DPA5A_MOUSE
ID DPA5A_MOUSE Reviewed; 118 AA.
AC Q9CQS7;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Developmental pluripotency-associated protein 5A;
DE Short=mDppa5;
DE AltName: Full=ES cell-associated transcript 2 protein;
DE AltName: Full=Embryonal stem cell-specific gene 1 protein;
DE Short=ESG-1;
DE AltName: Full=Protein pH 34;
GN Name=Dppa5a; Synonyms=Dppa5, Ecat2, Esg1, Ph34;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, AND TISSUE SPECIFICITY.
RX PubMed=2044862; DOI=10.1111/j.1432-0436.1991.tb00866.x;
RA Astigiano S., Barkai U., Abarzua P., Tan S.C., Harper M.I., Sherman M.I.;
RT "Changes in gene expression following exposure of nulli-SCCl murine
RT embryonal carcinoma cells to inducers of differentiation: characterization
RT of a down-regulated mRNA.";
RL Differentiation 46:61-67(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=12620990; DOI=10.1242/dev.00366;
RA Bortvin A., Eggan K., Skaletsky H., Akutsu H., Berry D.L., Yanagimachi R.,
RA Page D.C., Jaenisch R.;
RT "Incomplete reactivation of Oct4-related genes in mouse embryos cloned from
RT somatic nuclei.";
RL Development 130:1673-1680(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Blastocyst, and Embryonic stem cell;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic germ cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=8123591;
RA Bierbaum P., MacLean-Hunter S., Ehlert F., Moeroey T., Mueller R.;
RT "Cloning of embryonal stem cell-specific genes: characterization of the
RT transcriptionally controlled gene esg-1.";
RL Cell Growth Differ. 5:37-46(1994).
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=12466296; DOI=10.1101/gr.670002;
RA Tanaka T.S., Kunath T., Kimber W.L., Jaradat S.A., Stagg C.A., Usuda M.,
RA Yokota T., Niwa H., Rossant J., Ko M.S.;
RT "Gene expression profiling of embryo-derived stem cells reveals candidate
RT genes associated with pluripotency and lineage specificity.";
RL Genome Res. 12:1921-1928(2002).
RN [7]
RP INDUCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=15790765; DOI=10.1634/stemcells.2004-0245;
RA Kim S.-K., Suh M.R., Yoon H.S., Lee J.B., Oh S.K., Moon S.-Y., Moon S.H.,
RA Lee J.Y., Hwang J.H., Cho W.J., Kim K.-S.;
RT "Identification of developmental pluripotency associated 5 expression in
RT human pluripotent stem cells.";
RL Stem Cells 23:458-462(2005).
RN [8]
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=16166252; DOI=10.1634/stemcells.2005-0146;
RA Western P., Maldonado-Saldivia J., van den Bergen J., Hajkova P.,
RA Saitou M., Barton S., Surani M.A.;
RT "Analysis of Esg1 expression in pluripotent cells and the germline reveals
RT similarities with Oct4 and Sox2 and differences between human pluripotent
RT cell lines.";
RL Stem Cells 23:1436-1442(2005).
RN [9]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=16504174; DOI=10.1186/1471-213x-6-11;
RA Amano H., Itakura K., Maruyama M., Ichisaka T., Nakagawa M., Yamanaka S.;
RT "Identification and targeted disruption of the mouse gene encoding ESG1
RT (PH34/ECAT2/DPPA5).";
RL BMC Dev. Biol. 6:11-11(2006).
RN [10]
RP SUBCELLULAR LOCATION, RNA-BINDING, AND DEVELOPMENTAL STAGE.
RX PubMed=16872451; DOI=10.1111/j.1440-169x.2006.00875.x;
RA Tanaka T.S., Lopez de Silanes I., Sharova L.V., Akutsu H., Yoshikawa T.,
RA Amano H., Yamanaka S., Gorospe M., Ko M.S.;
RT "Esg1, expressed exclusively in preimplantation embryos, germline, and
RT embryonic stem cells, is a putative RNA-binding protein with broad RNA
RT targets.";
RL Dev. Growth Differ. 48:381-390(2006).
RN [11]
RP IDENTIFICATION.
RX PubMed=17913455; DOI=10.1016/j.ygeno.2007.06.003;
RA Pierre A., Gautier M., Callebaut I., Bontoux M., Jeanpierre E.,
RA Pontarotti P., Monget P.;
RT "Atypical structure and phylogenomic evolution of the new eutherian
RT oocyte- and embryo-expressed KHDC1/DPPA5/ECAT1/OOEP gene family.";
RL Genomics 90:583-594(2007).
CC -!- FUNCTION: Involved in the maintenance of embryonic stem (ES) cell
CC pluripotency. Dispensable for self-renewal of pluripotent ES cells and
CC establishment of germ cells. Associates with specific target mRNAs.
CC {ECO:0000269|PubMed:16504174}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16166252,
CC ECO:0000269|PubMed:16872451}.
