DP2L_METBU
ID DP2L_METBU Reviewed; 1145 AA.
AC Q12TF2;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=DNA polymerase II large subunit {ECO:0000255|HAMAP-Rule:MF_00324};
DE Short=Pol II {ECO:0000255|HAMAP-Rule:MF_00324};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00324};
DE AltName: Full=Exodeoxyribonuclease large subunit {ECO:0000255|HAMAP-Rule:MF_00324};
DE EC=3.1.11.1 {ECO:0000255|HAMAP-Rule:MF_00324};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00324}; OrderedLocusNames=Mbur_2423;
OS Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 /
OS ACE-M).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanococcoides.
OX NCBI_TaxID=259564;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6242 / NBRC 107633 / OCM 468 / ACE-M;
RX PubMed=19404327; DOI=10.1038/ismej.2009.45;
RA Allen M.A., Lauro F.M., Williams T.J., Burg D., Siddiqui K.S.,
RA De Francisci D., Chong K.W., Pilak O., Chew H.H., De Maere M.Z., Ting L.,
RA Katrib M., Ng C., Sowers K.R., Galperin M.Y., Anderson I.J., Ivanova N.,
RA Dalin E., Martinez M., Lapidus A., Hauser L., Land M., Thomas T.,
RA Cavicchioli R.;
RT "The genome sequence of the psychrophilic archaeon, Methanococcoides
RT burtonii: the role of genome evolution in cold adaptation.";
RL ISME J. 3:1012-1035(2009).
CC -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an
CC exonucleolytic activity that degrades single-stranded DNA in the 3'- to
CC 5'-direction. Has a template-primer preference which is characteristic
CC of a replicative DNA polymerase (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00324};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC nucleoside 5'-phosphates.; EC=3.1.11.1; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00324};
CC -!- SUBUNIT: Heterodimer of a large subunit and a small subunit.
CC {ECO:0000255|HAMAP-Rule:MF_00324}.
CC -!- SIMILARITY: Belongs to the archaeal DNA polymerase II family.
CC {ECO:0000255|HAMAP-Rule:MF_00324}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABE53274.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000300; ABE53274.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_048063431.1; NC_007955.1.
DR AlphaFoldDB; Q12TF2; -.
DR SMR; Q12TF2; -.
DR STRING; 259564.Mbur_2423; -.
DR EnsemblBacteria; ABE53274; ABE53274; Mbur_2423.
DR GeneID; 3999013; -.
DR KEGG; mbu:Mbur_2423; -.
DR HOGENOM; CLU_001154_0_0_2; -.
DR OrthoDB; 559at2157; -.
DR Proteomes; UP000001979; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008852; F:exodeoxyribonuclease I activity; IEA:UniProtKB-EC.
DR GO; GO:0000738; P:DNA catabolic process, exonucleolytic; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00324; DNApol_II_L_arch; 1.
DR InterPro; IPR004475; PolC_DP2.
DR InterPro; IPR016033; PolC_DP2_N.
DR PANTHER; PTHR42210; PTHR42210; 1.
DR Pfam; PF03833; PolC_DP2; 1.
DR PIRSF; PIRSF016275; PolC_DP2; 1.
DR TIGRFAMs; TIGR00354; polC; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase;
KW Reference proteome; Transferase.
FT CHAIN 1..1145
FT /note="DNA polymerase II large subunit"
FT /id="PRO_0000294685"
FT REGION 284..303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 286..303
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1145 AA; 127582 MW; 2E4E696ABF9D18E4 CRC64;
MAEIVASDEM NEYFNTLEGT LKKEIDIVND ARSRGKDPKP HVEIPLAKDL ADRVENLIGV
KGVAELIRKL EETMSREEAA LALGREVAQG KVGEFDSKSE AIEAAIRVSV AMLTEGVVAA
PIEGIDRASI GKNDDGSEYV SIFYAGPIRS AGGTAQALSV LVGDYVRRGV GIDRYKPRKE
EVERYIEEIM LYKRVASLQY TPSEEEIRLI VENCPICIDG EPTEAEEVEG HRNLERIDTN
RVRGGMALVL AEGLALKAPK IQKHVKNLKI DGWEWLEQLI SGVKSSSESD EDEETDGKPK
IKPKDKYMRD LIAGRPVFSH PSRPGGFRLR YGRSRNTSFA AAGISPAGMI VMDDFIAPGT
QLKVERPGKA AGMAPVDSIE GPTVRLNNGD VIRIDTIDEA YALRSEVEEI IDIGEILINY
GDFLENNHPL APSPYCFEWW IQEYRKAAPD TETNEAELKE PTQEVALDLC KELNIPLHPK
FTYLWHDIDN SQYTALADLI SKDGLLEADG SFLKLPLQRT IDTGMKKVLE DLLVQHKIQG
QALTIEEPLP LIHSLGLDEE LSKGWDSLGH EELLENINEI AGFVVRPRAP TRIGARMGRP
EKSDKRKMTP APHALFPIAE AGGNTRSLEK AANFKVNTNS KAGTIPVEIG NRICPACGVE
GFEFRCECGE YTLPKLFCPR CGISVNKEKC PKCNSKTTCT SMRKIDFKSI YQKAFESIGE
RDHLDSFKGV KKMMSKHMTP EPLEKGILRA KHGLFTFKDG TVRYDMSDIP LTHIRPAEIG
VSCERMIELG YLKDIYGKPL IDSEQVLCLK VQDLVISYDA ADYILRITQY IDDLLVKYYK
VAPYYNAKHI DDIVGVLLMG LAPHTSAGVL GRLIGFTTAS VGYAHPYFHA AKRRNCDGDE
DCVMLLMDGL LNFSRDYLPD KRGGQMDAPL VLTTRLDPSE VDKEAHNIDM CASYPLEFYE
ATQNIANPKD FEGTMDLVSG RLGTTLQYEE FMFTHDTSNI AAGPLKSAYK TLGTMVEKMD
AQLELAKKIR AVDAPDVAER VLTSHFLPDM FGNLRAFSRQ RTRCVKCAAK FRRPPLTGSC
PKCGGRVILT VHEGAVKKYL QVSIKIAEEY NVSSYTKQRI ELIGYDMKSL FENDKSKQMG
LSDFM
