DOP2_MOUSE
ID DOP2_MOUSE Reviewed; 2295 AA.
AC Q3UHQ6; E9QLV2; Q3UH78; Q80TN8;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein dopey-2;
GN Name=Dop1b; Synonyms=Dopey2 {ECO:0000312|MGI:MGI:1917278}, Kiaa0933;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 740-2295 (ISOFORMS 1/2).
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [4]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=12767918; DOI=10.1016/s0006-291x(03)00867-2;
RA Lopes C., Chettouh Z., Delabar J.-M., Rachidi M.;
RT "The differentially expressed C21orf5 gene in the medial temporal-lobe
RT system could play a role in mental retardation in Down syndrome and
RT transgenic mice.";
RL Biochem. Biophys. Res. Commun. 305:915-924(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1167, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-556; SER-597 AND SER-1167,
RP AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in regulating membrane trafficking of cargo
CC proteins. Together with ATP9A and MON2, regulates SNX3 retromer-
CC mediated endosomal sorting of WLS away from lysosomal degradation.
CC {ECO:0000250|UniProtKB:Q9Y3R5}.
CC -!- SUBUNIT: Homooligomer. Heterotrimer with ATP9A and MON2; this
CC interaction is retromer-independent. Interacts with SNX3.
CC {ECO:0000250|UniProtKB:Q9Y3R5}.
CC -!- SUBCELLULAR LOCATION: Early endosome membrane
CC {ECO:0000250|UniProtKB:Q9Y3R5}. Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q03921}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q03921}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3UHQ6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3UHQ6-2; Sequence=VSP_027430;
CC -!- TISSUE SPECIFICITY: Expressed in liver, heart and brain.
CC {ECO:0000269|PubMed:12767918}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in developing nervous system.
CC {ECO:0000269|PubMed:12767918}.
CC -!- SIMILARITY: Belongs to the dopey family. {ECO:0000305}.
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DR EMBL; AK147255; BAE27801.1; -; mRNA.
DR EMBL; AK147536; BAE27979.1; -; mRNA.
DR EMBL; AC154449; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC168220; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK122403; BAC65685.1; -; mRNA.
DR CCDS; CCDS28343.1; -. [Q3UHQ6-1]
DR RefSeq; NP_080976.1; NM_026700.2.
DR RefSeq; NP_081569.1; NM_027293.1. [Q3UHQ6-1]
DR RefSeq; XP_006523145.1; XM_006523082.3.
DR AlphaFoldDB; Q3UHQ6; -.
DR STRING; 10090.ENSMUSP00000044437; -.
DR iPTMnet; Q3UHQ6; -.
DR PhosphoSitePlus; Q3UHQ6; -.
DR EPD; Q3UHQ6; -.
DR jPOST; Q3UHQ6; -.
DR MaxQB; Q3UHQ6; -.
DR PaxDb; Q3UHQ6; -.
DR PeptideAtlas; Q3UHQ6; -.
DR PRIDE; Q3UHQ6; -.
DR ProteomicsDB; 279469; -. [Q3UHQ6-1]
DR ProteomicsDB; 279470; -. [Q3UHQ6-2]
DR Antibodypedia; 54117; 22 antibodies from 9 providers.
DR DNASU; 70028; -.
DR Ensembl; ENSMUST00000045004; ENSMUSP00000044437; ENSMUSG00000022946. [Q3UHQ6-1]
DR GeneID; 70028; -.
DR KEGG; mmu:70028; -.
DR UCSC; uc007zzv.1; mouse. [Q3UHQ6-1]
DR CTD; 9980; -.
DR MGI; MGI:1917278; Dop1b.
DR VEuPathDB; HostDB:ENSMUSG00000022946; -.
DR eggNOG; KOG3613; Eukaryota.
DR GeneTree; ENSGT00390000016421; -.
DR HOGENOM; CLU_001045_0_0_1; -.
DR InParanoid; Q3UHQ6; -.
DR OMA; NHSAYNT; -.
DR OrthoDB; 29961at2759; -.
DR PhylomeDB; Q3UHQ6; -.
DR TreeFam; TF316855; -.
DR BioGRID-ORCS; 70028; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Dop1b; mouse.
DR PRO; PR:Q3UHQ6; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q3UHQ6; protein.
DR Bgee; ENSMUSG00000022946; Expressed in granulocyte and 240 other tissues.
DR ExpressionAtlas; Q3UHQ6; baseline and differential.
DR Genevisible; Q3UHQ6; MM.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0031901; C:early endosome membrane; ISS:UniProtKB.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0050890; P:cognition; ISO:MGI.
DR GO; GO:0009880; P:embryonic pattern specification; ISO:MGI.
DR GO; GO:0006895; P:Golgi to endosome transport; IEA:InterPro.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR040314; DOP1.
DR InterPro; IPR007249; Dopey_N.
DR PANTHER; PTHR14042; PTHR14042; 1.
