ID   DP2L_METKA              Reviewed;        1240 AA.
AC   Q8TUV3;
DT   19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=DNA polymerase II large subunit {ECO:0000255|HAMAP-Rule:MF_00324};
DE            Short=Pol II {ECO:0000255|HAMAP-Rule:MF_00324};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00324};
DE   AltName: Full=Exodeoxyribonuclease large subunit {ECO:0000255|HAMAP-Rule:MF_00324};
DE            EC=3.1.11.1 {ECO:0000255|HAMAP-Rule:MF_00324};
GN   Name=polC {ECO:0000255|HAMAP-Rule:MF_00324}; OrderedLocusNames=MK1650;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an
CC       exonucleolytic activity that degrades single-stranded DNA in the 3'- to
CC       5'-direction. Has a template-primer preference which is characteristic
CC       of a replicative DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00324};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.11.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00324};
CC   -!- SUBUNIT: Heterodimer of a large subunit and a small subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_00324}.
CC   -!- SIMILARITY: Belongs to the archaeal DNA polymerase II family.
CC       {ECO:0000255|HAMAP-Rule:MF_00324}.
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DR   EMBL; AE009439; AAM02863.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8TUV3; -.
DR   SMR; Q8TUV3; -.
DR   STRING; 190192.MK1650; -.
DR   EnsemblBacteria; AAM02863; AAM02863; MK1650.
DR   KEGG; mka:MK1650; -.
DR   PATRIC; fig|190192.8.peg.1813; -.
DR   HOGENOM; CLU_001154_0_0_2; -.
DR   OMA; KRRNCDG; -.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008852; F:exodeoxyribonuclease I activity; IEA:UniProtKB-EC.
DR   GO; GO:0000738; P:DNA catabolic process, exonucleolytic; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00324; DNApol_II_L_arch; 1.
DR   InterPro; IPR004475; PolC_DP2.
DR   InterPro; IPR016033; PolC_DP2_N.
DR   PANTHER; PTHR42210; PTHR42210; 2.
DR   Pfam; PF03833; PolC_DP2; 2.
DR   PIRSF; PIRSF016275; PolC_DP2; 1.
DR   TIGRFAMs; TIGR00354; polC; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease;
KW   Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase;
KW   Reference proteome; Transferase.
FT   CHAIN           1..1240
FT                   /note="DNA polymerase II large subunit"
FT                   /id="PRO_0000152576"
SQ   SEQUENCE   1240 AA;  140145 MW;  4BEEE6117BCDD9AD CRC64;
     MNRAREELDR YRETLEEVSS RFVDVATKAR QRREDPKPEP EVMLATSIGE RVEGLLQVEN
     VADRLEELEE ELGDREEATF RIVEEVIKGE LKVKGDLPLH KRIDYAVRIG LAVLTEAVVS
     APLEGIAAVE IRERGTGHRV VDESEPPHEE PKLVCTECGK EVDPENCYLA VKYAGPIRAA
     GGTAAALSAL LADYARQVAG LPRFNPDDFD HDLVGRYVEE VVTYLDKVGS FQYNPSEEEV
     ELVAKNIPIE IDGEPTEEVE VQGHRDIPHL PNQLRGGALL VICEGICQKA PKLIKRVEKY
     GIDGWEFLEK LVNKGSDDEE EGEEEKTKIK PNDKYMGELV AGRPLLSHPS AKGGFRLRYG
     RARNTGFAAV GVHPSLMYVT KGFIVIGTQL KVERPGKAAC VLPVTEIEPP VVKLRDGSVV
     RLDDPREAKE LVEKDEIEEI LDLGEMLVAV GEFIENNHPL VPPAYCPEWW VKEVPDVVKV
     IGLRKNLPND VFEKLKDVPL KRLVKEASRL SGNGNNLDGF LNGPVKVARS IVELVRKEVI
     PRLSSPERMS VEEAIELSRE YGVPFHPKYT FLWHDVKPKD VDELREALEV AGSEWGNLRV
     EFENDGEIKR ILEDLLVPHR LEDDTIVVEE PWASALLAQL GYDPESGEFR EQDEDLDYLL
     DYLVIRDETC RYVSKLAGFP IREKAPTRIG ARMGRPEKAR ERKMSPPPHV LFPIGIAGGN
     QRDIMKFHRG ESEDTDRVEV CYRICPECDR LVPYRVCPFC GTETVQYCNR CDEPADECDC
     EEPDPVVRAD IERNDDPYSS LPVRELVRRA EEEVGTTDTL KGVKGMTSRL KMPEPLQKGI
     LRAKRDLFVF KDGTLRFDCN DCPLTHVRLK EVGLTPFKAR LLGFERDING DPVVSEDQVV
     ELYPQDVVLP RKAAEWAVRV CQYLDDLLRK YYGLEPVYGV EKPEDLIGHL IVTLAPHTSC
     GVVGRVVGIA DINCWYNHPI INAARRRNCD GDEDAFMLLL DVLLNFSRLY LPDKRGGLMD
     APLVLTAVVD PYEIDDEVWN MDVCGDYPLE LYRKALEYAD AGEAEELIER LEDRLDLPRG
     LQFTHDTEAI DLGPTVTRYS RLEKMEEKLE EQLDLARRIR AVDESDVAKI VLDSHFLPDI
     KGNLRKFGRQ KFRCSRCNAK FNVPPLSGKC PRCGGDVLLT IYPATATKYL EPAKRLVEEF
     GTHDEEWRTI ASEVELLEEE AKTLFGSDHS VSLKKFFGET