CC -!- DEVELOPMENTAL STAGE: Pluripotent cell-specific. Expressed in zygotes,
CC cleavage-stage embryos and blastocysts, embryonic stem (ES) and
CC embryonic germ (EG) cells. Detected in both the trophectoderm (TE) and
CC inner cell mass (ICM) of blastocysts. More abundant in the ICM than TE
CC at 3.5 dpc blastocyst stage. Expressed in primordial germ (PGC) cells
CC from 10.5 to 13.5 dpc. Expressed in developing gonads from 11.5 to 15.5
CC dpc. Progressively undetectable in ovary from 13.5 to 15.5 dpc.
CC Undetectable in testis after 15.5 dpc. Not expressed in somatic cells
CC at 13.5 dpc (at protein level). Expressed in embryonic stem (ES) and
CC embryonic carcinoma (EC) cells. Not detected during the differentiation
CC of stem cells or midgestation embryos nor in neonatal tissues. Weakly
CC expressed or not detected in oocytes and fertilized eggs.
CC {ECO:0000269|PubMed:12466296, ECO:0000269|PubMed:12620990,
CC ECO:0000269|PubMed:15790765, ECO:0000269|PubMed:16166252,
CC ECO:0000269|PubMed:16504174, ECO:0000269|PubMed:16872451,
CC ECO:0000269|PubMed:8123591}.
CC -!- INDUCTION: Down-regulated by retinoic acid in embryonic carcinoma (EC)
CC cells and in developing germ cells. {ECO:0000269|PubMed:15790765,
CC ECO:0000269|PubMed:2044862}.
CC -!- DISRUPTION PHENOTYPE: Mice develop normally and are fertile. ES cells
CC derived from these mice demonstrate normal morphology, proliferation
CC and differentiation. {ECO:0000269|PubMed:16504174}.
CC -!- SIMILARITY: Belongs to the KHDC1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF490349; AAO84507.1; -; mRNA.
DR EMBL; AK010519; BAB27001.2; -; mRNA.
DR EMBL; AK010584; BAB27045.2; -; mRNA.
DR EMBL; AK145761; BAE26633.1; -; mRNA.
DR EMBL; BC092353; AAH92353.1; -; mRNA.
DR EMBL; BC092354; AAH92354.1; -; mRNA.
DR EMBL; BC095979; AAH95979.1; -; mRNA.
DR CCDS; CCDS23360.1; -.
DR PIR; A55058; A55058.
DR RefSeq; NP_079550.2; NM_025274.3.
DR AlphaFoldDB; Q9CQS7; -.
DR SMR; Q9CQS7; -.
DR STRING; 10090.ENSMUSP00000071881; -.
DR iPTMnet; Q9CQS7; -.
DR PhosphoSitePlus; Q9CQS7; -.
DR REPRODUCTION-2DPAGE; Q9CQS7; -.
DR PaxDb; Q9CQS7; -.
DR PeptideAtlas; Q9CQS7; -.
DR PRIDE; Q9CQS7; -.
DR ProteomicsDB; 279801; -.
DR Antibodypedia; 46318; 164 antibodies from 22 providers.
DR DNASU; 434423; -.
DR Ensembl; ENSMUST00000071991; ENSMUSP00000071881; ENSMUSG00000060461.
DR GeneID; 434423; -.
DR KEGG; mmu:434423; -.
DR UCSC; uc009qug.2; mouse.
DR CTD; 434423; -.
DR MGI; MGI:101800; Dppa5a.
DR VEuPathDB; HostDB:ENSMUSG00000060461; -.
DR eggNOG; ENOG502RW7D; Eukaryota.
DR GeneTree; ENSGT00940000154353; -.
DR HOGENOM; CLU_169085_0_0_1; -.
DR InParanoid; Q9CQS7; -.
DR OMA; GGSRIPY; -.
DR OrthoDB; 1502478at2759; -.
DR PhylomeDB; Q9CQS7; -.
DR TreeFam; TF338690; -.
DR BioGRID-ORCS; 434423; 12 hits in 54 CRISPR screens.
DR ChiTaRS; Dppa5a; mouse.
DR PRO; PR:Q9CQS7; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9CQS7; protein.
DR Bgee; ENSMUSG00000060461; Expressed in morula and 49 other tissues.
DR Genevisible; Q9CQS7; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0003729; F:mRNA binding; IDA:MGI.
DR GO; GO:0010468; P:regulation of gene expression; IMP:MGI.
DR CDD; cd12795; FILIA_N_like; 1.
DR Gene3D; 3.30.1370.10; -; 1.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR031952; MOEP19_KH-like.
DR Pfam; PF16005; MOEP19; 1.
DR SUPFAM; SSF54791; SSF54791; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Reference proteome; RNA-binding.
FT CHAIN 1..118
FT /note="Developmental pluripotency-associated protein 5A"
FT /id="PRO_0000311976"
FT DOMAIN 24..86
FT /note="KH; atypical"
SQ SEQUENCE 118 AA; 13810 MW; 30233CB5C536AC55 CRC64;
MMVTLVTRKD IPPWVKVPED LKDPEVFQVQ SLVLKYLFGP QGSRMSHIEQ VSQAMFELKN
LESPEELIEV FIYGSQNNKI RAKWMLQSMA ERYHLRQQKG VLKLEESMKT LELGQCIE
