ID   DP2L_METLZ              Reviewed;        1141 AA.
AC   A2SU54;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=DNA polymerase II large subunit {ECO:0000255|HAMAP-Rule:MF_00324};
DE            Short=Pol II {ECO:0000255|HAMAP-Rule:MF_00324};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00324};
DE   AltName: Full=Exodeoxyribonuclease large subunit {ECO:0000255|HAMAP-Rule:MF_00324};
DE            EC=3.1.11.1 {ECO:0000255|HAMAP-Rule:MF_00324};
GN   Name=polC {ECO:0000255|HAMAP-Rule:MF_00324}; OrderedLocusNames=Mlab_1699;
OS   Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
OX   NCBI_TaxID=410358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43576 / DSM 4855 / Z;
RX   PubMed=21304657; DOI=10.4056/sigs.35575;
RA   Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A.,
RA   Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L.,
RA   Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.;
RT   "Complete genome sequence of Methanocorpusculum labreanum type strain Z.";
RL   Stand. Genomic Sci. 1:197-203(2009).
CC   -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an
CC       exonucleolytic activity that degrades single-stranded DNA in the 3'- to
CC       5'-direction. Has a template-primer preference which is characteristic
CC       of a replicative DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00324};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.11.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00324};
CC   -!- SUBUNIT: Heterodimer of a large subunit and a small subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_00324}.
CC   -!- SIMILARITY: Belongs to the archaeal DNA polymerase II family.
CC       {ECO:0000255|HAMAP-Rule:MF_00324}.
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DR   EMBL; CP000559; ABN07860.1; -; Genomic_DNA.
DR   RefSeq; WP_011834063.1; NC_008942.1.
DR   AlphaFoldDB; A2SU54; -.
DR   SMR; A2SU54; -.
DR   STRING; 410358.Mlab_1699; -.
DR   EnsemblBacteria; ABN07860; ABN07860; Mlab_1699.
DR   GeneID; 4795011; -.
DR   KEGG; mla:Mlab_1699; -.
DR   eggNOG; arCOG04447; Archaea.
DR   HOGENOM; CLU_001154_0_0_2; -.
DR   OMA; KRRNCDG; -.
DR   OrthoDB; 559at2157; -.
DR   Proteomes; UP000000365; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008852; F:exodeoxyribonuclease I activity; IEA:UniProtKB-EC.
DR   GO; GO:0000738; P:DNA catabolic process, exonucleolytic; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00324; DNApol_II_L_arch; 1.
DR   InterPro; IPR004475; PolC_DP2.
DR   InterPro; IPR016033; PolC_DP2_N.
DR   PANTHER; PTHR42210; PTHR42210; 1.
DR   Pfam; PF03833; PolC_DP2; 1.
DR   PIRSF; PIRSF016275; PolC_DP2; 1.
DR   TIGRFAMs; TIGR00354; polC; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease;
KW   Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase;
KW   Reference proteome; Transferase.
FT   CHAIN           1..1141
FT                   /note="DNA polymerase II large subunit"
FT                   /id="PRO_0000294687"
FT   REGION          567..587
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1141 AA;  126420 MW;  E52E99960EC631DD CRC64;
     MAELVTSPAY RAYLDGLIAG LDRAMEIANK AKSLGVDPRP YVEIPIASDL AGRVEALLGY
     KGVAAKIREL EDRMSREEAA LKIGDAFVGK EFGESTREDI LDHAIRTSMA LLTEGVVAAP
     TEGIGKVAVR RNDDGTEYLS IYYAGPIRSA GGTAQALSVL VGDYVRRLLN IDRYKPREEE
     IERYVEEIKQ YNNIQSMQYL PKDDDIRLII RNCPICIDGE PTEQEEISGY RNLERVETNV
     VRGGMGLVIA EGIGLKAPKI QKNVAKMNLD GWEWLEELIS GNTPAQEVEE EPGVHPKDKY
     MRDMLAGRPS FSYPMRKGGF RFRLGRCRNT GLATCGFNPA TLHILDDYLA VGTQMKVERP
     GKACGVVPCT DIEGPTVRLK SGELRRIDTL DDANKYYDQV EYILDIGEIL ISFGEFMENN
     HVLMPPSYCE EWWIQEGGPR HPKNEAEALS FVLEGAYLHP DYTWFWDDCS EGQLIFLSDK
     VAGTGSLREG VLYIPEDPAV KSVLEELLVP HTVEEGFYVV RTPLALIMGL GLTDTLAKSP
     TWKTLPPFSN GLSMAISLSG LKMRSKAGTR VGGRMGRPGK SAPRKMKPPV HVLFPIGESG
     GMKRSIDNAA KICSSDSEEN FRGTSVTTGQ VEGLIQVQTG ERRCPKCGTV TFKSRCADCG
     THTDAVYRCP HCNQLGEEGQ ESCPKCGANL VCSKESIVSL GQEYAAALKN VGMSASSSPE
     LKGVRGLISR ERVVEPLEKG LLRAKNNIFV FRDGTIRYDM IDLPLTHFRP AEVGVSVEKL
     RSIGYTQDMH GADLTDASQL VELHPQDIMV SVDCGEYLVR VAAYVDELLE KVYGMEAFYH
     VKTPEDLVGH LVMGLAPHTS AGVLARIVGF TKAKAGYAHP YYHAAKRRNC DGDEDCVMLL
     MDGLLNFSRS FLPSTRGGTM DAPLVLTTTL NPKEVDKETL NVDVMPRYPL EVYTACLTYR
     APKEVGKFVD YVEKRVETPG QFEGFSFTHD TTDISEGPID TMYTNPILKG TADKIKAELG
     LADRIRAVDT NDLAERIINS HLMPDMIGNL RSFSKQAFRC PKCKTSYRRI PVSGKCNKCG
     GPVKATMHKG NVTKYLEISK YMAEHYTLSD YTNQRIQVTE MNINSTFGEE EKVQMDLSDF
     F