DOT2_ARATH
ID DOT2_ARATH Reviewed; 820 AA.
AC Q9LFE0; B9DHM8; Q9ASS0;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=SART-1 family protein DOT2 {ECO:0000305};
DE AltName: Full=Protein DEFECTIVELY ORGANIZED TRIBUTARIES 2 {ECO:0000303|PubMed:18643975};
DE AltName: Full=Protein MERISTEM-DEFECTIVE {ECO:0000303|PubMed:19000164};
GN Name=DOT2 {ECO:0000303|PubMed:18643975};
GN Synonyms=MDF {ECO:0000303|PubMed:19000164};
GN OrderedLocusNames=At5g16780 {ECO:0000312|Araport:AT5G16780};
GN ORFNames=F5E19.120 {ECO:0000312|EMBL:CAC01842.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-652.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 22-820.
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=18643975; DOI=10.1111/j.1365-313x.2008.03595.x;
RA Petricka J.J., Clay N.K., Nelson T.M.;
RT "Vein patterning screens and the defectively organized tributaries mutants
RT in Arabidopsis thaliana.";
RL Plant J. 56:251-263(2008).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19000164; DOI=10.1111/j.1365-313x.2008.03738.x;
RA Casson S.A., Topping J.F., Lindsey K.;
RT "MERISTEM-DEFECTIVE, an RS domain protein, is required for the correct
RT meristem patterning and function in Arabidopsis.";
RL Plant J. 57:857-869(2009).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Plays a role in root, shoot and flower development. Probably
CC required for normal root and shoot meristem organization and
CC maintenance and the proper expression of PIN and PLT genes
CC (PubMed:19000164). Involved in leaf vasculature patterning
CC (PubMed:18643975). {ECO:0000269|PubMed:18643975,
CC ECO:0000269|PubMed:19000164}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19000164}.
CC -!- TISSUE SPECIFICITY: Expressed in lateral root cap, columella, meristem
CC and quiescent center (QC) (PubMed:19000164). Expressed in young leaves
CC (PubMed:18643975). {ECO:0000269|PubMed:18643975,
CC ECO:0000269|PubMed:19000164}.
CC -!- DISRUPTION PHENOTYPE: Dwarf plants with variable number of cotyledons,
CC short petioles in leaves and impaired flowering (PubMed:18643975,
CC PubMed:19000164). Defects in venation pattern in leaves
CC (PubMed:18643975). {ECO:0000269|PubMed:18643975,
CC ECO:0000269|PubMed:19000164}.
CC -!- SIMILARITY: Belongs to the SNU66/SART1 family. {ECO:0000305}.
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DR EMBL; AL391147; CAC01842.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92337.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68274.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68275.1; -; Genomic_DNA.
DR EMBL; AF367317; AAK32904.1; -; mRNA.
DR EMBL; AK317581; BAH20245.1; -; mRNA.
DR PIR; T51510; T51510.
DR RefSeq; NP_001318573.1; NM_001343464.1.
DR RefSeq; NP_001330040.1; NM_001343465.1.
DR RefSeq; NP_197180.1; NM_121684.4.
DR AlphaFoldDB; Q9LFE0; -.
DR BioGRID; 16817; 1.
DR IntAct; Q9LFE0; 1.
DR STRING; 3702.AT5G16780.1; -.
DR iPTMnet; Q9LFE0; -.
DR PaxDb; Q9LFE0; -.
DR PRIDE; Q9LFE0; -.
DR ProteomicsDB; 222138; -.
DR EnsemblPlants; AT5G16780.1; AT5G16780.1; AT5G16780.
DR EnsemblPlants; AT5G16780.2; AT5G16780.2; AT5G16780.
DR EnsemblPlants; AT5G16780.3; AT5G16780.3; AT5G16780.
DR GeneID; 831541; -.
DR Gramene; AT5G16780.1; AT5G16780.1; AT5G16780.
DR Gramene; AT5G16780.2; AT5G16780.2; AT5G16780.
