA8; -.
DR OMA; RCMDPQR; -.
DR OrthoDB; 479613at2759; -.
DR PhylomeDB; Q9VNA8; -.
DR BioGRID-ORCS; 40642; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40642; -.
DR PRO; PR:Q9VNA8; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0086695; Expressed in secondary oocyte and 33 other tissues.
DR ExpressionAtlas; Q9VNA8; baseline and differential.
DR Genevisible; Q9VNA8; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007307; P:eggshell chorion gene amplification; IMP:FlyBase.
DR GO; GO:0033260; P:nuclear DNA replication; IMP:FlyBase.
DR InterPro; IPR024861; Donson.
DR PANTHER; PTHR12972; PTHR12972; 1.
DR PRINTS; PR02064; DONSON.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Developmental protein; Nucleus;
KW Reference proteome.
FT CHAIN 1..568
FT /note="Protein downstream neighbor of son homolog"
FT /id="PRO_0000079978"
FT REGION 28..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 311..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 311..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 568 AA; 63438 MW; A7E29D4C0AFD14B0 CRC64;
MSTEASVASK WTRPDDFIKL QRLKQKKNKL AARVSNNNNR RPRHQVDETD KSKLLEAKLA
QKRKNPFAKS TADAKKLRVD PQLADLEPVV TASSCFVRET PTRPAPAKFV LQKYDPQAFA
KLFQHPQINA EDEDDAELAA RQKHTAHLPV DWSLKTRARF FCPTELPAIQ LKTSQLASGL
TSFVRCMDPQ RTESTLDISD ATRFNQCNYY WQHPHLPWLT LYPRTAKENV GVVVGERERK
ALAEEWDFSF RGLFQLLRAR QCPYFYLCAN TFTVLFRAAG VGGRPESHAL VTPSTRGMRQ
ALRQEGIEFS MPLKSDNSGN AHDNSFNEES TTTSLGPEAG EDAPPPAQED EDDDEDWLES
LGVDERELRR IQSSHARKQQ AAEMREDFSD NSLLLVDGVE CQGFFSYLLN AKSAISTVGR
LAGVPPTLLS PVAFPKATMQ HLVPRSKKVR LDGVDYFSID IKGLILPTFL PSVAELLSET
RQMFSSTLAS SINTLAFSKA TQKLLETPET PQSDAEGEDA AGQVFGEQNL SECGLLPAVV
GSICRTGQHA VGLLERVCYQ RDEGYAWS
