ID   ADEC2_BRASO             Reviewed;         600 AA.
AC   A4YXM5;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Adenine deaminase 2 {ECO:0000255|HAMAP-Rule:MF_01518};
DE            Short=Adenase 2 {ECO:0000255|HAMAP-Rule:MF_01518};
DE            Short=Adenine aminase 2 {ECO:0000255|HAMAP-Rule:MF_01518};
DE            EC=3.5.4.2 {ECO:0000255|HAMAP-Rule:MF_01518};
GN   Name=ade2 {ECO:0000255|HAMAP-Rule:MF_01518}; OrderedLocusNames=BRADO4947;
OS   Bradyrhizobium sp. (strain ORS 278).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium.
OX   NCBI_TaxID=114615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ORS 278;
RX   PubMed=17540897; DOI=10.1126/science.1139548;
RA   Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C.,
RA   Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA   Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA   Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E.,
RA   Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA   Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT   "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL   Science 316:1307-1312(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenine + H(+) + H2O = hypoxanthine + NH4(+);
CC         Xref=Rhea:RHEA:23688, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16708, ChEBI:CHEBI:17368, ChEBI:CHEBI:28938; EC=3.5.4.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01518};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01518};
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC       Adenine deaminase family. {ECO:0000255|HAMAP-Rule:MF_01518}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CU234118; CAL78651.1; -; Genomic_DNA.
DR   RefSeq; WP_011927749.1; NC_009445.1.
DR   AlphaFoldDB; A4YXM5; -.
DR   SMR; A4YXM5; -.
DR   STRING; 114615.BRADO4947; -.
DR   EnsemblBacteria; CAL78651; CAL78651; BRADO4947.
DR   KEGG; bra:BRADO4947; -.
DR   eggNOG; COG1001; Bacteria.
DR   HOGENOM; CLU_027935_0_0_5; -.
DR   OMA; MVTACAY; -.
DR   OrthoDB; 751534at2; -.
DR   BioCyc; BSP114615:BRADO_RS22995-MON; -.
DR   Proteomes; UP000001994; Chromosome.
DR   GO; GO:0000034; F:adenine deaminase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006146; P:adenine catabolic process; IEA:InterPro.
DR   CDD; cd01295; AdeC; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   HAMAP; MF_01518; Adenine_deamin; 1.
DR   InterPro; IPR006679; Adenine_deam.
DR   InterPro; IPR026912; Adenine_deam_C.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   PANTHER; PTHR11113:SF2; PTHR11113:SF2; 1.
DR   Pfam; PF13382; Adenine_deam_C; 1.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   SUPFAM; SSF51338; SSF51338; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Manganese; Reference proteome.
FT   CHAIN           1..600
FT                   /note="Adenine deaminase 2"
FT                   /id="PRO_0000296718"
SQ   SEQUENCE   600 AA;  63684 MW;  F834C262DE9D8F11 CRC64;
     MTNLTRFSVA PLHTMTRRLA DVASGRVAPD LVITGARVLS TYSERILRDR ELWITGGRVA
     AVKPAGAHKA LPSGFTIYDA AGGIIAPGLV DPHIHIESSM VTACAYAEAA LLNGTTTIFC
     DSHEIGNVMD TAGVEAMLED ARQAPLSIFL TVPSTVPATS AALETAGGDL TPDKIAGLFD
     RWPEAVALGE KMDFVPVCMG DERSHAILAA ALQRGRPVSG HVYGREFVAA YAASGVTDTH
     EAIDRDIADD LLDAGVWIFL RGGPPTTPWH SLPQAIRTIT ELGASHKRTA VCTDDRDADD
     LMLFGLDWVV REAVKAGMSP EQAWSMGSLH GATRFAMDGE IGGLGGGRRA DLVLLDDGLK
     PQSTWYGGEL VVDQGKITSL LDQALSQRYQ YPKAAYATVK LPAQMKLTPE LPAKACTVNA
     IKTALPGITL IHEKVAIKPA NDWDTLFARH GLCFVAVIER HGKSAGNVAH GLLKDFGLKR
     GAVASSVGHD SHNIIVAGTN EADMQTAVAA IGAQQGGVCV VADGKVRAMV PLPIAGLLSD
     KRVTAVAEEV KLLKKEWAEA GCTIPYMGFN LIPLSVIPEI RITDKGLVLV PQMELAPLFE