ID   DPO1_AQUAE              Reviewed;         574 AA.
AC   O67779;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=DNA polymerase I;
DE            Short=POL I;
DE            EC=2.7.7.7;
GN   Name=polA; OrderedLocusNames=aq_1967;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
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DR   EMBL; AE000657; AAC07735.1; -; Genomic_DNA.
DR   PIR; G70468; G70468.
DR   RefSeq; NP_214348.1; NC_000918.1.
DR   RefSeq; WP_010881284.1; NC_000918.1.
DR   AlphaFoldDB; O67779; -.
DR   SMR; O67779; -.
DR   STRING; 224324.aq_1967; -.
DR   DNASU; 1193676; -.
DR   EnsemblBacteria; AAC07735; AAC07735; aq_1967.
DR   KEGG; aae:aq_1967; -.
DR   PATRIC; fig|224324.8.peg.1518; -.
DR   eggNOG; COG0749; Bacteria.
DR   HOGENOM; CLU_004675_2_6_0; -.
DR   InParanoid; O67779; -.
DR   OMA; EFKQIGA; -.
DR   OrthoDB; 1220182at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR   GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR   InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR002298; DNA_polymerase_A.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR10133; PTHR10133; 1.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   Pfam; PF01612; DNA_pol_A_exo1; 1.
DR   PRINTS; PR00868; DNAPOLI.
DR   SMART; SM00474; 35EXOc; 1.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; DNA-binding;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..574
FT                   /note="DNA polymerase I"
FT                   /id="PRO_0000101232"
FT   DOMAIN          4..161
FT                   /note="3'-5' exonuclease"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   574 AA;  65833 MW;  69F3254EAE0E1746 CRC64;
     MDFEYVTGEE GLKKAIKRLE NSPYLYLDTE TTGDRIRLVQ IGDEENTYVI DLYEIQDIEP
     LRKLINERGI VGHNLKFDLK YLYRYGIFPS ATFDTMIASY LLGYERHSLN HIVSNLLGYS
     MDKSYQTSDW GASVLSDAQL KYAANDVIVL RELFPKMRDM LNELDAERGE ELLKTRTAKI
     FDLKSPVAIV EMAFVREVAK LEINGFPVDV EELTNKLKAV ERETQKRIQE FYIKYRVDPL
     SPKQLASLLT KKFKLNLPKT PKGNVSTDDK ALTSYQDVEP VKLVLEIRKL KKIADKLKEL
     KEHLKNGRVY PEFKQIGAVT GRMSSAHPNI QNIHRDMRGI FKAEEGNTFV ISDFSQIELR
     IAAEYVKDPL MLDAFKKGKD MHRYTASVVL GKKEEEITKE ERQLAKAINF GLIYGISAKG
     LAEYAKLGYG VEISLEEAQV LRERFFKNFK AFKEWHDRVK KELKEKGEVK GHTLLGRRFS
     ANTFNDAVNY PIQGTGADLL KLAVLLFDAN LQKKGIDAKL VNLVHDEIVV ECEKEKAEEV
     KEILEKSMKT AGKIILKEVP VEVESVINER WTKD