DPM2_MOUSE
ID DPM2_MOUSE Reviewed; 84 AA.
AC Q9Z324;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Dolichol phosphate-mannose biosynthesis regulatory protein {ECO:0000305};
DE AltName: Full=Dolichol-phosphate mannose synthase subunit 2;
DE Short=DPM synthase subunit 2;
GN Name=Dpm2 {ECO:0000312|MGI:MGI:1330238};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9724629; DOI=10.1093/emboj/17.17.4920;
RA Maeda Y., Tomita S., Watanabe R., Ohishi K., Kinoshita T.;
RT "DPM2 regulates biosynthesis of dolichol phosphate-mannose in mammalian
RT cells: correct subcellular localization and stabilization of DPM1, and
RT binding of dolichol phosphate.";
RL EMBO J. 17:4920-4929(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=129; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Regulates the biosynthesis of dolichol phosphate-mannose.
CC Regulatory subunit of the dolichol-phosphate mannose (DPM) synthase
CC complex; essential for the ER localization and stable expression of
CC DPM1. Part of the glycosylphosphatidylinositol-N-
CC acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the
CC transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to
CC phosphatidylinositol and participates in the first step of GPI
CC biosynthesis. May act by regulating the GPI-GNT complex.
CC {ECO:0000250|UniProtKB:O94777}.
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC {ECO:0000250|UniProtKB:O94777}.
CC -!- SUBUNIT: Component of the dolichol-phosphate mannose (DPM) synthase
CC complex composed of DPM1, DPM2 and DPM3; in the complex interacts
CC directly with DPM3. Component of the glycosylphosphatidylinositol-N-
CC acetylglucosaminyltransferase (GPI-GnT) complex composed at least by
CC PIGA, PIGC, PIGH, PIGP, PIGQ, PIGY and DPM2. Interacts with PIGA, PIGC
CC and PIGQ. {ECO:0000250|UniProtKB:O94777}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein.
CC -!- SIMILARITY: Belongs to the DPM2 family. {ECO:0000305}.
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DR EMBL; AB013360; BAA33973.1; -; mRNA.
DR EMBL; BC008256; AAH08256.1; -; mRNA.
DR CCDS; CCDS38104.1; -.
DR RefSeq; NP_034203.1; NM_010073.2.
DR AlphaFoldDB; Q9Z324; -.
DR BioGRID; 199299; 1.
DR IntAct; Q9Z324; 1.
DR MINT; Q9Z324; -.
DR STRING; 10090.ENSMUSP00000028151; -.
DR PaxDb; Q9Z324; -.
DR PRIDE; Q9Z324; -.
DR TopDownProteomics; Q9Z324; -.
DR Antibodypedia; 44831; 43 antibodies from 16 providers.
DR DNASU; 13481; -.
DR Ensembl; ENSMUST00000028151; ENSMUSP00000028151; ENSMUSG00000026810.
DR GeneID; 13481; -.
DR KEGG; mmu:13481; -.
DR UCSC; uc008jfu.1; mouse.
DR CTD; 8818; -.
DR MGI; MGI:1330238; Dpm2.
DR VEuPathDB; HostDB:ENSMUSG00000026810; -.
DR eggNOG; KOG3488; Eukaryota.
DR GeneTree; ENSGT00390000001098; -.
DR HOGENOM; CLU_150144_2_1_1; -.
DR InParanoid; Q9Z324; -.
DR OMA; YVMLKSQ; -.
DR OrthoDB; 1605216at2759; -.
DR PhylomeDB; Q9Z324; -.
DR TreeFam; TF300257; -.
DR Reactome; R-MMU-162699; Synthesis of dolichyl-phosphate mannose.
DR Reactome; R-MMU-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00378; -.
DR BioGRID-ORCS; 13481; 15 hits in 79 CRISPR screens.
DR ChiTaRS; Dpm2; mouse.
DR PRO; PR:Q9Z324; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9Z324; protein.
DR Bgee; ENSMUSG00000026810; Expressed in embryonic brain and 265 other tissues.
DR ExpressionAtlas; Q9Z324; baseline and differential.
DR Genevisible; Q9Z324; MM.
DR GO; GO:0033185; C:dolichol-phosphate-mannose synthase complex; ISO:MGI.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:MGI.
DR GO; GO:0000506; C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; ISS:UniProtKB.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; ISM:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0004582; F:dolichyl-phosphate beta-D-mannosyltransferase activity; ISO:MGI.
DR GO; GO:0008047; F:enzyme activator activity; ISO:MGI.
DR GO; GO:0030234; F:enzyme regulator activity; ISO:MGI.
DR GO; GO:0019348; P:dolichol metabolic process; ISO:MGI.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; ISS:UniProtKB.
DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR GO; GO:0031647; P:regulation of protein stability; ISO:MGI.
DR InterPro; IPR009914; DPM2.
DR PANTHER; PTHR15039; PTHR15039; 1.
DR Pfam; PF07297; DPM2; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..84
FT /note="Dolichol phosphate-mannose biosynthesis regulatory
FT protein"
FT /id="PRO_0000220874"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 84 AA; 9376 MW; 345C9AC32045A300 CRC64;
MATGTDQAVG FGLVAVSLII FTYYTTWVIL LPFIDSQHVI HKYFLPRAYA VLLPLAAGLL
LLLFVGLFIT YVMLKSQKIT KKAQ
