ADEC2_AGRFC
ID ADEC2_AGRFC Reviewed; 597 AA.
AC Q7CUX4; Q8U7M3;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Adenine deaminase 2 {ECO:0000255|HAMAP-Rule:MF_01518};
DE Short=Adenase 2 {ECO:0000255|HAMAP-Rule:MF_01518};
DE Short=Adenine aminase 2 {ECO:0000255|HAMAP-Rule:MF_01518};
DE EC=3.5.4.2 {ECO:0000255|HAMAP-Rule:MF_01518};
GN Name=ade2 {ECO:0000255|HAMAP-Rule:MF_01518}; Synonyms=adeC;
GN OrderedLocusNames=Atu4426; ORFNames=AGR_L_883;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenine + H(+) + H2O = hypoxanthine + NH4(+);
CC Xref=Rhea:RHEA:23688, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16708, ChEBI:CHEBI:17368, ChEBI:CHEBI:28938; EC=3.5.4.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01518};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01518};
CC -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC Adenine deaminase family. {ECO:0000255|HAMAP-Rule:MF_01518}.
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DR EMBL; AE007870; AAK89014.1; -; Genomic_DNA.
DR PIR; AF3100; AF3100.
DR PIR; D98186; D98186.
DR RefSeq; NP_356229.1; NC_003063.2.
DR RefSeq; WP_010973838.1; NC_003063.2.
DR PDB; 3NQB; X-ray; 2.21 A; A/B=1-597.
DR PDB; 3T81; X-ray; 2.63 A; A/B=1-597.
DR PDB; 3T8L; X-ray; 2.80 A; A/B=1-597.
DR PDBsum; 3NQB; -.
DR PDBsum; 3T81; -.
DR PDBsum; 3T8L; -.
DR AlphaFoldDB; Q7CUX4; -.
DR SMR; Q7CUX4; -.
DR STRING; 176299.Atu4426; -.
DR DNASU; 1136300; -.
DR EnsemblBacteria; AAK89014; AAK89014; Atu4426.
DR GeneID; 66224619; -.
DR KEGG; atu:Atu4426; -.
DR PATRIC; fig|176299.10.peg.4234; -.
DR eggNOG; COG1001; Bacteria.
DR HOGENOM; CLU_027935_0_0_5; -.
DR OMA; IEGHFPG; -.
DR PhylomeDB; Q7CUX4; -.
DR BioCyc; AGRO:ATU4426-MON; -.
DR EvolutionaryTrace; Q7CUX4; -.
DR Proteomes; UP000000813; Chromosome linear.
DR GO; GO:0000034; F:adenine deaminase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006146; P:adenine catabolic process; IEA:InterPro.
DR CDD; cd01295; AdeC; 1.
DR Gene3D; 2.30.40.10; -; 1.
DR HAMAP; MF_01518; Adenine_deamin; 1.
DR InterPro; IPR006679; Adenine_deam.
DR InterPro; IPR026912; Adenine_deam_C.
DR InterPro; IPR006680; Amidohydro-rel.
DR InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR InterPro; IPR032466; Metal_Hydrolase.
DR PANTHER; PTHR11113:SF2; PTHR11113:SF2; 1.
DR Pfam; PF13382; Adenine_deam_C; 1.
DR Pfam; PF01979; Amidohydro_1; 1.
DR SUPFAM; SSF51338; SSF51338; 1.
DR SUPFAM; SSF51556; SSF51556; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Manganese; Reference proteome.
FT CHAIN 1..597
FT /note="Adenine deaminase 2"
FT /id="PRO_0000142399"
FT HELIX 11..13
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 16..27
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 32..38
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 40..42
FT /evidence="ECO:0007829|PDB:3NQB"
FT TURN 44..46
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 53..57
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 60..65
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:3T8L"
FT STRAND 73..78
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 82..86
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 88..93
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 95..98
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 102..110
FT /evidence="ECO:0007829|PDB:3NQB"
FT TURN 111..113
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 114..119
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 122..128
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 130..140
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 144..151
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 155..158
FT /evidence="ECO:0007829|PDB:3NQB"
FT TURN 159..161
FT /evidence="ECO:0007829|PDB:3T8L"
FT HELIX 170..177
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 182..188
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 191..195
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 199..211
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 214..217
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 224..232
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 244..252
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 256..263
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 264..266
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 267..277
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 284..287
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 293..298
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 302..311
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 316..323
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 325..331
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 336..338
FT /evidence="ECO:0007829|PDB:3T81"
FT STRAND 347..351
FT /evidence="ECO:0007829|PDB:3NQB"
FT TURN 353..355
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 358..363
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 366..370
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 373..376
FT /evidence="ECO:0007829|PDB:3T81"
FT HELIX 384..386
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 397..400
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 405..419
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 421..430
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 431..434
FT /evidence="ECO:0007829|PDB:3T81"
FT STRAND 440..446
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 449..452
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 456..464
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 468..474
FT /evidence="ECO:0007829|PDB:3NQB"
FT TURN 477..479
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 482..488
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 489..501
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 504..510
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 513..519
FT /evidence="ECO:0007829|PDB:3NQB"
FT TURN 523..525
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 526..529
FT /evidence="ECO:0007829|PDB:3T81"
FT HELIX 531..548
FT /evidence="ECO:0007829|PDB:3NQB"
FT TURN 553..555
FT /evidence="ECO:0007829|PDB:3NQB"
FT HELIX 558..562
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 569..571
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 576..580
FT /evidence="ECO:0007829|PDB:3NQB"
FT TURN 582..584
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 587..589
FT /evidence="ECO:0007829|PDB:3NQB"
FT STRAND 591..594
FT /evidence="ECO:0007829|PDB:3NQB"
SQ SEQUENCE 597 AA; 62811 MW; 21D10CF0B7A7478B CRC64;
MTAQIRLAEP ADLNDDTLRA RAVAAARGDQ RFDVLITGGT LVDVVTGELR PADIGIVGAL
IASVHEPASR RDAAQVIDAG GAYVSPGLID THMHIESSMI TPAAYAAAVV ARGVTTIVWD
PHEFGNVHGV DGVRWAAKAI ENLPLRAILL APSCVPSAPG LERGGADFDA AILADLLSWP
EIGGIAEIMN MRGVIERDPR MSGIVQAGLA AEKLVCGHAR GLKNADLNAF MAAGVSSDHE
LVSGEDLMAK LRAGLTIELR GSHDHLLPEF VAALNTLGHL PQTVTLCTDD VFPDDLLQGG
GLDDVVRRLV RYGLKPEWAL RAATLNAAQR LGRSDLGLIA AGRRADIVVF EDLNGFSARH
VLASGRAVAE GGRMLVDIPT CDTTVLKGSM KLPLRMANDF LVKSQGAKVR LATIDRPRFT
QWGETEADVK DGFVVPPEGA TMISVTHRHG MAEPTTKTGF LTGWGRWNGA FATTVSHDSH
NLTVFGGNAG DMALAANAVI GTGGGMAVAS EGKVTAILPL PLSGLVSDAP LEEVARAFED
LREAVGKVVE WQPPYLVFKA CFGATLACNI GPHQTDMGIA DVLTGKVMES PVIEVLG
