ID   DP2S_METTH              Reviewed;         482 AA.
AC   O27456;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=DNA polymerase II small subunit;
DE            Short=Pol II;
DE            EC=2.7.7.7;
DE   AltName: Full=Exodeoxyribonuclease small subunit;
DE            EC=3.1.11.1;
GN   Name=polB; OrderedLocusNames=MTH_1405;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an
CC       exonucleolytic activity that degrades single-stranded DNA in the 3' to
CC       5' direction. Has a template-primer preference which is characteristic
CC       of a replicative DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.11.1;
CC   -!- SUBUNIT: Heterodimer of a large subunit and a small subunit.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase delta/II small subunit
CC       family. {ECO:0000305}.
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DR   EMBL; AE000666; AAB85882.1; -; Genomic_DNA.
DR   PIR; B69054; B69054.
DR   RefSeq; WP_010877017.1; NC_000916.1.
DR   AlphaFoldDB; O27456; -.
DR   SMR; O27456; -.
DR   STRING; 187420.MTH_1405; -.
DR   PRIDE; O27456; -.
DR   EnsemblBacteria; AAB85882; AAB85882; MTH_1405.
DR   GeneID; 24854519; -.
DR   KEGG; mth:MTH_1405; -.
DR   PATRIC; fig|187420.15.peg.1370; -.
DR   HOGENOM; CLU_027850_1_0_2; -.
DR   OMA; HVQAALI; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008852; F:exodeoxyribonuclease I activity; IEA:UniProtKB-EC.
DR   GO; GO:0000738; P:DNA catabolic process, exonucleolytic; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00325; DNApol_II_A_arch; 1.
DR   InterPro; IPR007185; DNA_pol_a/d/e_bsu.
DR   InterPro; IPR024826; DNA_pol_delta/II_ssu.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR011149; Pol2_small_arc.
DR   PANTHER; PTHR10416; PTHR10416; 1.
DR   Pfam; PF04042; DNA_pol_E_B; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   PIRSF; PIRSF000803; Arc_Pol2_small; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF56300; SSF56300; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease;
KW   Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase;
KW   Reference proteome; Transferase.
FT   CHAIN           1..482
FT                   /note="DNA polymerase II small subunit"
FT                   /id="PRO_0000096178"
SQ   SEQUENCE   482 AA;  54376 MW;  8E3262AD7579F2C5 CRC64;
     MNEIIGKFAR EGILIEDNAY FRLREMDDPA SVSSELIVKI KSNGGKFTVL TSEMLDEFFE
     IDNPAEIKAR GPLMVPAERD FDFEVISDTS NRSYTSGEIG DMIAYFNSRY SSLKNLLSKR
     PELKGHIPIA DLRGGEDVVS IIGMVNDVRN TKNNHRIIEL EDDTGEISVV VHNENHKLFE
     KSEKIVRDEV VGVHGTKKGR FVVASEIFHP GVPRIQEKEM DFSVAFISDV HIGSQTFLED
     AFMKFVKWIN GDFGSEEQRS LAADVKYLVV AGDIVDGIGI YPGQEKELLI RDIHEQYEEA
     ARLFGDIRSD IKIVMIPGNH DSSRIAEPQP AIPEEYAKSL YSIRNIEFLS NPSLVSLDGV
     RTLIYHGRSF DDMAMSVNGL SHERSDLIME ELLEKRHLAP IYGERTPLAS EIEDHLVIDE
     VPHVLHTGHV HINAYKKYKG VHLINSGTFQ SQTEFQKIYN IVPTCGQVPV LNRGVMKLLE
     FS