DR Pfam; PF04118; Dopey_N; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endosome; Golgi apparatus; Membrane; Phosphoprotein;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..2295
FT /note="Protein dopey-2"
FT /id="PRO_0000297949"
FT REGION 574..599
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 651..684
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1034..1059
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1092..1136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1034..1049
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1101..1132
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 556
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 597
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1167
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..118
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027430"
FT CONFLICT 721
FT /note="H -> Y (in Ref. 1; BAE27801)"
FT /evidence="ECO:0000305"
FT CONFLICT 791
FT /note="W -> G (in Ref. 1; BAE27801)"
FT /evidence="ECO:0000305"
FT CONFLICT 1251
FT /note="T -> S (in Ref. 1; BAE27801)"
FT /evidence="ECO:0000305"
FT CONFLICT 1876
FT /note="S -> TG (in Ref. 1; BAE27979)"
FT /evidence="ECO:0000305"
FT CONFLICT 1881
FT /note="S -> P (in Ref. 1; BAE27801)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2295 AA; 257483 MW; 631B31C304BCD58A CRC64;
MDPEEQELLN DYRYRSYSSV IEKALRNFES SSEWADLISS LGKLNKALQS NLKYSLLPRR
LIISKRLAQC LHPALPSGVH LKALETYEII FKIVGTKWLA KDLFLYSCGL FPLLAYAAMS
VRPVLLGLYE KYFLPLQKLL LPSLQAFLVG LLPGLEEGSE IYERTDALLL RLSVVVGREV
FYAALWGSVL TSPSIRLPAS LFVVNHISRD SPGKEQKCML GTDYQLTVRS LCASLLDANV
LVQRNNLEII LFFFPFYTCL DPEERAIPLL RRDVVHILSA ATQTLLRRDM SLNRRLYAWL
LGSDIKGNTI VPKSEISNSY EDQCSYFFDK YSKDLLVEAL AEILHQKFLD ADLEERHHAY
LKPFRILVSL LDKPEIGPQV VENLFLEVIR AFYSYCHDVL GSDLKLSYTQ SGNPLISTIK
ENRHASEIVK TVNLLVSSLS SDFLWDYMAR RFEACFRPVT QTTAIGEAIS PPPTVSELCT
LLVFLLDVIP LELYSEVQTQ YLPQVLGCLL QPLAEEVEAL SLPELTHALK TCFKVLSKVQ
MPPSYLDLEP SSGNSSPVKG RNSGIAMETE AVVAGDEEPS FPPLKSEDSG IGLSASSPEL
SEHLRVPRVS AERDDIWKKD GTMQATFLCI QELIANFASK NIFAASLTVS GEENKPEEPP
GKGNKGQTQS TEHPGRKSSW DPKPITVPQF KQMLSDLFTV RGSPFKTRSS ESLSSVPSSP
HRKAATEWGV DQVVVELVGS KEDCREALAA ACHLLLDCAT FPVYLSEEET EQLCETLFQT
PGASDCSFPP WLKSLMTICC CVSDCSLQNI AIATLLEVIN HSQSLALVIE DKMKRYKTSG
NNPFFGKLQM VTVPPIAPGI LKVIAEKTDF YQRVARVLWN QLNKETREHH ITCVELFYRL
HCLAPTANIC EDIICHALLD PDKGTRLEAL FRFSVIWHLT REIQGSRVTS HNRSFDRSLF
VVLDSLACTD GAISAAAQGW LVRALSLGDV ARILEPMLLL LLQPKTQRTS IQCLKQENSA
EDLHRWFNRK KPTCKEACGE SEPQEGAPEE RLPRGQFTTV DREAIWAEVE KEPEKCPPRS
DLSEEDLPYY VDLPDRMTSG GGDSSEHTES ADTSSGHTDS ENTSTFSSPS HDLQDLSHEE
NCCAPIPIGG RAYSKRAALL AAFQPESPRS NARLSLVRAD SDKTQASESF SSDEEADVEL
QAITTSRLLK QQREKQETVE ALFKHILLYL QPYDSQRVLY AFSVLEAVLK TNPKEFIEAV
SRTGIDTSST AHLNLISNLL ARHQEALIGQ SFYGKLQTQA PNVCPHSLLI ELLTYLCLSF
LRSYYPCYLK VCHRDILGNR DVQVKSVEVL IRITAQLVSM AKSAEGKNTE FIHSLLQRCK
VQEFVLLSLS ASMYTSQKRY GLATADRGGR LLAEDSLFEE SLINLGQDQI WSEHPLQIEL
LKLLQALIVL EHHLGQGQEE AETQPALSRE WQRALNFQQA IGAMQYVQPH PLTSQGLLVS
AVVRGLQPAY GYGMHPAWVS LVTHSLPYFG KSLGWTVTPF VIQICKNLDD LVKQYESESV
KFTISTTSKK ENISPDYPLT LLEGLTTISH FCLLEQPTQH KKTAAVSDPI NLRNAKNAIL
EELPRIVNTM ALIWNVLRKE ETQKRPVDLL GATKGSSSVY FKTTKTIRQK ILDLLNPLTG
HLGVQLIAAV ATVWNRKQAR RHSKTKMVPV ANTSQHTLVD LVCALSTLQT DSVLQLVKEV
VKRPAQIKGD EKSPLVDIPV LQFCYAFIQR LSIPDLQEAF PSLLGVLKEA AQLNLAPPGY
FLLLSMLNDF VTRTPNLESK KDQKDLQEIT QRILEAVGTI AGSSLEQTSW LSRNLEVKAQ
PQVSLEESDA EDDVHSAAEA STMVSASAPS VYSVQALSLL AEVLASLLDM VYRSDEKEKA
VPLISRLLYY VFPYLRNHSA YNAPSFRAGA QLLSSLSGYA YTKRAWKKEV LELFLDPAFF
QMDTSCVHWK SVIDHLLTHE KTMFKDLMNM QSSSLKLFSS FEQKAMLLKR QAFAVFSGEL
DQYHLYLPLI QERLTDNLRV GQTSIVAGQM FLFFRVLLLR ISPQHLTSLW PIMVSELIQT
FIQLEEDLKE EDESRNSHKS NRIKAPVADG NGSAGRVLSP SDLTMYLSAC KFLDTALAFP
PDKMPLFQIY RWAFVPEVDT EHPAFLSELE ENHQECRPHT VRILELLRSR YGEIGSSDEI
TRKKEFPLLR QHSVSCIRQL IPFFTTLNCA FKTQSQLPAD VPGTAAPECP VTENPRVLRQ
LEECVEQDFL EHPEC
