DOTM_LEGPH
ID DOTM_LEGPH Reviewed; 380 AA.
AC Q5ZYC7;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Type 4 apparatus protein DotM {ECO:0000303|PubMed:22694730};
GN Name=dotM {ECO:0000303|PubMed:17040490};
GN Synonyms=icmP {ECO:0000312|EMBL:AAU26542.1};
GN OrderedLocusNames=lpg0445 {ECO:0000312|EMBL:AAU26542.1};
OS Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC
OS 33152 / DSM 7513).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
OC Legionellaceae; Legionella.
OX NCBI_TaxID=272624;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Philadelphia 1 / ATCC 33152 / DSM 7513;
RX PubMed=15448271; DOI=10.1126/science.1099776;
RA Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G.,
RA Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V.,
RA Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S., Georghiou A.,
RA Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A.,
RA Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A.,
RA Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A.,
RA Russo J.J.;
RT "The genomic sequence of the accidental pathogen Legionella pneumophila.";
RL Science 305:1966-1968(2004).
RN [2]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=Philadelphia 1 / Lp02;
RX PubMed=17040490; DOI=10.1111/j.1365-2958.2006.05446.x;
RA Vincent C.D., Friedman J.R., Jeong K.C., Buford E.C., Miller J.L.,
RA Vogel J.P.;
RT "Identification of the core transmembrane complex of the Legionella Dot/Icm
RT type IV secretion system.";
RL Mol. Microbiol. 62:1278-1291(2006).
RN [3]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=Philadelphia 1 / Lp02;
RX PubMed=22694730; DOI=10.1111/j.1365-2958.2012.08118.x;
RA Vincent C.D., Friedman J.R., Jeong K.C., Sutherland M.C., Vogel J.P.;
RT "Identification of the DotL coupling protein subcomplex of the Legionella
RT Dot/Icm type IV secretion system.";
RL Mol. Microbiol. 85:378-391(2012).
RN [4] {ECO:0007744|PDB:5X1U}
RP X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 158-365, AND SUBUNIT.
RC STRAIN=Philadelphia 1 / ATCC 33152 / DSM 7513;
RX PubMed=28714967; DOI=10.1038/nmicrobiol.2017.114;
RA Kwak M.J., Kim J.D., Kim H., Kim C., Bowman J.W., Kim S., Joo K., Lee J.,
RA Jin K.S., Kim Y.G., Lee N.K., Jung J.U., Oh B.H.;
RT "Architecture of the type IV coupling protein complex of Legionella
RT pneumophila.";
RL Nat. Microbiol. 2:17114-17114(2017).
RN [5] {ECO:0007744|PDB:6EXA, ECO:0007744|PDB:6EXB, ECO:0007744|PDB:6EXC, ECO:0007744|PDB:6EXD, ECO:0007744|PDB:6EXE}
RP X-RAY CRYSTALLOGRAPHY (1.79 ANGSTROMS) OF 153-380 OF WILD-TYPE AND MUTANTS,
RP FUNCTION, DOMAIN, AND MUTAGENESIS OF 196-ARG-ARG-197 AND ARG-217.
RX PubMed=29410427; DOI=10.1038/s41467-017-02578-x;
RA Meir A., Chetrit D., Liu L., Roy C.R., Waksman G.;
RT "Legionella DotM structure reveals a role in effector recruiting to the
RT Type 4B secretion system.";
RL Nat. Commun. 9:507-507(2018).
RN [6] {ECO:0007744|PDB:6SZ9}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.70 ANGSTROMS), FUNCTION, SUBUNIT, AND
RP MUTAGENESIS OF THR-205; LEU-208; TYR-211; 300-VAL--SER-303 AND
RP 326-GLN-THR-327.
RC STRAIN=Philadelphia 1 / Lp01;
RX PubMed=32513920; DOI=10.1038/s41467-020-16681-z;
RA Meir A., Mace K., Lukoyanova N., Chetrit D., Hospenthal M.K., Redzej A.,
RA Roy C., Waksman G.;
RT "Mechanism of effector capture and delivery by the type IV secretion system
RT from Legionella pneumophila.";
RL Nat. Commun. 11:2864-2864(2020).
CC -!- FUNCTION: Component of the Dot/Icm type IVB secretion system (T4BSS),
CC which is used to inject bacterial effector proteins into eukaryotic
CC host cells (PubMed:17040490, PubMed:22694730, PubMed:32513920). Part of
CC a subcomplex which recruits effector proteins and delivers them to the
CC core transmembrane subcomplex (PubMed:32513920). Forms the interacting
CC surface for recruitment of acidic Glu-rich motif-containing effectors
CC (PubMed:29410427). {ECO:0000269|PubMed:17040490,
CC ECO:0000269|PubMed:22694730, ECO:0000269|PubMed:29410427,
CC ECO:0000269|PubMed:32513920}.
CC -!- SUBUNIT: The T4BSS is a complex nanomachine composed of several
CC subcomplexes. This subunit is part of the Type IV Coupling Complex
CC (T4CC), a subcomplex composed of the DotLMNYZ core and the IcmSW-LvgA
CC adapter subunits, linked by the C-terminal tail of DotL
CC (PubMed:17040490, PubMed:22694730, PubMed:28714967, PubMed:32513920).
