ID   DPO1_BORBU              Reviewed;         908 AA.
AC   O51498;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=DNA polymerase I;
DE            Short=POL I;
DE            EC=2.7.7.7;
GN   Name=polA; OrderedLocusNames=BB_0548;
OS   Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS   (Borrelia burgdorferi).
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=224326;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=9403685; DOI=10.1038/37551;
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA   Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA   Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA   van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA   Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA   Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA   Smith H.O., Venter J.C.;
RT   "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL   Nature 390:580-586(1997).
CC   -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC       exhibits 3'-5' and 5'-3' exonuclease activity. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
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DR   EMBL; AE000783; AAC66909.1; -; Genomic_DNA.
DR   PIR; C70168; C70168.
DR   RefSeq; NP_212682.1; NC_001318.1.
DR   RefSeq; WP_010889763.1; NC_001318.1.
DR   AlphaFoldDB; O51498; -.
DR   SMR; O51498; -.
DR   STRING; 224326.BB_0548; -.
DR   DNASU; 1195395; -.
DR   EnsemblBacteria; AAC66909; AAC66909; BB_0548.
DR   KEGG; bbu:BB_0548; -.
DR   PATRIC; fig|224326.49.peg.939; -.
DR   HOGENOM; CLU_004675_0_0_12; -.
DR   OMA; NRPPMPD; -.
DR   Proteomes; UP000001807; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR   CDD; cd09898; H3TH_53EXO; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR   InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR   InterPro; IPR002421; 5-3_exonuclease.
DR   InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR   InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR020045; DNA_polI_H3TH.
DR   InterPro; IPR018320; DNA_polymerase_1.
DR   InterPro; IPR002298; DNA_polymerase_A.
DR   InterPro; IPR008918; HhH2.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR10133; PTHR10133; 1.
DR   Pfam; PF01367; 5_3_exonuc; 1.
DR   Pfam; PF02739; 5_3_exonuc_N; 1.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   Pfam; PF01612; DNA_pol_A_exo1; 1.
DR   PRINTS; PR00868; DNAPOLI.
DR   SMART; SM00474; 35EXOc; 1.
DR   SMART; SM00475; 53EXOc; 1.
DR   SMART; SM00279; HhH2; 1.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF47807; SSF47807; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00593; pola; 1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; DNA-binding;
KW   DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..908
FT                   /note="DNA polymerase I"
FT                   /id="PRO_0000101236"
FT   DOMAIN          1..318
FT                   /note="5'-3' exonuclease"
FT   DOMAIN          319..531
FT                   /note="3'-5' exonuclease"
FT   REGION          532..908
FT                   /note="Polymerase"
SQ   SEQUENCE   908 AA;  105504 MW;  B58512AEB0DCBD94 CRC64;
     MKELYLIDAL NIIFRNYHVM KNYPLLNTQG ENVNAFIGFF KTLFFIIKEK NPEHLIITFD
     SEVPTFRKQK YPSYKATRDL PPDDLIPQIG WIKEGLLKAK IPIFEMEGYE ADDLLASFAK
     KAAKNNYLTY IISPDKDLLQ TMSEYVKILK IENNSFIEMD NEYVTKKFGV NSFQIKDYLA
     IVGDRSDNIP GIKGIGAKGA ANLLREFKTL DGIYSNLEII NKKHRELLIK EKENAFLSYE
     LVSLEENLKI PEIENFALKN FSEEIISLFE KHSAIALIKT YKKDILKQEK ENADQKSLFK
     QEPTTNSLDD INTIDTENVK YRSITTKIEL DDLIESLKKA KYISIDTETS SLDTYTAKLI
     GISISFKEFE GYYIPIEAKG KIYIEKNYII QKFNNLFESN PKIIGQNYKF DYKILKNNGF
     NPIPPYFDTM IAAYLIDTNS KVSLDFLAEK YLMHKNIKYE DVIQKNDNFA NISLEMATSY
     SSEDADITFR LFNIFTKKLK EDKLDKLMHE IEMPFNKVII EMEENGIYLD KEYLKEYGKE
     LGKELEAIEN EIIKSIGIDF NLNSPKQMHE ILFEKLNLKL PEKMKKDSTD IKVLESLREQ
     HESIENLIKY RQIAKLKSTY TDNLIELINY KTNRLHTSFI QTKTATGRIT SINPNLQNIP
     IKDEKGRKIR KAFKPENGNI FISADYSQIE LAILAHLSQD EVLIKAFENN KDIHTETASK
     LFKIEEKEIT PNLRRIAKSI NFGIIYRMSD FRLAKELGIT KEEAKGFINS YFDSYPKIKE
     FIINQINFVR NAGYSETILK RRRYIKEINS NNYLERSAAE RIAINSIIQG SAADIMKIAM
     VKVFNEFKSK KMESKILLQV HDEMLIESPI EEENEVKKIL KIMMETAYTL NLPLRANIET
     GKSWGEIH