ID   DPIA_ECO57              Reviewed;         226 AA.
AC   P0AEF6; Q54149;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Transcriptional regulatory protein DpiA;
DE   AltName: Full=Destabilizer of plasmid inheritance;
GN   Name=dpiA; Synonyms=citB; OrderedLocusNames=Z0765, ECs0659;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Member of the two-component regulatory system DpiA/DpiB,
CC       which is essential for expression of citrate-specific fermentation
CC       genes and genes involved in plasmid inheritance. Could be involved in
CC       response to both the presence of citrate and external redox conditions
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated and activated by DpiB. {ECO:0000250}.
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DR   EMBL; AE005174; AAG54955.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34082.1; -; Genomic_DNA.
DR   PIR; C90711; C90711.
DR   PIR; G85561; G85561.
DR   RefSeq; NP_308686.1; NC_002695.1.
DR   RefSeq; WP_000126500.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AEF6; -.
DR   SMR; P0AEF6; -.
DR   STRING; 155864.EDL933_0693; -.
DR   PRIDE; P0AEF6; -.
DR   EnsemblBacteria; AAG54955; AAG54955; Z0765.
DR   EnsemblBacteria; BAB34082; BAB34082; ECs_0659.
DR   GeneID; 66671105; -.
DR   GeneID; 917019; -.
DR   KEGG; ece:Z0765; -.
DR   KEGG; ecs:ECs_0659; -.
DR   PATRIC; fig|386585.9.peg.770; -.
DR   eggNOG; COG4565; Bacteria.
DR   HOGENOM; CLU_000445_39_0_6; -.
DR   OMA; MRHGALQ; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR024187; Sig_transdc_resp-reg_cit/mal.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR028141; Transcriptional_reg_dom.
DR   PANTHER; PTHR45526; PTHR45526; 1.
DR   Pfam; PF12431; CitT; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PIRSF; PIRSF006171; RR_citrat_malat; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..226
FT                   /note="Transcriptional regulatory protein DpiA"
FT                   /id="PRO_0000081069"
FT   DOMAIN          6..122
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        180..199
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         57
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   226 AA;  25453 MW;  2A47279EED145743 CRC64;
     MTAPLTLLIV EDETPLAEMH AEYIRHIPGF SQILLAGNLA QARMMIERFK PGLILLDNYL
     PDGRGINLLH ELVQAHYPGD VVFTTAASDM ETVSEAVRCG VFDYLIKPIA YERLGQTLTR
     FRQRKHMLES IDSASQKQID EMFNAYARGE PKDELPTGID PLTLNAVRKL FKEPGVQHTA
     ETVAQALTIS RTTARRYLEY CASRHLIIAE IVHGKVGRPQ RIYHSG