DOPR2_DROME
ID DOPR2_DROME Reviewed; 539 AA.
AC Q24563; Q24569; Q9VAJ8;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=Dopamine receptor 2;
DE AltName: Full=Dopamine 1-like receptor 2;
GN Name=Dop1R2; Synonyms=DAMB, DopR2, DopR99B; ORFNames=CG18741;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), AND FUNCTION.
RC STRAIN=Canton-S; TISSUE=Head;
RX PubMed=8656286; DOI=10.1523/jneurosci.16-12-03925.1996;
RA Feng G., Hannan F., Reale V., Hon Y.Y., Kousky C.T., Evans P.D., Hall L.M.;
RT "Cloning and functional characterization of a novel dopamine receptor from
RT Drosophila melanogaster.";
RL J. Neurosci. 16:3925-3933(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S;
RX PubMed=8663989; DOI=10.1016/s0896-6273(00)80139-7;
RA Han K.-A., Millar N.S., Grotewiel M.S., Davis R.L.;
RT "DAMB, a novel dopamine receptor expressed specifically in Drosophila
RT mushroom bodies.";
RL Neuron 16:1127-1135(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Receptor for dopamine. The activity of this receptor is
CC mediated by G proteins which activate adenylyl cyclase. Also capable of
CC generating a calcium signal. In terms of antagonist responses, would be
CC classed with the D1-like dopamine receptor group. This receptor is an
CC attractive candidate for initiating biochemical cascades underlying
CC olfactory learning. {ECO:0000269|PubMed:8656286,
CC ECO:0000269|PubMed:8663989}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=Q24563-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q24563-2; Sequence=VSP_001877;
CC -!- TISSUE SPECIFICITY: Expressed in both central and peripheral nervous
CC systems. {ECO:0000269|PubMed:8663989}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; U34383; AAC47161.1; -; mRNA.
DR EMBL; U61264; AAB08000.1; -; mRNA.
DR EMBL; AE014297; AAF56908.2; -; Genomic_DNA.
DR EMBL; AE014297; AAN14180.1; -; Genomic_DNA.
DR EMBL; BT010212; AAQ23530.1; -; mRNA.
DR RefSeq; NP_524548.1; NM_079824.4. [Q24563-1]
DR RefSeq; NP_733299.1; NM_170420.2. [Q24563-2]
DR AlphaFoldDB; Q24563; -.
DR SMR; Q24563; -.
DR BioGRID; 68348; 1.
DR DIP; DIP-22945N; -.
DR STRING; 7227.FBpp0084834; -.
DR GlyGen; Q24563; 4 sites.
DR PaxDb; Q24563; -.
DR EnsemblMetazoa; FBtr0085467; FBpp0084833; FBgn0266137. [Q24563-2]
DR EnsemblMetazoa; FBtr0085468; FBpp0084834; FBgn0266137. [Q24563-1]
DR GeneID; 43484; -.
DR KEGG; dme:Dmel_CG18741; -.
DR CTD; 43484; -.
DR FlyBase; FBgn0266137; Dop1R2.
DR VEuPathDB; VectorBase:FBgn0266137; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000154484; -.
DR InParanoid; Q24563; -.
DR OMA; YVHEQRA; -.
DR PhylomeDB; Q24563; -.
DR Reactome; R-DME-381753; Olfactory Signaling Pathway.
DR Reactome; R-DME-390696; Adrenoceptors.
DR Reactome; R-DME-416476; G alpha (q) signalling events.
DR Reactome; R-DME-416482; G alpha (12/13) signalling events.
DR BioGRID-ORCS; 43484; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 43484; -.
DR PRO; PR:Q24563; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0266137; Expressed in brain and 6 other tissues.
DR ExpressionAtlas; Q24563; baseline and differential.
DR Genevisible; Q24563; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:FlyBase.
DR GO; GO:0030285; C:integral component of synaptic vesicle membrane; IC:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:FlyBase.
DR GO; GO:0004935; F:adrenergic receptor activity; IEA:InterPro.
DR GO; GO:0004952; F:dopamine neurotransmitter receptor activity; ISS:FlyBase.
DR GO; GO:0001588; F:dopamine neurotransmitter receptor activity, coupled via Gs; IDA:UniProtKB.
DR GO; GO:0008227; F:G protein-coupled amine receptor activity; ISS:FlyBase.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0008226; F:tyramine receptor activity; IDA:CACAO.
DR GO; GO:0071880; P:adenylate cyclase-activating adrenergic receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007191; P:adenylate cyclase-activating dopamine receptor signaling pathway; IDA:FlyBase.
DR GO; GO:0001306; P:age-dependent response to oxidative stress; IMP:FlyBase.
DR GO; GO:1903351; P:cellular response to dopamine; IMP:FlyBase.
DR GO; GO:0007212; P:dopamine receptor signaling pathway; ISS:FlyBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:UniProtKB.
DR GO; GO:0042321; P:negative regulation of circadian sleep/wake cycle, sleep; IMP:FlyBase.
DR GO; GO:1903223; P:positive regulation of oxidative stress-induced neuron death; IMP:FlyBase.
DR GO; GO:0099509; P:regulation of presynaptic cytosolic calcium ion concentration; IMP:FlyBase.
DR GO; GO:1990834; P:response to odorant; IMP:FlyBase.
DR InterPro; IPR002233; ADR_fam.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR01103; ADRENERGICR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Lipoprotein; Membrane; Palmitate;
KW Phosphoprotein; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..539
FT /note="Dopamine receptor 2"
FT /id="PRO_0000069370"
FT TOPO_DOM 1..113
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..145
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..189
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..206
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..227
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..266
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..420
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 442..453
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 454..474
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 475..539
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 326..387
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 492
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 493
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT CARBOHYD 5
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 31
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 68
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 182..261
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VAR_SEQ 511..539
FT /note="RFATRRCYSTCSLHGIQHVRHNSCEQTYI -> CHVAAAMVAASTSFGYHSV
FT NQIDRTLM (in isoform B)"
FT /evidence="ECO:0000303|PubMed:8663989"
FT /id="VSP_001877"
FT CONFLICT 496
FT /note="K -> E (in Ref. 5; AAQ23530)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 539 AA; 59505 MW; 32FDDC0E935AF4B3 CRC64;
MVDDNGSSPE VEGAEGAGAP LLALLRVDGL NQTQTRSPSP SFFGSYNISE DVYFYFNGLP
TSTELVLNAT TSATSATLSP AMVATGGGGT TTPEPDLSEF LEALPNDRVG LLAFLFLFSF
ATVFGNSLVI LAVIRERYLH TATNYFITSL AVADCLVGLV VMPFSALYEV LENTWFFGTD
WCDIWRSLDV LFSTASILNL CVISLDRYWA ITDPFSYPMR MTVKRAAGLI AAVWICSSAI
SFPAIVWWRA ARDGEMPAYK CTFTEHLGYL VFSSTISFYL PLLVMVFTYC RIYRAAVIQT
RSLKIGTKQV LMASGELQLT LRIHRGGTTR DQQNQVSGGG GGGGGGGGGG GSLSHSHSHS
HHHHHNHGGG TTTSTPEEPD DEPLSALHNN GLARHRHMGK NFSLSRKLAK FAKEKKAAKT
LGIVMGVFII CWLPFFVVNL LSGFCIECIE HEEIVSAIVT WLGWINSCMN PVIYACWSRD
FRRAFVRLLC MCCPRKIRRK YQPTMRSKSQ RFATRRCYST CSLHGIQHVR HNSCEQTYI
