DOTN_LEGPH
ID DOTN_LEGPH Reviewed; 214 AA.
AC Q5ZYB7;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Type 4 apparatus protein DotN {ECO:0000303|PubMed:22694730};
GN Name=dotN {ECO:0000303|PubMed:17040490};
GN Synonyms=icmJ {ECO:0000312|EMBL:AAU26552.1};
GN OrderedLocusNames=lpg0455 {ECO:0000312|EMBL:AAU26552.1};
OS Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC
OS 33152 / DSM 7513).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
OC Legionellaceae; Legionella.
OX NCBI_TaxID=272624;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Philadelphia 1 / ATCC 33152 / DSM 7513;
RX PubMed=15448271; DOI=10.1126/science.1099776;
RA Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G.,
RA Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V.,
RA Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S., Georghiou A.,
RA Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A.,
RA Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A.,
RA Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A.,
RA Russo J.J.;
RT "The genomic sequence of the accidental pathogen Legionella pneumophila.";
RL Science 305:1966-1968(2004).
RN [2]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=Philadelphia 1 / Lp02;
RX PubMed=17040490; DOI=10.1111/j.1365-2958.2006.05446.x;
RA Vincent C.D., Friedman J.R., Jeong K.C., Buford E.C., Miller J.L.,
RA Vogel J.P.;
RT "Identification of the core transmembrane complex of the Legionella Dot/Icm
RT type IV secretion system.";
RL Mol. Microbiol. 62:1278-1291(2006).
RN [3]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=Philadelphia 1 / Lp02;
RX PubMed=22694730; DOI=10.1111/j.1365-2958.2012.08118.x;
RA Vincent C.D., Friedman J.R., Jeong K.C., Sutherland M.C., Vogel J.P.;
RT "Identification of the DotL coupling protein subcomplex of the Legionella
RT Dot/Icm type IV secretion system.";
RL Mol. Microbiol. 85:378-391(2012).
RN [4] {ECO:0007744|PDB:5X1H, ECO:0007744|PDB:5X42}
RP X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 13-207 AND 2-214 IN COMPLEXES
RP WITH ZINC AND DOTL, SUBUNIT, AND INTERACTION WITH DOTL.
RC STRAIN=Philadelphia 1 / ATCC 33152 / DSM 7513;
RX PubMed=28714967; DOI=10.1038/nmicrobiol.2017.114;
RA Kwak M.J., Kim J.D., Kim H., Kim C., Bowman J.W., Kim S., Joo K., Lee J.,
RA Jin K.S., Kim Y.G., Lee N.K., Jung J.U., Oh B.H.;
RT "Architecture of the type IV coupling protein complex of Legionella
RT pneumophila.";
RL Nat. Microbiol. 2:17114-17114(2017).
RN [5] {ECO:0007744|PDB:6SZ9}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.70 ANGSTROMS) OF 7-214, FUNCTION,
RP SUBUNIT, AND MUTAGENESIS OF 200-GLN--ASP-214.
RC STRAIN=Philadelphia 1 / Lp01;
RX PubMed=32513920; DOI=10.1038/s41467-020-16681-z;
RA Meir A., Mace K., Lukoyanova N., Chetrit D., Hospenthal M.K., Redzej A.,
RA Roy C., Waksman G.;
RT "Mechanism of effector capture and delivery by the type IV secretion system
RT from Legionella pneumophila.";
RL Nat. Commun. 11:2864-2864(2020).
CC -!- FUNCTION: Component of the Dot/Icm type IVB secretion system (T4BSS),
CC which is used to inject bacterial effector proteins into eukaryotic
CC host cells (PubMed:17040490, PubMed:22694730, PubMed:32513920). Part of
CC a subcomplex which recruits effector proteins and delivers them to the
CC core transmembrane subcomplex (PubMed:32513920).
CC {ECO:0000269|PubMed:17040490, ECO:0000269|PubMed:22694730,
CC ECO:0000269|PubMed:32513920}.
