ID   DP2L_CENSY              Reviewed;        1112 AA.
AC   A0RYM0;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=DNA polymerase II large subunit {ECO:0000255|HAMAP-Rule:MF_00324};
DE            Short=Pol II {ECO:0000255|HAMAP-Rule:MF_00324};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00324};
DE   AltName: Full=Exodeoxyribonuclease large subunit {ECO:0000255|HAMAP-Rule:MF_00324};
DE            EC=3.1.11.1 {ECO:0000255|HAMAP-Rule:MF_00324};
GN   Name=polC {ECO:0000255|HAMAP-Rule:MF_00324}; OrderedLocusNames=CENSYa_1827;
OS   Cenarchaeum symbiosum (strain A).
OC   Archaea; Thaumarchaeota; Cenarchaeales; Cenarchaeaceae; Cenarchaeum.
OX   NCBI_TaxID=414004;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A;
RX   PubMed=17114289; DOI=10.1073/pnas.0608549103;
RA   Hallam S.J., Konstantinidis K.T., Putnam N., Schleper C., Watanabe Y.,
RA   Sugahara J., Preston C., de la Torre J., Richardson P.M., DeLong E.F.;
RT   "Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum
RT   symbiosum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:18296-18301(2006).
CC   -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an
CC       exonucleolytic activity that degrades single-stranded DNA in the 3'- to
CC       5'-direction. Has a template-primer preference which is characteristic
CC       of a replicative DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00324};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.11.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00324};
CC   -!- SUBUNIT: Heterodimer of a large subunit and a small subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_00324}.
CC   -!- SIMILARITY: Belongs to the archaeal DNA polymerase II family.
CC       {ECO:0000255|HAMAP-Rule:MF_00324}.
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DR   EMBL; DP000238; ABK78437.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0RYM0; -.
DR   SMR; A0RYM0; -.
DR   STRING; 414004.CENSYa_1827; -.
DR   PRIDE; A0RYM0; -.
DR   EnsemblBacteria; ABK78437; ABK78437; CENSYa_1827.
DR   KEGG; csy:CENSYa_1827; -.
DR   PATRIC; fig|414004.10.peg.1669; -.
DR   HOGENOM; CLU_001154_0_0_2; -.
DR   OMA; KRRNCDG; -.
DR   Proteomes; UP000000758; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008852; F:exodeoxyribonuclease I activity; IEA:UniProtKB-EC.
DR   GO; GO:0000738; P:DNA catabolic process, exonucleolytic; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00324; DNApol_II_L_arch; 1.
DR   InterPro; IPR004475; PolC_DP2.
DR   InterPro; IPR016033; PolC_DP2_N.
DR   PANTHER; PTHR42210; PTHR42210; 1.
DR   Pfam; PF03833; PolC_DP2; 1.
DR   PIRSF; PIRSF016275; PolC_DP2; 1.
DR   TIGRFAMs; TIGR00354; polC; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease;
KW   Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase;
KW   Reference proteome; Transferase.
FT   CHAIN           1..1112
FT                   /note="DNA polymerase II large subunit"
FT                   /id="PRO_0000294676"
SQ   SEQUENCE   1112 AA;  121039 MW;  BA05392302C4ECF4 CRC64;
     MSGGDVEEYL RGADMPEEYR RYYSSLSGGT YDIFEKAASA KSNLADSSGM VEPKIAVDLS
     DRVAKMHDLD IAGPLRELLA TKGKELAALM LAKEIMDGKY LPEADIEKRI DSAVRVGLAV
     VTEGVTIAPL QGIADVITKK NKDGSEYLSV SIAGPMRSAG GTESAVTMLI ADYVRRQAGL
     AEYQADSLDD ETGRFIEELR IYEREVGSFQ FHVLDEDIRA VISHLPVELD GVDTDPYEVV
     NHKGMSRIQT DRVRGGALRV LNDGLIGRSK KLLKRIELYG LDGWEWLAEL KGAVQTGENK
     EDAAAKRMRE VITGRSVLSM PNRLGGFRLR YGRACNTGYT SVGFHPAVAE ILDHTIAVGT
     QVKIDIPGKG ATVAFVDTIE APTVRLAGGD VVKIRDVAHG IELKGSIERI LHLGDMLISF
     GDFLENNAQL VPSGYVEEIW KMDMEAAGAA QGSPSSADEA VRISRELGVP LHPRYLYYWD
     QISHEELAML LSPLDKGDAI SYPAACKPVL EKLGVPHKAG PEGPVLEGDE ARIFRELILD
     NPPGPDASAP VPELISRSSG ITIRDKFSTS IGVRIGRPEK AAPRQMRPPT HCLFPVGGTG
     GPTNNLLKSA ARPGFSADIL SRRCPGCGEP SISIRCWACG ERTAVERTCM QCGTDVDGEE
     CERCGRPGLA HSRVEFPLKK MLVSAQEKTG VRAHDPLKGV KELAHQDRIA EPLEKGLIRQ
     SRSLTVFKDG TVRFDATNSP MTHFKPSWIG TSAEKLRELG YETDVDGKKL EGPDQLVELR
     MQDIVIPLEG AKYLVSACGY IDAELDKLYG APPFYKVPDL GGLIGHLVVG LAPHTSVGVA
     ARIIGYTETH VCFGTPNWHS AKRRDADGDA DSIILLMDAL LNFSRHYLSD RIGGLMDAPL
     LIQPLVLPHE SQSQAHNIEV VKRLPPGFYE AAAARKKASE VGCVEIVKSR LETAGQFGGY
     HFTHGTSSLT TSRPRSAYST LGSMLDKLDL QIRNANLIAA VDAAEIISNV ISTHLVPDIM
     GNLRAYARQN FRCTACGKSY RRIPLAQRCS CGNGLIQTIT RASVEKYLKL AKRLVNEYDV
     GAYQRGRIHA LSDEIELVFG KGGGDQALLT DF