DOTZ_LEGPH
ID DOTZ_LEGPH Reviewed; 294 AA.
AC Q5ZV91;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Type 4 apparatus protein DotZ {ECO:0000305};
GN Name=dotZ {ECO:0000303|PubMed:32513920};
GN OrderedLocusNames=lpg1549 {ECO:0000312|EMBL:AAU27631.1};
OS Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC
OS 33152 / DSM 7513).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
OC Legionellaceae; Legionella.
OX NCBI_TaxID=272624;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Philadelphia 1 / ATCC 33152 / DSM 7513;
RX PubMed=15448271; DOI=10.1126/science.1099776;
RA Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G.,
RA Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V.,
RA Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S., Georghiou A.,
RA Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A.,
RA Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A.,
RA Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A.,
RA Russo J.J.;
RT "The genomic sequence of the accidental pathogen Legionella pneumophila.";
RL Science 305:1966-1968(2004).
RN [2]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Philadelphia 1 / Lp01;
RX PubMed=34816517; DOI=10.1111/mmi.14847;
RA Mace K., Meir A., Lukoyanova N., Liu L., Chetrit D., Hospenthal M.K.,
RA Roy C.R., Waksman G.;
RT "Proteins DotY and DotZ modulate the dynamics and localization of the type
RT IVB coupling complex of Legionella pneumophila.";
RL Mol. Microbiol. 117:307-319(2022).
RN [3] {ECO:0007744|PDB:6SZ9}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.70 ANGSTROMS), FUNCTION, SUBUNIT,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 283-GLU--ARG-294.
RC STRAIN=Philadelphia 1 / Lp01;
RX PubMed=32513920; DOI=10.1038/s41467-020-16681-z;
RA Meir A., Mace K., Lukoyanova N., Chetrit D., Hospenthal M.K., Redzej A.,
RA Roy C., Waksman G.;
RT "Mechanism of effector capture and delivery by the type IV secretion system
RT from Legionella pneumophila.";
RL Nat. Commun. 11:2864-2864(2020).
CC -!- FUNCTION: Component of the Dot/Icm type IVB secretion system (T4BSS),
CC which is used to inject bacterial effector proteins into eukaryotic
CC host cells (PubMed:32513920). Part of a subcomplex which recruits
CC effector proteins and delivers them to the core transmembrane
CC subcomplex (PubMed:32513920). DotY and DotZ play a role in effector
CC translocation, but are not essential and do not influence the stability
CC of the subcomplex main components (PubMed:34816517). The DotY/DotZ main
CC function is to optimize secretion by modulating the delivery trajectory
CC of the IcmSW module and the localization of the machinery to the poles
CC (PubMed:34816517). {ECO:0000269|PubMed:32513920,
CC ECO:0000269|PubMed:34816517}.
CC -!- SUBUNIT: The T4BSS is a complex nanomachine composed of several
CC subcomplexes. This subunit is part of the Type IV Coupling Complex
CC (T4CC), a subcomplex composed of the DotLMNYZ core and the IcmSW-LvgA
CC adapter subunits, linked by the C-terminal tail of DotL
CC (PubMed:32513920). Six DotLMNYZ hetero-pentameric units may assemble
CC into an hexameric nanomachine, forming an inner membrane channel for
CC effectors to pass through (PubMed:32513920). Makes significant contact
CC with DotN and DotY, but engages weakly with DotM and DotL
CC (PubMed:32513920, PubMed:34816517). DotY and DotZ are co-dependent for
CC the assembly into the T4CC (PubMed:34816517).
CC {ECO:0000269|PubMed:32513920, ECO:0000269|PubMed:34816517}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:34816517}.
CC Note=Localizes to the poles of the cell. Localization depends on the
CC fully assembled T4CC subcomplex. {ECO:0000269|PubMed:34816517}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene leads to a reduced
CC intracellular growth in the protozoa A.castellanii and decreased levels
CC of effector translocation (PubMed:32513920). Deletion of dotZ results
CC in the absence of both DotY and DotZ proteins in the coupling complex
CC (PubMed:34816517). Deletion mutant shows significantly reduced DotL
CC polar localization (PubMed:34816517). {ECO:0000269|PubMed:32513920,
CC ECO:0000269|PubMed:34816517}.
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DR EMBL; AE017354; AAU27631.1; -; Genomic_DNA.
DR RefSeq; WP_010947278.1; NC_002942.5.
DR RefSeq; YP_095578.1; NC_002942.5.
DR PDB; 6SZ9; EM; 3.70 A; D=1-294.
DR PDB; 7OVB; EM; 3.61 A; D=1-294.
DR PDBsum; 6SZ9; -.
DR PDBsum; 7OVB; -.
DR SMR; Q5ZV91; -.
DR STRING; 272624.lpg1549; -.
DR PaxDb; Q5ZV91; -.
DR EnsemblBacteria; AAU27631; AAU27631; lpg1549.
DR GeneID; 66490679; -.
DR KEGG; lpn:lpg1549; -.
DR PATRIC; fig|272624.6.peg.1622; -.
DR eggNOG; ENOG5030SXU; Bacteria.
DR HOGENOM; CLU_945923_0_0_6; -.
DR OMA; WNTKLET; -.
DR Proteomes; UP000000609; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Protein transport; Reference proteome; Transport;
KW Virulence.
FT CHAIN 1..294
FT /note="Type 4 apparatus protein DotZ"
FT /id="PRO_0000455594"
FT MUTAGEN 283..294
FT /note="Missing: Decreases intracellular growth in
FT A.castellanii."
FT /evidence="ECO:0000269|PubMed:32513920"
SQ SEQUENCE 294 AA; 33846 MW; 2E5A0B326FCB8525 CRC64;
MDEIKKDDEL SQWLSTYGTI TAERILGRYN ISLPQDEILE AINIPSSFYR HLLQIPLKNV
LNGIVIQQAS DYHVYAQKLL IDYLLSGESS KEPDSQGAGT RESLEDERQR LVQLGDEFHK
LELEQDNLIA SSQASLMKIS IDWNTKLETT LSKLNSLYKN TNSKIKKNAI RKALIKAFIH
CDLVKDQSQK NKYQLIDKLN QTLAVSVGAE LKESILTNLS ELFQILEALN TKLDEFTDRT
NHLSQQAKSF RTQFYEVILR IIELIKLLPE YKIDPAQDAI NREPLYFDRT IGER