CC Six DotLMNYZ hetero-pentameric units may assemble into an hexameric
CC nanomachine, forming an inner membrane channel for effectors to pass
CC through (PubMed:32513920). Interacts directly with DotL
CC (PubMed:22694730, PubMed:32513920). {ECO:0000269|PubMed:17040490,
CC ECO:0000269|PubMed:22694730, ECO:0000269|PubMed:28714967,
CC ECO:0000269|PubMed:32513920}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:17040490,
CC ECO:0000269|PubMed:22694730}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Localizes to the poles of the cell.
CC {ECO:0000269|PubMed:22694730}.
CC -!- DOMAIN: Exhibits patches of positively charged residues, which form
CC binding sites for acidic Glu-rich peptides.
CC {ECO:0000269|PubMed:29410427}.
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DR EMBL; AE017354; AAU26542.1; -; Genomic_DNA.
DR RefSeq; YP_094489.1; NC_002942.5.
DR PDB; 5X1U; X-ray; 1.80 A; A/B=158-365.
DR PDB; 6EXA; X-ray; 1.79 A; A/B=153-380.
DR PDB; 6EXB; X-ray; 1.84 A; A/B=153-380.
DR PDB; 6EXC; X-ray; 2.16 A; A/B=153-380.
DR PDB; 6EXD; X-ray; 2.14 A; A/B=153-380.
DR PDB; 6EXE; X-ray; 2.00 A; A/B=153-380.
DR PDB; 6SZ9; EM; 3.70 A; B=1-380.
DR PDB; 7OVB; EM; 3.61 A; B=1-380.
DR PDBsum; 5X1U; -.
DR PDBsum; 6EXA; -.
DR PDBsum; 6EXB; -.
DR PDBsum; 6EXC; -.
DR PDBsum; 6EXD; -.
DR PDBsum; 6EXE; -.
DR PDBsum; 6SZ9; -.
DR PDBsum; 7OVB; -.
DR SMR; Q5ZYC7; -.
DR STRING; 272624.lpg0445; -.
DR TCDB; 3.A.7.9.1; the type iv (conjugal dna-protein transfer or virb) secretory pathway (ivsp) family.
DR PaxDb; Q5ZYC7; -.
DR DNASU; 3077691; -.
DR EnsemblBacteria; AAU26542; AAU26542; lpg0445.
DR KEGG; lpn:lpg0445; -.
DR PATRIC; fig|272624.6.peg.461; -.
DR eggNOG; ENOG5031YVT; Bacteria.
DR HOGENOM; CLU_733164_0_0_6; -.
DR OMA; EEMTAGI; -.
DR Proteomes; UP000000609; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Virulence.
FT CHAIN 1..380
FT /note="Type 4 apparatus protein DotM"
FT /id="PRO_0000455591"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 196..197
FT /note="RR->EE: Results in a significant decrease in
FT replication in macrophages. Displays lower levels of
FT effector translocation."
FT /evidence="ECO:0000269|PubMed:29410427"
FT MUTAGEN 205
FT /note="T->R: Abolishes intracellular growth in
FT A.castellanii; when associated with R-208 and R-211."
FT /evidence="ECO:0000269|PubMed:32513920"
FT MUTAGEN 208
FT /note="L->R: Abolishes intracellular growth in
FT A.castellanii; when associated with R-205 and R-211."
FT /evidence="ECO:0000269|PubMed:32513920"
FT MUTAGEN 211
FT /note="Y->R: Abolishes intracellular growth in
FT A.castellanii; when associated with R-205 and R-208."
FT /evidence="ECO:0000269|PubMed:32513920"
FT MUTAGEN 217
FT /note="R->E: Results in a significant decrease in
FT replication in macrophages. Displays lower levels of
FT effector translocation."
FT /evidence="ECO:0000269|PubMed:29410427"
FT MUTAGEN 300..303
FT /note="VVPS->RRPR: Abolishes intracellular growth in
FT A.castellanii."
FT /evidence="ECO:0000269|PubMed:32513920"
FT MUTAGEN 326..327
FT /note="QT->RR: Abolishes intracellular growth in
FT A.castellanii."
FT /evidence="ECO:0000269|PubMed:32513920"
SQ SEQUENCE 380 AA; 43814 MW; B8E1FDC5D577ED16 CRC64;
MYIEMAQQQQ QSGSDNSMAP VWIVILLFIT AYFVWALAHQ YIVSFVFTIN IWQARLVNLF
LNNQLLANQI YLMQTLDPNT VNWDQMVTVM RAVGDYMRYP VICILVVLAF VLYNSNVTLK
YRKTYDMKSL RAQEQFNWPA IMPIVKEDLV SQDVNKGPWA MALTPMEFAR KYNLLRKDDA
LLDNPVPGEE MTAGIRRGDA KRVFTMQLGP YWDGFERCSP QAYALSAVFM ARMNRDRDAA
NNILKVLDKT FVDGKPDFSV ARPVMKKYQN SELVQEVVAK HAYVLTVIAS LLEAAREDGV
VPSSEFLWLK PVDRRLWYML NCVGRQTPYS EVAGPFAHWK AEKEMGRRSL VPMIDEAIRA
LEIAVKEVRL TPRQMEELEP
