DOTY_LEGPH
ID DOTY_LEGPH Reviewed; 230 AA.
AC Q5ZYR7;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Type 4 apparatus protein DotY {ECO:0000305};
GN Name=dotY {ECO:0000303|PubMed:32513920};
GN OrderedLocusNames=lpg0294 {ECO:0000312|EMBL:AAU26401.1};
OS Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC
OS 33152 / DSM 7513).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
OC Legionellaceae; Legionella.
OX NCBI_TaxID=272624;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Philadelphia 1 / ATCC 33152 / DSM 7513;
RX PubMed=15448271; DOI=10.1126/science.1099776;
RA Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G.,
RA Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V.,
RA Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S., Georghiou A.,
RA Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A.,
RA Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A.,
RA Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A.,
RA Russo J.J.;
RT "The genomic sequence of the accidental pathogen Legionella pneumophila.";
RL Science 305:1966-1968(2004).
RN [2]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Philadelphia 1 / Lp01;
RX PubMed=34816517; DOI=10.1111/mmi.14847;
RA Mace K., Meir A., Lukoyanova N., Liu L., Chetrit D., Hospenthal M.K.,
RA Roy C.R., Waksman G.;
RT "Proteins DotY and DotZ modulate the dynamics and localization of the type
RT IVB coupling complex of Legionella pneumophila.";
RL Mol. Microbiol. 117:307-319(2022).
RN [3] {ECO:0007744|PDB:6SZ9}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.70 ANGSTROMS), FUNCTION, SUBUNIT, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=Philadelphia 1 / Lp01;
RX PubMed=32513920; DOI=10.1038/s41467-020-16681-z;
RA Meir A., Mace K., Lukoyanova N., Chetrit D., Hospenthal M.K., Redzej A.,
RA Roy C., Waksman G.;
RT "Mechanism of effector capture and delivery by the type IV secretion system
RT from Legionella pneumophila.";
RL Nat. Commun. 11:2864-2864(2020).
CC -!- FUNCTION: Component of the Dot/Icm type IVB secretion system (T4BSS),
CC which is used to inject bacterial effector proteins into eukaryotic
CC host cells (PubMed:32513920). Part of a subcomplex which recruits
CC effector proteins and delivers them to the core transmembrane
CC subcomplex (PubMed:32513920). DotY and DotZ play a role in effector
CC translocation, but are not essential and do not influence the stability
CC of the subcomplex main components (PubMed:34816517). The DotY/DotZ main
CC function is to optimize secretion by modulating the delivery trajectory
CC of the IcmSW module and the localization of the machinery to the poles
CC (PubMed:34816517). {ECO:0000269|PubMed:32513920,
CC ECO:0000269|PubMed:34816517}.
CC -!- SUBUNIT: The T4BSS is a complex nanomachine composed of several
CC subcomplexes. This subunit is part of the Type IV Coupling Complex
CC (T4CC), a subcomplex composed of the DotLMNYZ core and the IcmSW-LvgA
CC adapter subunits, linked by the C-terminal tail of DotL
CC (PubMed:32513920). Six DotLMNYZ hetero-pentameric units may assemble
CC into an hexameric nanomachine, forming an inner membrane channel for
CC effectors to pass through (PubMed:32513920). Interacts exclusively with
CC DotZ (PubMed:32513920, PubMed:34816517). DotY and DotZ are co-dependent
CC for the assembly into the T4CC (PubMed:34816517).
CC {ECO:0000269|PubMed:32513920, ECO:0000269|PubMed:34816517}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:34816517}.
CC Note=Localizes to the poles of the cell. Localization depends on the
CC fully assembled T4CC subcomplex. {ECO:0000269|PubMed:34816517}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene leads to a reduced
CC intracellular growth in the protozoa A.castellanii and decreased levels
CC of effector translocation (PubMed:32513920). Deletion of dotY results
CC in the absence of both DotY and DotZ proteins in the coupling complex
CC (PubMed:34816517). Deletion mutant shows significantly reduced DotL
CC polar localization (PubMed:34816517). {ECO:0000269|PubMed:32513920,
CC ECO:0000269|PubMed:34816517}.
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DR EMBL; AE017354; AAU26401.1; -; Genomic_DNA.
DR RefSeq; WP_010946055.1; NC_002942.5.
DR RefSeq; YP_094348.1; NC_002942.5.
DR PDB; 6SZ9; EM; 3.70 A; E=1-230.
DR PDB; 7OVB; EM; 3.61 A; E=1-230.
DR PDBsum; 6SZ9; -.
DR PDBsum; 7OVB; -.
DR SMR; Q5ZYR7; -.
DR STRING; 272624.lpg0294; -.
DR PaxDb; Q5ZYR7; -.
DR PRIDE; Q5ZYR7; -.
DR EnsemblBacteria; AAU26401; AAU26401; lpg0294.
DR GeneID; 66489497; -.
DR KEGG; lpn:lpg0294; -.
DR PATRIC; fig|272624.6.peg.314; -.
DR eggNOG; ENOG5030NFC; Bacteria.
DR HOGENOM; CLU_093805_0_0_6; -.
DR OMA; ERPTSDY; -.
DR Proteomes; UP000000609; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Protein transport; Reference proteome; Transport;
KW Virulence.
FT CHAIN 1..230
FT /note="Type 4 apparatus protein DotY"
FT /id="PRO_0000455593"
FT REGION 202..230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..218
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 230 AA; 25520 MW; 3C02222476F1E912 CRC64;
MPKYTLPTRD ALLKAMQVGE TSIEAAEYMA TRFEQILTKA KLLPECNDML EKIKEYAQFV
KFKLLSSAQV WSGQERPTSD YQNTQENKAE FLASHLEGLP SGLKLEVAIG DDAKILRGFS
SNGKMVEGDQ LKTMDGLLEG WLAKNSLAIS GGAVVKIDNT GNQTKVDPQE IRQLINDSEK
GVAKYFADKG VGMEVAQRTY QEPKALETKR EEIRQEIESG AEAPTTQSIR
