ID   DP71L_ASFB7             Reviewed;          71 AA.
AC   Q65212; O12410; O12411; O12412; O12413; O12414; O12415; O12706; O12707;
AC   O12708; O12709; O12710; O12711;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Protein DP71L;
DE   AltName: Full=MyD116 homolog;
GN   OrderedLocusNames=Ba71V-154; ORFNames=DP71L;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [2]
RP   INTERACTION WITH HOST PPP1CB.
RX   PubMed=17215279; DOI=10.1128/jvi.02077-06;
RA   Rivera J., Abrams C., Hernaez B., Alcazar A., Escribano J.M., Dixon L.,
RA   Alonso C.;
RT   "The MyD116 African swine fever virus homologue interacts with the
RT   catalytic subunit of protein phosphatase 1 and activates its phosphatase
RT   activity.";
RL   J. Virol. 81:2923-2929(2007).
RN   [3]
RP   FUNCTION.
RX   PubMed=20702639; DOI=10.1128/jvi.01027-10;
RA   Zhang F., Moon A., Childs K., Goodbourn S., Dixon L.K.;
RT   "The African swine fever virus DP71L protein recruits the protein
RT   phosphatase 1 catalytic subunit to dephosphorylate eIF2alpha and inhibits
RT   CHOP induction but is dispensable for these activities during virus
RT   infection.";
RL   J. Virol. 84:10681-10689(2010).
RN   [4]
RP   FUNCTION, AND MUTAGENESIS OF VAL-16; PHE-18; LEU-57 AND LEU-61.
RX   PubMed=28189088; DOI=10.1016/j.virol.2017.02.002;
RA   Barber C., Netherton C., Goatley L., Moon A., Goodbourn S., Dixon L.;
RT   "Identification of residues within the African swine fever virus DP71L
RT   protein required for dephosphorylation of translation initiation factor
RT   eIF2alpha and inhibiting activation of pro-apoptotic CHOP.";
RL   Virology 504:107-113(2017).
RN   [5]
RP   INDUCTION.
RX   PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA   Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA   Dixon L., Werner F.;
RT   "The African Swine Fever Virus Transcriptome.";
RL   J. Virol. 94:0-0(2020).
CC   -!- FUNCTION: Interacts with the host phosphatase PP1 catalytic subunit
CC       (PPP1CB) and recruits it to dephosphorylate EIF2S1/eIF2alpha and
CC       therefore restores the host translation that has been shut-down by the
CC       host. Also inhibits the EIF2S1/eIF2alpha-ATF4-DDIT3/CHOP pathway.
CC       {ECO:0000269|PubMed:20702639, ECO:0000269|PubMed:28189088}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with host PPP1CB.
CC       {ECO:0000269|PubMed:17215279}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:32075923}.
CC   -!- SIMILARITY: Belongs to the asfivirus DP71L family. {ECO:0000305}.
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DR   EMBL; U18466; AAA65380.1; -; Genomic_DNA.
DR   RefSeq; NP_042844.1; NC_001659.2.
DR   SMR; Q65212; -.
DR   GeneID; 22220380; -.
DR   KEGG; vg:22220380; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0039586; P:modulation by virus of host PP1 activity; IEA:UniProtKB-KW.
DR   GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR019523; Prot_Pase1_reg-su15A/B_C.
DR   Pfam; PF10488; PP1c_bdg; 1.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Inhibition of host innate immune response by virus;
KW   Inhibition of host interferon signaling pathway by virus; Late protein;
KW   Modulation of host PP1 activity by virus; Reference proteome;
KW   Viral immunoevasion.
FT   CHAIN           1..71
FT                   /note="Protein DP71L"
FT                   /id="PRO_0000379083"
FT   REGION          16..18
FT                   /note="Important for host CHOP inhibition"
FT                   /evidence="ECO:0000269|PubMed:28189088"
FT   REGION          57..61
FT                   /note="Important for host CHOP inhibition"
FT                   /evidence="ECO:0000269|PubMed:28189088"
FT   MUTAGEN         16
FT                   /note="V->E: 83% loss of host CHOP inhibition; when
FT                   associated with L-18."
FT                   /evidence="ECO:0000269|PubMed:28189088"
FT   MUTAGEN         18
FT                   /note="F->L: 83% loss of host CHOP inhibition;when
FT                   associated with E-16."
FT                   /evidence="ECO:0000269|PubMed:28189088"
FT   MUTAGEN         57
FT                   /note="L->A: 69% loss of host CHOP inhibition."
FT                   /evidence="ECO:0000269|PubMed:28189088"
FT   MUTAGEN         61
FT                   /note="L->A: 43% loss of host CHOP inhibition."
FT                   /evidence="ECO:0000269|PubMed:28189088"
SQ   SEQUENCE   71 AA;  8478 MW;  A1F341352EE2B6DE CRC64;
     MGGRRRKKRT NDTKHVRFAA AVEVWEADDI ERKGPWEQVA VDRFRFQRRI ASVEELLSTV
     LLRQKKLLEQ Q