ID   DPH7_DICDI              Reviewed;         367 AA.
AC   Q55C80;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Diphthine methyltransferase homolog;
DE            EC=3.1.1.97;
DE   AltName: Full=Diphthamide biosynthesis protein 7 homolog;
DE   AltName: Full=WD repeat-containing protein 85 homolog;
GN   Name=wdr85; ORFNames=DDB_G0270178;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Catalyzes the demethylation of diphthine methyl ester to form
CC       diphthine, an intermediate diphthamide biosynthesis, a post-
CC       translational modification of histidine which occurs in translation
CC       elongation factor 2 (efbA). {ECO:0000250|UniProtKB:Q9BTV6}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphthine methyl ester-[translation elongation factor 2] + H2O
CC         = diphthine-[translation elongation factor 2] + H(+) + methanol;
CC         Xref=Rhea:RHEA:42656, Rhea:RHEA-COMP:10172, Rhea:RHEA-COMP:10173,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790,
CC         ChEBI:CHEBI:79005, ChEBI:CHEBI:82696; EC=3.1.1.97;
CC         Evidence={ECO:0000250|UniProtKB:P38332};
CC   -!- PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis.
CC   -!- SIMILARITY: Belongs to the DPH7 family. {ECO:0000305}.
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DR   EMBL; AAFI02000005; EAL72439.1; -; Genomic_DNA.
DR   RefSeq; XP_646601.1; XM_641509.1.
DR   AlphaFoldDB; Q55C80; -.
DR   SMR; Q55C80; -.
DR   STRING; 44689.DDB0267165; -.
DR   PaxDb; Q55C80; -.
DR   EnsemblProtists; EAL72439; EAL72439; DDB_G0270178.
DR   GeneID; 8617573; -.
DR   KEGG; ddi:DDB_G0270178; -.
DR   dictyBase; DDB_G0270178; wdr85.
DR   eggNOG; KOG0280; Eukaryota.
DR   HOGENOM; CLU_036100_2_0_1; -.
DR   InParanoid; Q55C80; -.
DR   OMA; LDMKWLP; -.
DR   PhylomeDB; Q55C80; -.
DR   UniPathway; UPA00559; -.
DR   PRO; PR:Q55C80; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0061685; F:diphthine methylesterase activity; IBA:GO_Central.
DR   GO; GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF00400; WD40; 1.
DR   SMART; SM00320; WD40; 5.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..367
FT                   /note="Diphthine methyltransferase homolog"
FT                   /id="PRO_0000330844"
FT   REPEAT          84..124
FT                   /note="WD 1"
FT   REPEAT          132..173
FT                   /note="WD 2"
FT   REPEAT          180..220
FT                   /note="WD 3"
FT   REPEAT          234..274
FT                   /note="WD 4"
SQ   SEQUENCE   367 AA;  42561 MW;  53D22E2A9F62EC96 CRC64;
     MTEIIKNTKY LDYTSDSVEF YPFNNNIFVC GTYEIEKGDT EYKERRKGKL YLFEIEEEQQ
     QKENDNNNEN NNNNKLFKEI QNINFNSGIL DMKWNNNKDR ILGVVMSKGE LNIYQYDEVE
     KKLELKSSTE ISLSNDILSL SLDWNKSGDK LICSFSDGNI GLFKVTKDYS KVTEEKRWKA
     HDYEAWICAF NYHDESIVFS GGDDCKFKIW DLNQLLNHND DDIGIPPTPK FTKRCDMGVT
     SIHCHPTIEN LIAVGSYDEC LRIWDLKSLK QPIITTDSLG GGIWRIKWHP FQKNKLVTAC
     MGGGFHILST DPENINDFST LQIEQSYNGP HKSIAYGVDW SFNKNNFDKQ FIGCCSFYDK
     CLSIWIP