DOS2_YEAST
ID DOS2_YEAST Reviewed; 310 AA.
AC P54858; D6VS54;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Protein DOS2;
GN Name=DOS2; OrderedLocusNames=YDR068W; ORFNames=D4267, YD8554.01, YD9609.22;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8657109; DOI=10.1128/mcb.16.4.1356;
RA Singer J.D., Manning B.M., Formosa T.;
RT "Coordinating DNA replication to produce one copy of the genome requires
RT genes that act in ubiquitin metabolism.";
RL Mol. Cell. Biol. 16:1356-1366(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8789263;
RX DOI=10.1002/(sici)1097-0061(199601)12:1<85::aid-yea890>3.0.co;2-u;
RA Brandt P., Ramlow S., Otto B., Bloecker H.;
RT "Nucleotide sequence analysis of a 32,500 bp region of the right arm of
RT Saccharomyces cerevisiae chromosome IV.";
RL Yeast 12:85-90(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Acts in ubiquitin metabolism and is necessary for the control
CC of single-copy DNA replication.
CC -!- MISCELLANEOUS: Present with 3270 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U19857; AAA66522.1; -; Genomic_DNA.
DR EMBL; X84162; CAA58984.1; -; Genomic_DNA.
DR EMBL; Z74364; CAA98886.1; -; Genomic_DNA.
DR EMBL; Z49209; CAA89097.1; -; Genomic_DNA.
DR EMBL; Z46796; CAA86790.1; -; Genomic_DNA.
DR EMBL; AY557687; AAS56013.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11914.1; -; Genomic_DNA.
DR PIR; S54052; S54052.
DR RefSeq; NP_010353.3; NM_001180376.3.
DR AlphaFoldDB; P54858; -.
DR SMR; P54858; -.
DR BioGRID; 32123; 73.
DR DIP; DIP-4785N; -.
DR IntAct; P54858; 23.
DR MINT; P54858; -.
DR STRING; 4932.YDR068W; -.
DR iPTMnet; P54858; -.
DR MaxQB; P54858; -.
DR PaxDb; P54858; -.
DR PRIDE; P54858; -.
DR EnsemblFungi; YDR068W_mRNA; YDR068W; YDR068W.
DR GeneID; 851640; -.
DR KEGG; sce:YDR068W; -.
DR SGD; S000002475; DOS2.
DR VEuPathDB; FungiDB:YDR068W; -.
DR eggNOG; KOG2690; Eukaryota.
DR HOGENOM; CLU_064795_0_0_1; -.
DR InParanoid; P54858; -.
DR OMA; LFWYRYF; -.
DR BioCyc; YEAST:G3O-29675-MON; -.
DR PRO; PR:P54858; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P54858; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR Gene3D; 1.10.3970.10; -; 1.
DR InterPro; IPR005607; BSD_dom.
DR InterPro; IPR035925; BSD_dom_sf.
DR Pfam; PF03909; BSD; 1.
DR SMART; SM00751; BSD; 1.
DR SUPFAM; SSF140383; SSF140383; 1.
DR PROSITE; PS50858; BSD; 1.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..310
FT /note="Protein DOS2"
FT /id="PRO_0000079981"
FT DOMAIN 176..228
FT /note="BSD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00036"
FT REGION 15..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 131..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 240..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..146
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 248..264
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 310 AA; 35964 MW; B9B318093E984713 CRC64;
MEFFYEEQVA CIEDDKISNS HTKETGSTEN TENNELQSRD DKTNEAFQKL EEEVNKRYEK
TTSAFKKLVI EKDDGIEINL PISNETTETA QKYLKKLDEN IHSVESLAQS YWSKMKTKNF
WSGFSSFDNA AENDSNDKDE NSKENEIAVG GNRTEAELRT LSKDKSVYLD NKMDLQLDPF
DVDEKTEEIC SILQGDKDIS KLMNDIVPHK ISYKDFWHIY FLQRNKILDK ESKRKEILSK
KEKETEEKEV EWDDEEEEED DDKVEAVADN KSKGETKVAV SQEGLKDVSD HVGLANKDES
KDDDDDDDWE