CC -!- SUBUNIT: The T4BSS is a complex nanomachine composed of several
CC subcomplexes. This subunit is part of the Type IV Coupling Complex
CC (T4CC), a subcomplex composed of the DotLMNYZ core and the IcmSW-LvgA
CC adapter subunits, linked by the C-terminal tail of DotL
CC (PubMed:17040490, PubMed:22694730, PubMed:28714967, PubMed:32513920).
CC Six DotLMNYZ hetero-pentameric units may assemble into an hexameric
CC nanomachine, forming an inner membrane channel for effectors to pass
CC through (PubMed:32513920). Interacts directly with DotL
CC (PubMed:28714967, PubMed:32513920). Interacts with DotZ
CC (PubMed:32513920). {ECO:0000269|PubMed:17040490,
CC ECO:0000269|PubMed:22694730, ECO:0000269|PubMed:28714967,
CC ECO:0000269|PubMed:32513920}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17040490}.
CC Note=Localizes to the poles of the cell. {ECO:0000305|PubMed:22694730}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE017354; AAU26552.1; -; Genomic_DNA.
DR RefSeq; YP_094499.1; NC_002942.5.
DR PDB; 5X1H; X-ray; 3.00 A; A/C/E/G/I/K/M/O/S/U/W=7-214, Q=13-207.
DR PDB; 5X42; X-ray; 1.80 A; A=13-207, C=2-214.
DR PDB; 6SZ9; EM; 3.70 A; C=7-214.
DR PDB; 7OVB; EM; 3.61 A; C=7-214.
DR PDBsum; 5X1H; -.
DR PDBsum; 5X42; -.
DR PDBsum; 6SZ9; -.
DR PDBsum; 7OVB; -.
DR SMR; Q5ZYB7; -.
DR STRING; 272624.lpg0455; -.
DR TCDB; 3.A.7.9.1; the type iv (conjugal dna-protein transfer or virb) secretory pathway (ivsp) family.
DR PaxDb; Q5ZYB7; -.
DR EnsemblBacteria; AAU26552; AAU26552; lpg0455.
DR KEGG; lpn:lpg0455; -.
DR PATRIC; fig|272624.6.peg.471; -.
DR eggNOG; COG1403; Bacteria.
DR HOGENOM; CLU_096064_0_0_6; -.
DR OMA; QCFFIES; -.
DR Proteomes; UP000000609; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR003615; HNH_nuc.
DR SMART; SM00507; HNHc; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Metal-binding; Protein transport;
KW Reference proteome; Transport; Virulence; Zinc.
FT CHAIN 1..214
FT /note="Type 4 apparatus protein DotN"
FT /id="PRO_0000455592"
FT BINDING 52
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="structural"
FT /evidence="ECO:0000269|PubMed:28714967"
FT BINDING 55
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="structural"
FT /evidence="ECO:0000269|PubMed:28714967"
FT BINDING 84
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="structural"
FT /evidence="ECO:0000269|PubMed:28714967"
FT BINDING 87
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="structural"
FT /evidence="ECO:0000269|PubMed:28714967"
FT MUTAGEN 200..214
FT /note="Missing: Decreases intracellular growth in
FT A.castellanii."
FT /evidence="ECO:0000269|PubMed:32513920"
SQ SEQUENCE 214 AA; 24424 MW; 451736AC980BDF10 CRC64;
MNVNQTMADN QQRCELKLIA SPGSWRLYSA RKIDERFKSY EQKIFQRDRY TCQFCGFQAR
LYQDIVNLDG DYTNNRLSNL VTACCFCAQC FFVESVGVGG YGGGTLIYLP ELTQAELNSL
CHVLFCAITN DTGYKSSAQN IYRSFKFRSQ IVEEKFGEGT SDPAIFGQLM IDSGVNSEEI
REKLFKNIRL LPSRAKFRKQ IEKWAASALE EIAD
