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DPH2_MOUSE
ID   DPH2_MOUSE              Reviewed;         489 AA.
AC   Q9CR25; Q8K0L8;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 {ECO:0000305};
DE   AltName: Full=Diphthamide biosynthesis protein 2;
DE   AltName: Full=Diphtheria toxin resistance protein 2 {ECO:0000305};
DE   AltName: Full=MmDph2;
DE   AltName: Full=S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase DPH2 {ECO:0000305};
GN   Name=Dph2; Synonyms=Dph2l2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Cecum, and Embryo;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   FUNCTION, AND INTERACTION WITH DPH1.
RX   PubMed=15485916; DOI=10.1128/mcb.24.21.9487-9497.2004;
RA   Liu S., Milne G.T., Kuremsky J.G., Fink G.R., Leppla S.H.;
RT   "Identification of the proteins required for biosynthesis of diphthamide,
RT   the target of bacterial ADP-ribosylating toxins on translation elongation
RT   factor 2.";
RL   Mol. Cell. Biol. 24:9487-9497(2004).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA   Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of mouse liver.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Kidney, Pancreas, Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [7]
RP   INTERACTION WITH DPH1.
RX   PubMed=21203470; DOI=10.1371/journal.pone.0015753;
RA   Roy V., Ghani K., Caruso M.;
RT   "A dominant-negative approach that prevents diphthamide formation confers
RT   resistance to Pseudomonas exotoxin A and diphtheria toxin.";
RL   PLoS ONE 5:e15753-e15753(2010).
CC   -!- FUNCTION: Required for the first step of diphthamide biosynthesis, a
CC       post-translational modification of histidine which occurs in elongation
CC       factor 2 (PubMed:15485916). DPH1 and DPH2 transfer a 3-amino-3-
CC       carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a
CC       histidine residue, the reaction is assisted by a reduction system
CC       comprising DPH3 and a NADH-dependent reductase (By similarity).
CC       Facilitates the reduction of the catalytic iron-sulfur cluster found in
CC       the DPH1 subunit (By similarity). {ECO:0000250|UniProtKB:P32461,
CC       ECO:0000269|PubMed:15485916}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000250|UniProtKB:P32461};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit. The cluster facilitates the
CC       reduction of the catalytic iron-sulfur cluster in the DPH1 subunit.
CC       {ECO:0000250|UniProtKB:P32461};
CC   -!- PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis.
CC       {ECO:0000305}.
CC   -!- SUBUNIT: Component of the 2-(3-amino-3-carboxypropyl)histidine synthase
CC       complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase (By
CC       similarity). Interacts with DPH1 (PubMed:15485916, PubMed:21203470).
CC       {ECO:0000250|UniProtKB:P32461, ECO:0000269|PubMed:15485916,
CC       ECO:0000269|PubMed:21203470}.
CC   -!- INTERACTION:
CC       Q9CR25; Q5NCQ5: Dph1; NbExp=2; IntAct=EBI-1561134, EBI-1561119;
CC   -!- SIMILARITY: Belongs to the DPH1/DPH2 family. DPH2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AK011199; BAB27462.1; -; mRNA.
DR   EMBL; AK018638; BAB31322.1; -; mRNA.
DR   EMBL; AL627128; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC031124; AAH31124.1; -; mRNA.
DR   CCDS; CCDS18541.1; -.
DR   RefSeq; NP_080620.1; NM_026344.3.
DR   AlphaFoldDB; Q9CR25; -.
DR   SMR; Q9CR25; -.
DR   IntAct; Q9CR25; 1.
DR   STRING; 10090.ENSMUSP00000030265; -.
DR   iPTMnet; Q9CR25; -.
DR   PhosphoSitePlus; Q9CR25; -.
DR   EPD; Q9CR25; -.
DR   MaxQB; Q9CR25; -.
DR   PaxDb; Q9CR25; -.
DR   PeptideAtlas; Q9CR25; -.
DR   PRIDE; Q9CR25; -.
DR   ProteomicsDB; 279764; -.
DR   Antibodypedia; 32411; 401 antibodies from 24 providers.
DR   DNASU; 67728; -.
DR   Ensembl; ENSMUST00000030265; ENSMUSP00000030265; ENSMUSG00000028540.
DR   GeneID; 67728; -.
DR   KEGG; mmu:67728; -.
