DONS_MOUSE
ID DONS_MOUSE Reviewed; 560 AA.
AC Q9QXP4; Q80V93; Q8BV55;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 24-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Protein downstream neighbor of Son;
DE AltName: Full=Protein 3SG;
GN Name=Donson; Synonyms=ORF60;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-377.
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 232-560.
RC STRAIN=129/Sv;
RX PubMed=10950926; DOI=10.1006/geno.2000.6254;
RA Wynn S.L., Fisher R.A., Pagel C., Price M., Liu Q.Y., Khan I.M., Zammit P.,
RA Dadrah K., Mazrani W., Kessling A., Lee J.S., Buluwela L.;
RT "Organization and conservation of the GART/SON/DONSON locus in mouse and
RT human genomes.";
RL Genomics 68:57-62(2000).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=28630177; DOI=10.1101/gr.219899.116;
RA Evrony G.D., Cordero D.R., Shen J., Partlow J.N., Yu T.W., Rodin R.E.,
RA Hill R.S., Coulter M.E., Lam A.N., Jayaraman D., Gerrelli D., Diaz D.G.,
RA Santos C., Morrison V., Galli A., Tschulena U., Wiemann S., Martel M.J.,
RA Spooner B., Ryu S.C., Elhosary P.C., Richardson J.M., Tierney D.,
RA Robinson C.A., Chibbar R., Diudea D., Folkerth R., Wiebe S.,
RA Barkovich A.J., Mochida G.H., Irvine J., Lemire E.G., Blakley P.,
RA Walsh C.A.;
RT "Integrated genome and transcriptome sequencing identifies a noncoding
RT mutation in the genome replication factor DONSON as the cause of
RT microcephaly-micromelia syndrome.";
RL Genome Res. 27:1323-1335(2017).
CC -!- FUNCTION: Replisome component that maintains genome stability by
CC protecting stalled or damaged replication forks. After the induction of
CC replication stress, required for the stabilization of stalled
CC replication forks, the efficient activation of the intra-S-phase and
CC G/2M cell-cycle checkpoints and the maintenance of genome stability.
CC {ECO:0000250|UniProtKB:Q9NYP3}.
CC -!- SUBUNIT: Component of the replisome complex composed of at least MCM2,
CC MCM7, PCNA and TICRR; interaction at least with PCNA occurs during DNA
CC replication. {ECO:0000250|UniProtKB:Q9NYP3}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NYP3}.
CC Note=Localizes at DNA replication sites.
CC {ECO:0000250|UniProtKB:Q9NYP3}.
CC -!- DEVELOPMENTAL STAGE: At 14.5 dpc, widely expressed, including in
CC neocortex, cerebellum, lung, liver, hindlimb, intestine, as well as
CC forelimb, kidney and spinal cord vertebrae.
CC {ECO:0000269|PubMed:28630177}.
CC -!- DISRUPTION PHENOTYPE: Early embryonic lethality. Heterozygous mice show
CC no overt phenotype. {ECO:0000269|PubMed:28630177}.
CC -!- SIMILARITY: Belongs to the DONSON family. {ECO:0000305}.
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DR EMBL; BC043316; AAH43316.1; -; mRNA.
DR EMBL; AK080299; BAC37869.1; -; mRNA.
DR EMBL; AF193608; AAF23122.1; -; mRNA.
DR CCDS; CCDS28330.1; -.
DR RefSeq; NP_068366.1; NM_021720.1.
DR RefSeq; XP_006523132.1; XM_006523069.3.
DR AlphaFoldDB; Q9QXP4; -.
DR STRING; 10090.ENSMUSP00000023682; -.
DR iPTMnet; Q9QXP4; -.
DR PhosphoSitePlus; Q9QXP4; -.
DR EPD; Q9QXP4; -.
DR MaxQB; Q9QXP4; -.
DR PaxDb; Q9QXP4; -.
DR PRIDE; Q9QXP4; -.
DR ProteomicsDB; 277373; -.
DR Ensembl; ENSMUST00000023682; ENSMUSP00000023682; ENSMUSG00000022960.
