ID   DP2S_THEAC              Reviewed;         488 AA.
AC   Q9HLK5;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=DNA polymerase II small subunit;
DE            Short=Pol II;
DE            EC=2.7.7.7;
DE   AltName: Full=Exodeoxyribonuclease small subunit;
DE            EC=3.1.11.1;
GN   Name=polB; OrderedLocusNames=Ta0222;
OS   Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC
OS   15155 / AMRC-C165).
OC   Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC   Thermoplasmataceae; Thermoplasma.
OX   NCBI_TaxID=273075;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165;
RX   PubMed=11029001; DOI=10.1038/35035069;
RA   Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C.,
RA   Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.;
RT   "The genome sequence of the thermoacidophilic scavenger Thermoplasma
RT   acidophilum.";
RL   Nature 407:508-513(2000).
CC   -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an
CC       exonucleolytic activity that degrades single-stranded DNA in the 3' to
CC       5' direction. Has a template-primer preference which is characteristic
CC       of a replicative DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.11.1;
CC   -!- SUBUNIT: Heterodimer of a large subunit and a small subunit.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase delta/II small subunit
CC       family. {ECO:0000305}.
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DR   EMBL; AL445063; CAC11368.1; -; Genomic_DNA.
DR   RefSeq; WP_010900649.1; NC_002578.1.
DR   AlphaFoldDB; Q9HLK5; -.
DR   SMR; Q9HLK5; -.
DR   STRING; 273075.Ta0222; -.
DR   DNASU; 1455858; -.
DR   EnsemblBacteria; CAC11368; CAC11368; CAC11368.
DR   GeneID; 1455858; -.
DR   KEGG; tac:Ta0222; -.
DR   eggNOG; arCOG04455; Archaea.
DR   HOGENOM; CLU_027850_1_0_2; -.
DR   OMA; HVQAALI; -.
DR   OrthoDB; 24499at2157; -.
DR   Proteomes; UP000001024; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008852; F:exodeoxyribonuclease I activity; IEA:UniProtKB-EC.
DR   GO; GO:0000738; P:DNA catabolic process, exonucleolytic; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00325; DNApol_II_A_arch; 1.
DR   InterPro; IPR007185; DNA_pol_a/d/e_bsu.
DR   InterPro; IPR024826; DNA_pol_delta/II_ssu.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   InterPro; IPR011149; Pol2_small_arc.
DR   PANTHER; PTHR10416; PTHR10416; 1.
DR   Pfam; PF04042; DNA_pol_E_B; 1.
DR   PIRSF; PIRSF000803; Arc_Pol2_small; 1.
DR   SUPFAM; SSF56300; SSF56300; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease;
KW   Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase;
KW   Reference proteome; Transferase.
FT   CHAIN           1..488
FT                   /note="DNA polymerase II small subunit"
FT                   /id="PRO_0000096182"
SQ   SEQUENCE   488 AA;  55577 MW;  3307E418E35AC27A CRC64;
     MVIWFFYGFR KIMHLSLKRI IVKCKMSQLD RTSLIQYFNS NGVLISPEAL DKLIKYSSSS
     LIEDLIKNSD GYIDEKYVER YVSRPKVRND YEVYLPDVRF NASIEDFRKM FVSKYEKLSK
     IITSSSSMRG SISIKTAKRS QGEVKVVGMV SEVSMTKNGH KRIVIEDLDD SITAIVMKDR
     GPVNEIILED EVIGIIGSVS QSSKDPVIFV NEIIRPDIPY RVIDEEKHEP VYVASISDIH
     VGSKTFRKNE FEAMVRWISG SDPDASRVKY LILSGDVVDG IGVYPDQEND LEILNPLEQY
     ANLAEYLVDV PEDVKVFVMP GNHDTVRLSE PQPVFPGKIR DLFEPNVAFL PNPYNLKLEG
     KNVLVYHGMS LNDMIELIPG ANYDSIGKAI EAILVRRHLS PKYGGNTPMI PSAVDYHVIE
     EVPDIFITGH IHSHYIGNYK GVRYVNSSTW QSQTEYQKMM NFNPKPSKLT LFDLYSRSVI
     VKDFDTTL