ID   DPDA_SALMO              Reviewed;         416 AA.
AC   P0DW33;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=DNA-guanine transglycosylase {ECO:0000305};
DE            EC=2.4.2.- {ECO:0000305|PubMed:30159947};
GN   Name=dpdA {ECO:0000303|PubMed:26929322};
GN   Synonyms=tgtA5 {ECO:0000303|PubMed:26929322};
GN   ORFNames=SEEM315_10824 {ECO:0000312|EMBL:EFY12575.1};
OS   Salmonella montevideo.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=115981;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=315996572;
RX   PubMed=21345093; DOI=10.1056/nejmc1100443;
RA   Lienau E.K., Strain E., Wang C., Zheng J., Ottesen A.R., Keys C.E.,
RA   Hammack T.S., Musser S.M., Brown E.W., Allard M.W., Cao G., Meng J.,
RA   Stones R.;
RT   "Identification of a salmonellosis outbreak by means of molecular
RT   sequencing.";
RL   N. Engl. J. Med. 364:981-982(2011).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC BAA-710;
RX   PubMed=26929322; DOI=10.1073/pnas.1518570113;
RA   Thiaville J.J., Kellner S.M., Yuan Y., Hutinet G., Thiaville P.C.,
RA   Jumpathong W., Mohapatra S., Brochier-Armanet C., Letarov A.V.,
RA   Hillebrand R., Malik C.K., Rizzo C.J., Dedon P.C., de Crecy-Lagard V.;
RT   "Novel genomic island modifies DNA with 7-deazaguanine derivatives.";
RL   Proc. Natl. Acad. Sci. U.S.A. 113:E1452-E1459(2016).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC BAA-710;
RX   PubMed=30159947; DOI=10.1111/mmi.14113;
RA   Yuan Y., Hutinet G., Valera J.G., Hu J., Hillebrand R., Gustafson A.,
RA   Iwata-Reuyl D., Dedon P.C., de Crecy-Lagard V.;
RT   "Identification of the minimal bacterial 2'-deoxy-7-amido-7-deazaguanine
RT   synthesis machinery.";
RL   Mol. Microbiol. 110:469-483(2018).
CC   -!- FUNCTION: Part of the dpd cluster involved in the insertion of 7-
CC       deazaguanine derivatives in DNA (PubMed:26929322, PubMed:30159947).
CC       DpdA may insert 7-cyano-7-deazaguanine (preQ0) into DNA with the help
CC       of DpdB (PubMed:30159947). DpdA and dpdB are necessary and sufficient
CC       to synthesize 2'-deoxy-7-cyano-7-deazaguanosine (dPreQ0)
CC       (PubMed:30159947). {ECO:0000269|PubMed:26929322,
CC       ECO:0000269|PubMed:30159947}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000250|UniProtKB:P28720};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:P28720};
CC   -!- DISRUPTION PHENOTYPE: Mutant lacking the whole dpd gene cluster does
CC       not contain dPreQ0 and 2'-deoxy-7-amido-7-deazaguanosine (dADG) in DNA
CC       (PubMed:26929322). DpdA deletion mutant (in a dpdD mutant background)
CC       lacks both dPreQ0 and dADG (PubMed:30159947).
CC       {ECO:0000269|PubMed:26929322, ECO:0000269|PubMed:30159947}.
CC   -!- SIMILARITY: Belongs to the DNA-guanine transglycosylase family.
CC       {ECO:0000305}.
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DR   EMBL; AESH01000017; EFY12575.1; -; Genomic_DNA.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Metal-binding; Transferase; Zinc.
FT   CHAIN           1..416
FT                   /note="DNA-guanine transglycosylase"
FT                   /id="PRO_0000456249"
FT   ACT_SITE        95
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P28720"
FT   ACT_SITE        256
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:P28720"
FT   BINDING         368
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P28720"
FT   BINDING         370
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P28720"
FT   BINDING         373
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P28720"
FT   BINDING         395
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P28720"
SQ   SEQUENCE   416 AA;  48101 MW;  E8073C9405E2C801 CRC64;
     MSKLKYFFPD SQDFIDPSFD FVRETRNEHR VRQRDDHYPH EVFPHPYDGM LVSKAVVDGL
     GGGESKYTRA QRLRYFRNGM KHFFRLPDNM QTMGDCGAFT YVNQDVPPYR VEEVIEFYET
     SRFNYGVSLD HIIFGYEKPG ESFSGEVLAE CRRRQDITLT LAQDFLVKSQ KSCFTPFGVA
     HGWNKKSYRQ SVEALLAMGY KNITMGGMVP LKTAQILETL EEIKPLLKSD TQVHLLGIAR
     PESFADFIRL GVTSIDSTTP LQQAFKDRKN NYHTPEGRAY TAVRVPQFDA NPSLSRKIKS
     GVIDQDVARH LEKDAMHALF EYDNNALSLE KTLEAVLAYE RLHSGEKEAE KIRADYERTL
     GDRPWRKCEC NICRSIGINV IIFRGAERNR RRGFHNIQVL YNRLQYTLSL RSEDKS