DR GeneID; 60364; -.
DR KEGG; mmu:60364; -.
DR UCSC; uc007zyc.1; mouse.
DR CTD; 29980; -.
DR MGI; MGI:1890621; Donson.
DR VEuPathDB; HostDB:ENSMUSG00000022960; -.
DR eggNOG; KOG4734; Eukaryota.
DR GeneTree; ENSGT00390000000447; -.
DR InParanoid; Q9QXP4; -.
DR OMA; RCMDPQR; -.
DR OrthoDB; 479613at2759; -.
DR PhylomeDB; Q9QXP4; -.
DR TreeFam; TF318976; -.
DR BioGRID-ORCS; 60364; 21 hits in 75 CRISPR screens.
DR ChiTaRS; Donson; mouse.
DR PRO; PR:Q9QXP4; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9QXP4; protein.
DR Bgee; ENSMUSG00000022960; Expressed in spermatocyte and 242 other tissues.
DR ExpressionAtlas; Q9QXP4; baseline and differential.
DR Genevisible; Q9QXP4; MM.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005657; C:replication fork; ISS:UniProtKB.
DR GO; GO:0030894; C:replisome; ISS:UniProtKB.
DR GO; GO:0000077; P:DNA damage checkpoint signaling; ISS:UniProtKB.
DR GO; GO:0006260; P:DNA replication; ISS:UniProtKB.
DR GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; ISS:UniProtKB.
DR GO; GO:0033260; P:nuclear DNA replication; IBA:GO_Central.
DR GO; GO:0048478; P:replication fork protection; ISS:UniProtKB.
DR InterPro; IPR024861; Donson.
DR PANTHER; PTHR12972; PTHR12972; 1.
DR PRINTS; PR02064; DONSON.
PE 1: Evidence at protein level;
KW Developmental protein; Nucleus; Reference proteome.
FT CHAIN 1..560
FT /note="Protein downstream neighbor of Son"
FT /id="PRO_0000079980"
FT REGION 1..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..90
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 123..140
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 32
FT /note="A -> D (in Ref. 2; BAC37869)"
FT /evidence="ECO:0000305"
FT CONFLICT 36
FT /note="S -> L (in Ref. 2; BAC37869)"
FT /evidence="ECO:0000305"
FT CONFLICT 72
FT /note="R -> M (in Ref. 2; BAC37869)"
FT /evidence="ECO:0000305"
FT CONFLICT 93
FT /note="L -> V (in Ref. 2; BAC37869)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="Q -> H (in Ref. 2; BAC37869)"
FT /evidence="ECO:0000305"
FT CONFLICT 100
FT /note="S -> W (in Ref. 2; BAC37869)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 560 AA; 61987 MW; 953AE708BE5686BA CRC64;
MAVSVPGYSP SFKRPPETVR LRRKRSRDHG AAVPASLPEP APRRAALAAG LPLRPFPTAG
GRGGAAATIA RRNPFARLDN RPRVSDEASE EPLRGPQGAS GPLLDSNEEN NLLWEDTSSH
ERTGTELSQS QRVSLSESDT WSSDGTELPV DWSIKTRLLF TSSQPFSWAD HLKAQEEAQG
LVQHCRATEV TLPQSIQDPK LSTALRCAFQ QALVYWLHPA FSWLPLFPRI GADRKMAAKT
SPWSADETLQ HALMSDWSVS FTSLYNLLKT KLCPYFYVCS YQFTVLFRAA GLAGSSVITA
LISPTTRGLR EAMRNEGIEF SLPLLEEIGH KKKVRDPSLE SEEEQAVSDE DEEESFSWLE
EIGVQDQIKK PDVISIKLRK EKHEVQMDHR PESVVLVKGL NTFKLLNFLI NCKSLVATSG
AQAGLPPTLL SPIAFRGASM QMLKARSSNV KTQALSGYRD KFSLDITGPV MPHALHSMSM
LLRSSQRGSF SAGLYAHEPT AVFNVGLSLD KELDRKVARE DLANCGLHPK TLEQLSQRPV
LGKSSLRSVE MSDYILSWRS
