ID   DPH6_DANRE              Reviewed;         255 AA.
AC   A2RV01; Q5EAS1;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Diphthine--ammonia ligase;
DE            EC=6.3.1.14;
DE   AltName: Full=ATP-binding domain-containing protein 4;
DE   AltName: Full=Diphthamide synthase;
DE   AltName: Full=Diphthamide synthetase;
DE   AltName: Full=Protein DPH6 homolog;
GN   Name=dph6; Synonyms=atpbd4; ORFNames=zgc:110758;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Embryo, and Eye;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Amidase that catalyzes the last step of diphthamide
CC       biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists
CC       in the conversion of an L-histidine residue in the translation
CC       elongation factor eEF-2 (EEF2) to diphthamide (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + diphthine-[translation elongation factor 2] + NH4(+) =
CC         AMP + diphosphate + diphthamide-[translation elongation factor 2] +
CC         H(+); Xref=Rhea:RHEA:19753, Rhea:RHEA-COMP:10172, Rhea:RHEA-
CC         COMP:10174, ChEBI:CHEBI:15378, ChEBI:CHEBI:16692, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:82696,
CC         ChEBI:CHEBI:456215; EC=6.3.1.14;
CC   -!- PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=A2RV01-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=A2RV01-2; Sequence=VSP_024127;
CC   -!- SIMILARITY: Belongs to the Diphthine--ammonia ligase family.
CC       {ECO:0000305}.
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DR   EMBL; BC090272; AAH90272.1; -; mRNA.
DR   EMBL; BC133118; AAI33119.1; -; mRNA.
DR   RefSeq; NP_001013308.2; NM_001013290.2. [A2RV01-1]
DR   AlphaFoldDB; A2RV01; -.
DR   SMR; A2RV01; -.
DR   STRING; 7955.ENSDARP00000106089; -.
DR   PaxDb; A2RV01; -.
DR   PeptideAtlas; A2RV01; -.
DR   Ensembl; ENSDART00000156774; ENSDARP00000129691; ENSDARG00000042839. [A2RV01-1]
DR   GeneID; 503603; -.
DR   KEGG; dre:503603; -.
DR   CTD; 89978; -.
DR   ZFIN; ZDB-GENE-050227-15; dph6.
DR   eggNOG; KOG2316; Eukaryota.
DR   eggNOG; KOG2317; Eukaryota.
DR   GeneTree; ENSGT00420000029820; -.
DR   HOGENOM; CLU_010289_0_0_1; -.
DR   InParanoid; A2RV01; -.
DR   OMA; NYALYWA; -.
DR   OrthoDB; 707303at2759; -.
DR   PhylomeDB; A2RV01; -.
DR   UniPathway; UPA00559; -.
DR   PRO; PR:A2RV01; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 17.
DR   Bgee; ENSDARG00000042839; Expressed in liver and 22 other tissues.
DR   ExpressionAtlas; A2RV01; baseline.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0017178; F:diphthine-ammonia ligase activity; IBA:GO_Central.
DR   GO; GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IBA:GO_Central.
DR   CDD; cd01994; Alpha_ANH_like_IV; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR002761; Diphthami_syn_dom.
DR   InterPro; IPR030662; DPH6/MJ0570.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR12196; PTHR12196; 1.
DR   Pfam; PF01902; Diphthami_syn_2; 1.
DR   PIRSF; PIRSF039123; Diphthamide_synthase; 1.
DR   TIGRFAMs; TIGR00290; MJ0570_dom; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ATP-binding; Ligase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..255
FT                   /note="Diphthine--ammonia ligase"
FT                   /id="PRO_0000282400"
FT   VAR_SEQ         39
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_024127"
FT   CONFLICT        184
FT                   /note="L -> P (in Ref. 1; AAH90272)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   255 AA;  28401 MW;  131561D4D4D5196A CRC64;
     MRVVGLISGG KDSCFNMLQC VSAGHSIVAL ANLRPADHAA SDELDSYMYQ TVGHQAVDLI
     AEAMGLPLYR RTIEGSSVHI DREYSPTDGD EVEDLYQLLK HVKEEMHVDG VSVGAILSDY
     QRVRVENVCA RLQLQPLAYL WRRDQAALLS EMISSGLHAI LIKVAAFGLH PDKHLGKSLA
     EMELYLHELS EKYGVHICGE GGEYETFTLD CPLFKKKIII DATETVIHSD DAFAPVGFLR
     FTKMHTEDKT EVRTL