DPB31_ARATH
ID DPB31_ARATH Reviewed; 206 AA.
AC Q9LN09; Q0WP19;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=DNA polymerase II subunit B3-1 {ECO:0000303|PubMed:25490919};
DE AltName: Full=Nuclear factor Y, subunit C10 {ECO:0000303|PubMed:25490919};
DE Short=AtNF-YC10 {ECO:0000303|PubMed:25490919};
GN Name=DPB3-1 {ECO:0000303|PubMed:25490919};
GN Synonyms=NF-YC10 {ECO:0000303|PubMed:25490919};
GN OrderedLocusNames=At1g07980 {ECO:0000312|Araport:AT1G07980};
GN ORFNames=T6D22.7 {ECO:0000312|EMBL:AAF79844.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH DREB2A; NFYB1; NFYB2;
RP NFYB3; NFYB6; NFYB7 AND DPB4, SUBUNIT, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION BY HEAT AND DEHYDRATION.
RC STRAIN=cv. Columbia;
RX PubMed=25490919; DOI=10.1105/tpc.114.132928;
RA Sato H., Mizoi J., Tanaka H., Maruyama K., Qin F., Osakabe Y., Morimoto K.,
RA Ohori T., Kusakabe K., Nagata M., Shinozaki K., Yamaguchi-Shinozaki K.;
RT "Arabidopsis DPB3-1, a DREB2A interactor, specifically enhances heat
RT stress-induced gene expression by forming a heat stress-specific
RT transcriptional complex with NF-Y subunits.";
RL Plant Cell 26:4954-4973(2014).
RN [7]
RP REVIEW.
RX PubMed=28119722; DOI=10.3389/fpls.2016.02045;
RA Zhao H., Wu D., Kong F., Lin K., Zhang H., Li G.;
RT "The Arabidopsis thaliana Nuclear Factor Y Transcription Factors.";
RL Front. Plant Sci. 7:2045-2045(2016).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH GAPC.
RC STRAIN=cv. Columbia;
RX PubMed=32651385; DOI=10.1038/s41467-020-17311-4;
RA Kim S.-C., Guo L., Wang X.;
RT "Nuclear moonlighting of cytosolic glyceraldehyde-3-phosphate dehydrogenase
RT regulates Arabidopsis response to heat stress.";
RL Nat. Commun. 11:3439-3439(2020).
CC -!- FUNCTION: Transcription factor which promotes the expression of heat
CC stress-inducible genes by contributing to the formation of a heat
CC stress-specific transcriptional complex with NF-Y subunits (e.g. NF-YA2
CC and NF-YB3) and DREB2A at the promoter of target genes, thus promoting
CC heat tolerance (PubMed:25490919). Together with the glyceraldehyde-3-
CC phosphate dehydrogenase (GAPC), enhances heat tolerance
CC (PubMed:32651385). {ECO:0000269|PubMed:25490919,
CC ECO:0000269|PubMed:32651385}.
CC -!- SUBUNIT: Interacts with DREB2A in the nucleus during heat-stress
CC (PubMed:25490919). Component of a heat stress-inducible transcriptional
CC complex with NF-YA and NF-YB subunits made, at least, of NFYA2, NFYB3
CC and DPB3-1 in cooperation with DREB2A (PubMed:25490919). Binds directly
CC with NFYB1, NFYB2, NFYB3, NFYB6, NFYB7, and DPB4 (PubMed:25490919).
CC Binds to GAPC in response to heat-stress; this interaction promotes
CC DNA-binding ability to its target promoter (PubMed:32651385).
CC {ECO:0000269|PubMed:25490919, ECO:0000269|PubMed:32651385}.
