DOP1_DROME
ID DOP1_DROME Reviewed; 2599 AA.
AC A1ZBE8; Q8T9G6;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Protein dopey-1 homolog;
GN ORFNames=CG15099;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 425-2599.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-753; THR-1355; SER-1360;
RP SER-1363; SER-1371 AND SER-2525, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: May be involved in protein traffic between late Golgi and
CC early endosomes. {ECO:0000250|UniProtKB:Q03921}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q03921}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q03921}.
CC -!- SIMILARITY: Belongs to the dopey family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL39920.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE013599; AAF57626.1; -; Genomic_DNA.
DR EMBL; AY069775; AAL39920.1; ALT_INIT; mRNA.
DR RefSeq; NP_611381.2; NM_137537.4.
DR AlphaFoldDB; A1ZBE8; -.
DR BioGRID; 62844; 4.
DR IntAct; A1ZBE8; 3.
DR STRING; 7227.FBpp0085810; -.
DR iPTMnet; A1ZBE8; -.
DR PaxDb; A1ZBE8; -.
DR PRIDE; A1ZBE8; -.
DR EnsemblMetazoa; FBtr0086628; FBpp0085810; FBgn0034400.
DR GeneID; 37176; -.
DR KEGG; dme:Dmel_CG15099; -.
DR UCSC; CG15099-RA; d. melanogaster.
DR FlyBase; FBgn0034400; CG15099.
DR VEuPathDB; VectorBase:FBgn0034400; -.
DR eggNOG; KOG3613; Eukaryota.
DR GeneTree; ENSGT00390000016421; -.
DR HOGENOM; CLU_001045_0_0_1; -.
DR InParanoid; A1ZBE8; -.
DR OMA; QFQRNST; -.
DR OrthoDB; 29961at2759; -.
DR PhylomeDB; A1ZBE8; -.
DR BioGRID-ORCS; 37176; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 37176; -.
DR PRO; PR:A1ZBE8; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034400; Expressed in antenna and 26 other tissues.
DR ExpressionAtlas; A1ZBE8; baseline and differential.
DR Genevisible; A1ZBE8; DM.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0006895; P:Golgi to endosome transport; IEA:InterPro.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR040314; DOP1.
DR InterPro; IPR007249; Dopey_N.
DR PANTHER; PTHR14042; PTHR14042; 1.
DR Pfam; PF04118; Dopey_N; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..2599
FT /note="Protein dopey-1 homolog"
FT /id="PRO_0000297953"
FT REGION 532..571
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 595..701
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1240..1316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1347..1368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1409..1442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 623..638
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1240..1254
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1259..1284
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1285..1307
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1409..1424
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 753
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1355
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1360
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1363
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1371
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 2525