DR   UCSC; uc008ujd.1; mouse.
DR   CTD; 1802; -.
DR   MGI; MGI:1914978; Dph2.
DR   VEuPathDB; HostDB:ENSMUSG00000028540; -.
DR   eggNOG; KOG2648; Eukaryota.
DR   GeneTree; ENSGT00940000153694; -.
DR   HOGENOM; CLU_015210_0_0_1; -.
DR   InParanoid; Q9CR25; -.
DR   OMA; PFNNMYD; -.
DR   OrthoDB; 750182at2759; -.
DR   PhylomeDB; Q9CR25; -.
DR   TreeFam; TF313832; -.
DR   BRENDA; 2.5.1.108; 3474.
DR   Reactome; R-MMU-5358493; Synthesis of diphthamide-EEF2.
DR   UniPathway; UPA00559; -.
DR   BioGRID-ORCS; 67728; 19 hits in 73 CRISPR screens.
DR   PRO; PR:Q9CR25; -.
DR   Proteomes; UP000000589; Chromosome 4.
DR   RNAct; Q9CR25; protein.
DR   Bgee; ENSMUSG00000028540; Expressed in ear vesicle and 195 other tissues.
DR   ExpressionAtlas; Q9CR25; baseline and differential.
DR   Genevisible; Q9CR25; MM.
DR   GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR   GO; GO:0090560; F:2-(3-amino-3-carboxypropyl)histidine synthase activity; ISO:MGI.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; ISS:UniProtKB.
DR   GO; GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; ISS:UniProtKB.
DR   Gene3D; 3.40.50.11840; -; 1.
DR   Gene3D; 3.40.50.11860; -; 1.
DR   InterPro; IPR010014; DHP2.
DR   InterPro; IPR016435; DPH1/DPH2.
DR   InterPro; IPR042263; DPH1/DPH2_1.
DR   InterPro; IPR042265; DPH1/DPH2_3.
DR   PANTHER; PTHR10762; PTHR10762; 1.
DR   PANTHER; PTHR10762:SF2; PTHR10762:SF2; 1.
DR   Pfam; PF01866; Diphthamide_syn; 1.
DR   SFLD; SFLDS00032; Radical_SAM_3-amino-3-carboxyp; 1.
DR   TIGRFAMs; TIGR00322; diphth2_R; 1.
DR   TIGRFAMs; TIGR00272; DPH2; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Iron; Iron-sulfur; Metal-binding; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..489
FT                   /note="2-(3-amino-3-carboxypropyl)histidine synthase
FT                   subunit 2"
FT                   /id="PRO_0000307890"
FT   BINDING         89
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:P32461"
FT   BINDING         110
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:P32461"
FT   BINDING         341
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250|UniProtKB:P32461"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BQC3"
FT   MOD_RES         7
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BQC3"
FT   MOD_RES         446
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BQC3"
FT   MOD_RES         467
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BQC3"
FT   MOD_RES         488
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BQC3"
FT   CONFLICT        290
FT                   /note="T -> I (in Ref. 3; AAH31124)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   489 AA;  52365 MW;  2D94A8FEDE63DD39 CRC64;
     MESTFSSPAE AALQREAGVP GQFTPPEDLD RVYELERVTK FVCDLGCQRV TLQFPDQLLG
     DAGAVAARLE EVTGAKMFIL GDTAYGSCCV DVLGAEQAGA QALVHFGPAC LSPPASQLPI
     TFVLGQRPVA LELCAKAFEA QNPDPTAPVV LLSEPACAHA LEPLAMLLLP KYQDLLISRP
     ALPLPVGSPS SQPESLERFG RCFPLNPGRR LEEYGAFYVG ASQASSDSSL DPDLSRLLLG
     WTPGRPFFSC CPDTGQTQDQ GAKAGRLRAR RLYLIERARD ARVVGLLAGT LGVARHREAL
     AHLRKLTEAA GKRSYVLAVG KPTPAKLANF PEMDVFVLLA CPLGALAPQP SGGFFRPVLT
     PCELEAACNP AWPPPGLAPH LTHYAELLPG SPFHVPLPPP ESELWDTPDV SLISGELRPP
     PPWKSSDDTR CSALIPRPQL ELAESSPAAS FLSSRNWQGL EPRLGQTPVK EAVRGRRGIA
     IAYEDEGSS
 
 
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