DR Gramene; AT5G16780.3; AT5G16780.3; AT5G16780.
DR KEGG; ath:AT5G16780; -.
DR Araport; AT5G16780; -.
DR TAIR; locus:2149010; AT5G16780.
DR eggNOG; KOG2217; Eukaryota.
DR HOGENOM; CLU_009379_0_0_1; -.
DR InParanoid; Q9LFE0; -.
DR OMA; EYARWEN; -.
DR OrthoDB; 1068640at2759; -.
DR PhylomeDB; Q9LFE0; -.
DR PRO; PR:Q9LFE0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LFE0; baseline and differential.
DR Genevisible; Q9LFE0; AT.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IBA:GO_Central.
DR GO; GO:0010588; P:cotyledon vascular tissue pattern formation; IMP:TAIR.
DR GO; GO:0009908; P:flower development; IMP:TAIR.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0010305; P:leaf vascular tissue pattern formation; IMP:TAIR.
DR GO; GO:0000481; P:maturation of 5S rRNA; IBA:GO_Central.
DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0010087; P:phloem or xylem histogenesis; IMP:TAIR.
DR GO; GO:0048528; P:post-embryonic root development; IMP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0048367; P:shoot system development; IMP:TAIR.
DR InterPro; IPR005011; SNU66/SART1.
DR PANTHER; PTHR14152; PTHR14152; 1.
DR Pfam; PF03343; SART-1; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Developmental protein; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..820
FT /note="SART-1 family protein DOT2"
FT /id="PRO_0000431315"
FT REGION 1..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 420..445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 523..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 564..617
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 657..678
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 729..748
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 762..820
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 58..120
FT /evidence="ECO:0000255"
FT COILED 171..235
FT /evidence="ECO:0000255"
FT COILED 433..510
FT /evidence="ECO:0000255"
FT COMPBIAS 1..158
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..224
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 426..445
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 564..595
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 603..617
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 734..748
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 796..820
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 22
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 820 AA; 94142 MW; 8639B9D89B460F3E CRC64;
MEVEKSKSRH EIREERADYE GSPVREHRDG RRKEKDHRSK DKEKDYDREK IRDKDHRRDK
EKERDRKRSR DEDTEKEISR GRDKEREKDK SRDRVKEKDK EKERNRHKDR ENERDNEKEK
DKDRARVKER ASKKSHEDDD ETHKAAERYE HSDNRGLNEG GDNVDAASSG KEASALDLQN
RILKMREERK KKAEDASDAL SWVARSRKIE EKRNAEKQRA QQLSRIFEEQ DNLNQGENED
GEDGEHLSGV KVLHGLEKVV EGGAVILTLK DQSVLTDGDV NNEIDMLENV EIGEQKRRNE
AYEAAKKKKG IYDDKFNDDP GAEKKMLPQY DEAATDEGIF LDAKGRFTGE AEKKLEELRK
RIQGQTTHTF EDLNSSAKVS SDYFSQEEML KFKKPKKKKQ LRKKDKLDLS MLEAEAVASG
LGAEDLGSRK DGRRQAMKEE KERIEYEKRS NAYQEAIAKA DEASRLLRRE QVQPFKRDED
ESMVLADDAE DLYKSLEKAR RLALIKKEEA GSGPQAVAHL VASSTNQTTD DNTTTGDETQ
ENTVVFTEMG DFVWGLQREN DVRKPESEDV FMEEDVAPKA PVEVKEEHPD GLTEVNDTDM
DAAEDSSDTK EITPDENIHE VAVGKGLSGA LKLLKDRGTL KEKVEWGGRN MDKKKSKLVG
IVDDDGGKES KDKESKDRFK DIRIERTDEF GRTLTPKEAF RLLSHKFHGK GPGKMKEEKR
MKQYQEELKL KQMKNSDTPS QSVQRMREAQ AQLKTPYLVL SGHVKPGQTS DPQSGFATVE
KDVPGSLTPM LGDRKVEHFL GIKRKSEPGN SDTPPKRPKP