CC -!- INTERACTION:
CC Q9LN09; Q9ZQH2: NF-YB11; NbExp=3; IntAct=EBI-4461992, EBI-15202674;
CC Q9LN09; Q9FGJ3: NFYB2; NbExp=4; IntAct=EBI-4461992, EBI-15192505;
CC Q9LN09; O23310: NFYB3; NbExp=4; IntAct=EBI-4461992, EBI-4452064;
CC Q9LN09; Q84W66-2: NFYB6; NbExp=3; IntAct=EBI-4461992, EBI-15191739;
CC Q9LN09; Q9SIT9: NFYB7; NbExp=3; IntAct=EBI-4461992, EBI-4459822;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000269|PubMed:25490919}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, petioles, cotyledons,
CC hypocotyls and root tips. {ECO:0000269|PubMed:25490919}.
CC -!- DEVELOPMENTAL STAGE: In seedlings, first expressed in petioles and leaf
CC blades of the cotyledons as well as tops and bottoms of the hypocotyls
CC (PubMed:25490919). Later present in whole hypocotyls and weakly in the
CC cotyledons and root tips (PubMed:25490919).
CC {ECO:0000269|PubMed:25490919}.
CC -!- INDUCTION: Enhanced by heat stress but repressed by dehydration stress
CC in young seedlings but not in older plants.
CC {ECO:0000269|PubMed:25490919}.
CC -!- DISRUPTION PHENOTYPE: Reduced expression of heat stress-inducible genes
CC leading to an enhanced sensitivity to heat stress (PubMed:25490919).
CC Impaired ability of GAPC to enhance heat tolerance of seedlings and to
CC promote the expression of heat-inducible genes (PubMed:32651385).
CC {ECO:0000269|PubMed:25490919, ECO:0000269|PubMed:32651385}.
CC -!- SIMILARITY: Belongs to the NFYC/HAP5 subunit family. {ECO:0000305}.
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DR EMBL; AC026875; AAF79844.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28222.1; -; Genomic_DNA.
DR EMBL; BT025266; ABF19019.1; -; mRNA.
DR EMBL; AK229011; BAF00898.1; -; mRNA.
DR EMBL; AK229266; BAF01130.1; -; mRNA.
DR EMBL; AY086815; AAM63864.1; -; mRNA.
DR RefSeq; NP_563803.1; NM_100672.3.
DR AlphaFoldDB; Q9LN09; -.
DR SMR; Q9LN09; -.
DR IntAct; Q9LN09; 11.
DR STRING; 3702.AT1G07980.1; -.
DR PaxDb; Q9LN09; -.
DR PRIDE; Q9LN09; -.
DR ProteomicsDB; 189344; -.
DR EnsemblPlants; AT1G07980.1; AT1G07980.1; AT1G07980.
DR GeneID; 837313; -.
DR Gramene; AT1G07980.1; AT1G07980.1; AT1G07980.
DR Araport; AT1G07980; -.
DR TAIR; locus:2205170; AT1G07980.
DR eggNOG; KOG1657; Eukaryota.
DR HOGENOM; CLU_100368_0_1_1; -.
DR InParanoid; Q9LN09; -.
DR OMA; EWERGMT; -.
DR OrthoDB; 1498402at2759; -.
DR PhylomeDB; Q9LN09; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LN09; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009408; P:response to heat; IDA:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IEP:UniProtKB.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR003958; CBFA_NFYB_domain.
DR InterPro; IPR009072; Histone-fold.
DR Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..206
FT /note="DNA polymerase II subunit B3-1"
FT /id="PRO_0000455274"
FT REGION 1..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 33..40
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 1..17
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..75
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..108
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 2
FT /note="V -> M (in Ref. 4; BAF00898/BAF01130)"
FT /evidence="ECO:0000305"
FT CONFLICT 57
FT /note="S -> R (in Ref. 4; BAF00898/BAF01130)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 206 AA; 23027 MW; 33886B934FFF89BF CRC64;
MVSSKKPKEK KARSDVVVNK ASGRSKRSSG SRTKKTSNKV NIVKKKPEIY EISESSSSDS
VEEAIRGDEA KKSNGVVSKR GNGKSVGIPT KTSKNREEDD GGAEDAKIKF PMNRIRRIMR
SDNSAPQIMQ DAVFLVNKAT EMFIERFSEE AYDSSVKDKK KFIHYKHLSS VVSNDQRYEF
LADSVPEKLK AEAALEEWER GMTDAG
