DPE2_ORYSJ
ID DPE2_ORYSJ Reviewed; 946 AA.
AC Q69Q02; A0A0P0XA64;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=4-alpha-glucanotransferase DPE2;
DE EC=2.4.1.25;
DE AltName: Full=Amylomaltase;
DE AltName: Full=Disproportionating enzyme;
DE Short=D-enzyme;
DE AltName: Full=Protein DISPROPORTIONATING ENZYME 2;
GN Name=DPE2; OrderedLocusNames=Os07g0662900, LOC_Os07g46790;
GN ORFNames=P0453E03.120;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Cytosolic alpha-glucanotransferase essential for the
CC cytosolic metabolism of maltose, an intermediate on the pathway by
CC which starch is converted to sucrose in leaves at night. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the disproportionating enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK067082; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AP005452; BAD31425.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF22461.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT03073.1; -; Genomic_DNA.
DR EMBL; AK067082; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015647212.1; XM_015791726.1.
DR AlphaFoldDB; Q69Q02; -.
DR STRING; 4530.OS07T0662900-01; -.
DR PaxDb; Q69Q02; -.
DR PRIDE; Q69Q02; -.
DR EnsemblPlants; Os07t0662900-01; Os07t0662900-01; Os07g0662900.
DR GeneID; 4344192; -.
DR Gramene; Os07t0662900-01; Os07t0662900-01; Os07g0662900.
DR KEGG; osa:4344192; -.
DR eggNOG; ENOG502QR3V; Eukaryota.
DR HOGENOM; CLU_014132_0_0_1; -.
DR InParanoid; Q69Q02; -.
DR OMA; WTDSYPY; -.
DR OrthoDB; 302364at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR ExpressionAtlas; Q69Q02; baseline and differential.
DR Genevisible; Q69Q02; OS.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0010297; F:heteropolysaccharide binding; IEA:EnsemblPlants.
DR GO; GO:2001070; F:starch binding; IEA:InterPro.
DR GO; GO:0000025; P:maltose catabolic process; IEA:EnsemblPlants.
DR CDD; cd05815; CBM20_DPE2_repeat1; 1.
DR CDD; cd05816; CBM20_DPE2_repeat2; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR034840; CBM20_DPE2_1.
DR InterPro; IPR034841; CBM20_DPE2_2.
DR InterPro; IPR002044; CBM_fam20.
DR InterPro; IPR003385; Glyco_hydro_77.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF00686; CBM_20; 2.
DR Pfam; PF02446; Glyco_hydro_77; 1.
DR SMART; SM01065; CBM_2; 2.
DR SUPFAM; SSF49452; SSF49452; 2.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS51166; CBM20; 2.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cytoplasm; Glycosyltransferase;
KW Reference proteome; Repeat; Transferase.
FT CHAIN 1..946
FT /note="4-alpha-glucanotransferase DPE2"
FT /id="PRO_0000407921"
FT DOMAIN 7..115
FT /note="CBM20 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00594"
FT DOMAIN 150..264
FT /note="CBM20 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00594"
FT REGION 919..946
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 275
FT /note="R -> G (in Ref. 4; AK067082)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 946 AA; 108294 MW; EA8ED92EF3EB15CF CRC64;
MTNLSGKKSL NTVTLVFKLP YYTQWGQSLL IAGSEPALGS WNVKQGLSLS PVHQGNELIW
SGRVSVATGF TCQYNYYVVD DNKNVLRSES GEKRKLVLPE GVQDGDVVEI RDWWQDASEA
LFLRSAFKNV IFNGSENAKR ELKTTSLNKS LEPEDIVVQF IVSCPRLGAG STVVVTGSNP
QLGRWQTQDG LKLNYVGDSI WKANCLLRKS EFPIKYKYCK ISEAGVSSLE FGPNREADVD
LSSPKPSRYV LLSDGALRES PWRGAGVAVP IFSIRSNEDL GVGEFLDLKL LVDWAVNSGF
HLVQLLPIND TSVHGMWWDS YPYSSLSVFA LHPLYLRVQA LSDAIPGDIK DEISQAKKQL
DKKDVDYEAS LASKLSIARK IFKLEKDKVL NSSSFKQFLS ENEEWLKPYA AFCFLRDFFE
TSDHSQWGRF SQFSKEKLDK LVSEGTLHHD VICFHYYIQY HLYMQLSEAA AYARKKKVIL
KGDLPIGVDR NSVDTWVYPT LFRMNTATGA PPDYFDKNGQ NWGFPTYNWE EMSKDNYGWW
RARLTQMAKY FTAYRIDHIL GFFRIWELPD HAATGLVGKF RPSIALSQEE LLSEGLWDFD
RMSRPYILQE TLEEKFGSFW TVIAANFLNE YKKQHYEFKE DCNTEKKIIA KLKNSSEKSL
WLEKEDSIRR GLFDLLQNIV LIRDPEDSTK FYPRFNQEDT SSFNDLDEHS KNILRRLYYD
YYFARQENLW RQNALKTLPV LLNSSDMLAC GEDLGLIPAC VHPVMQELGL IGLRIQRMPS
EPNLEFGIPS QYSYMTVCAP SCHDCSTLRA WWEEDGGRRS RFYQTVIGSD DEPPSRCTPE
VANFIVKQHF DAPSMWAIFP LQDLLALKDK YTTRPAKEET INDPTNPKHY WRFRLHVTLD
SLLDDKDIQA TIKELVTSSG RSFPGKVDGA EESGEKLAKV QLNGKP