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 1690
FT /note="L -> R (in Ref. 3; AAL39920)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2599 AA; 291188 MW; DE58111DBA51CF42 CRC64;
MESADALVEE QKLMAEAKYR TYMTNIDKAL RNFEYSSEWA DLISALGKLS KAISSNTQYQ
VIPRRLKIAK RLAQCMHPAL PSGVHLKALE TYSVIFSKTG PERLATEFIY SAGLFPLLGY
AAMNVRPALL AIYETYFVPL GDKLRPALSG FLNGVLPGYD CGLDHFERIS ALLKQVCNAV
NPMHFYTVLW ESVANNAAIR LPAITYLLEH FNKRLDMQEQ IYIMGHNREI MMSALCVCLN
DSLILVQRNT LEFLLLGFPM HTVLLSEDDL VKLVTNGLNT ILRRDMSLNR RLFSWLLGSE
VAKNSPTYDT LSLDTSNAPL EEEPEPYFVK HSRHILIKAL ITTLRLSLEC APMDLKPYRI
MLSLLDKAEI GSAVLDYVLH DIIRAMYISS GNAEALKSAN LLFATFDPAY IWSYMTNMFE
KACQQANSMQ EKSQAGETSG KYACDVGSGD PCVLEICVLT EFLLETVSLE MYTETTRVYL
PKVFLAITQL LSLHMDHISS NEITASLKLC MKIVSRVQPM ITSPIKLNKL IEQSGGSSSD
EKITMNSASD AGKTEAGAQG NSLEKSKSDS RLNQFAENTL HSADPNDEEL IHRSASNQSV
GRQSPNKKKA KSISRLSELD KDISASDTGQ QSSSDLDTPR SIKKLKAKAK VPFIRSPKKQ
RPKDMVLIQS NTSAEVSDGP SAEEPKSAPP DQTPQFQLDE ESRATQQRGF SILEKCIRQY
EIFFEVYLSR KLLHIESEPK ECSVKVQLQR TTSTIHTSNL FMAEQVLDHE CPVRESQIER
LFNLLRVDIV PRSKQLQRLL NRSLPLPASA SELSSDSEAQ EEKQQSLLID GQTQRSVQQL
AELQLSPSLR GAVKLAATLL VEMSTFPNCN KHVVLDKNEP ELPNWLKVLC LVACFAQSDK
ELQVASITTL FDLISLLRSQ IEHTTSPGVT FVVMLPLLKF GHVSYMEQHT RVFQLVSSIL
WDYLGTAGID PAQIAALLHQ LHSCLESGLV ETVIGNRMQS QHLLQMQSQS RSDLGKAAIR
NFQMERLAEA QLLCPTESTE RLRESQARSF KKFELLWHLG RDKQTARGFE KTLLKVLDTL
ALPHYMSERT FVTNWLQASL LRGDLARLTK PLYKILLSAS SKRVGIVHMQ QLYRESETEV
TPAFERDVYA ISSEQGNVKY HHMETSSGNK KKSPIRNFPK KIFGVTLSGG KANKVSNFVS
DKSTVATCSE ATQDMNTIGL IINPLENAND FDDETDLEEP RIEIPHKETP LEQKLASAMD
ESEQPSQEQP ANQPDNSLQY DQDITDHSDS SDFESDSELR ETSIEKEDSI TVSSSAGVSD
DVKRFVGDCE SVTDALTQHE KIKSRKTYRL TREKTPGENS LNSLEQKDHD SISELQADAL
PADEYFREDK KLGKRKKVLT SGEKKRLSCI SKTSTDSNIS GSHVEQPEQE EETEPGTEST
INVEDKRRNL SLETSKLQPD MQKTLEKGKQ NVEILRQNNA AAAAAAAAAA EEQISVRSSM
KSTVSLTDAA HLYHNHLLIY LAIYDTRQTL YALQTLRNII CCDKRTFLCL SITTSFSSAS
LKQLLVRHRK SISGKGFDGS VGNSEYAQGY RGCMQLELLV TLCLFYARGY FQKESLDAQR
QSPTLQDIVN NRRIQLESIE LLTLICSELI EIVKGMGRGL ANYIADLLAR AKLQKVMLHC
LNSSVCSYGL KGNTNSGSYA EQVLSFNDPQ DDQLHADCFQ LQLLRLLLAV IRLEHEVHQL
RQDTPPAAGE DSAGNASPTR LAEGATANVK YLPNCLISQQ PMFLAAVLGA LQQERLRHLH
RNWTDLVTSS LNCFSFGSLT NIVISVVHQL CSNLDRISKM GLPQQRHFPP DYVVSQLEAL
TILCHYCLLD NTQQTALSHL FNQAYPQTSS SAQSSSTGQL LNSIVHSFLS ASESSTSAPA
PRNPQLQAAR NAVLSHLPRI MSSVAAIWDS ELGQLRPVRQ QLLEFLSPVS LHHGSNFLAA
VAVTWQQRGI ATNMNGLSIA EQFQRNSVLQ ACPAQLSLVS LVSSIRVMPM DSFVMTLHHV
VRSPPPIHRP PAQLSIEVSA LELFYFYMKS APAPQLADAW SSLLALIRDG LNLTPPAQFA
LLMLLNEFVQ RCPQMPFQDK KEVRELHDVT SRLVDSLSSV AGSCLEQTTW LRRNLAVKED
TDGLAKDNSV GGGGLQQYSL QAQSVLAAIL SNLLDVAYGS QEKDKVVNIV TPLLYNITPY
LKNHTARNVP FFYACSALLA SLSGYQYTRK AWRKDMLDLL LDNAFFQMHL SCLPFWRMIM
DSLMTYDNTT FRELMTRVSL SQAGSLNIFT SRDQEYEQRA MLLKRLAFVI YCSEFDQHNK
YMPDIQEQLA NSLRLVPMGP SVQAAVFLCF RVLLLRMSPD HVTSLWPIII AEMVQVFLQM
EQELKSESEE RNQQLRLPSG IDVSWSAASG ASNGYNSQTP MQHWRSVQLE ACKLLELGCV
LPATKLPHFQ MYRWAFVGTE FDVHEEEVCL PNGSLENLAT LPSALYVPHV RRVARLMDMK
YTSQSPILQR PSNRHLMLHF QQLQSLQELY AFFTTLGISC PQPRNFADTE NDVANCLKEI
EEVLANDFLE KLPSITTPR
