DPO1_CALBD
ID DPO1_CALBD Reviewed; 850 AA.
AC Q59156; B9MS85;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 14-APR-2009, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=DNA polymerase I;
DE Short=Pol I;
DE EC=2.7.7.7;
GN Name=polA; OrderedLocusNames=Athe_1441;
OS Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320)
OS (Anaerocellum thermophilum).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacterales Family III. Incertae Sedis; Caldicellulosiruptor.
OX NCBI_TaxID=521460;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Bolchakova E.V., Novikov A.A., Zverlov V.V., Galina V.,
RA Velikodvorskaya G.V.;
RL Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1888 / DSM 6725 / Z-1320;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA Goodwin L., Pitluck S., Sims D., Meincke L., Brettin T., Detter J.C.,
RA Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA Kataeva I., Adams M.W.W.;
RT "Complete sequence of chromosome of Anaerocellum thermophilum DSM 6725.";
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 3'-5' and 5'-3' exonuclease activity. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
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DR EMBL; X98575; CAA67184.1; -; Genomic_DNA.
DR EMBL; CP001393; ACM60539.1; -; Genomic_DNA.
DR RefSeq; WP_015907902.1; NC_012034.1.
DR AlphaFoldDB; Q59156; -.
DR SMR; Q59156; -.
DR STRING; 521460.Athe_1441; -.
DR PRIDE; Q59156; -.
DR EnsemblBacteria; ACM60539; ACM60539; Athe_1441.
DR GeneID; 31772786; -.
DR KEGG; ate:Athe_1441; -.
DR eggNOG; COG0258; Bacteria.
DR eggNOG; COG0749; Bacteria.
DR HOGENOM; CLU_004675_0_0_9; -.
DR OMA; NRPPMPD; -.
DR OrthoDB; 1220182at2; -.
DR Proteomes; UP000007723; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR CDD; cd09898; H3TH_53EXO; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10133; PTHR10133; 2.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; SSF47807; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
DR TIGRFAMs; TIGR00593; pola; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; DNA replication; DNA-binding;
KW DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease;
KW Nucleotidyltransferase; Transferase.
FT CHAIN 1..850
FT /note="DNA polymerase I"
FT /id="PRO_0000101231"
FT DOMAIN 1..288
FT /note="5'-3' exonuclease"
FT REGION 470..850
FT /note="Polymerase"
FT /evidence="ECO:0000250"
FT CONFLICT 451
FT /note="N -> S (in Ref. 1; CAA67184)"
FT /evidence="ECO:0000305"
FT CONFLICT 774
FT /note="A -> P (in Ref. 1; CAA67184)"
FT /evidence="ECO:0000305"
FT CONFLICT 831
FT /note="A -> R (in Ref. 1; CAA67184)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 850 AA; 98130 MW; AAEAC6DB4525A7FD CRC64;
MKLVIFDGNS ILYRAFFALP ELTTSNNIPT NAIYGFVNVI LKYLEQEKPD YVAVAFDKRG
REARKSEYEE YKANRKPMPD NLQVQIPYVR EILYAFNIPI IEFEGYEADD VIGSLVNQFK
NTGLDIVIIT GDRDTLQLLD KNVVVKIVST KFDKTVEDLY TVENVKEKYG VWANQVPDYK
ALVGDQSDNI PGVKGIGEKS AQKLLEEYSS LEEIYQNLDK IKSSIREKLE AGKDMAFLSK
RLATIVCDLP LNVKLEDLRT KEWNKERLYE ILVQLEFKSI IKRLGLSEVV QFEFVQQRTD
IPDVEQKELE SISQIRSKEI PLMFVQGEKC FYLYDQESNT VFITSNKLLI EEILKSDTVK
IMYDLKNIFH QLNLEDTNNI KNCEDVMIAS YVLDSTRSSY ELETLFVSYL NTDIEAVKKD
KKIVSVVLLK RLWDELLRLI DLNSCQFLYE NIERPLIPVL YEMEKTGFKV DRDALIQYTK
EIENKILKLE TQIYQIAGEW FNINSPKQLS YILFEKLKLP VIKKTKTGYS TDAEVLEELF
DKHEIVPLIL DYRMYTKILT TYCQGLLQAI NPSSGRVHTT FIQTGTATGR LASSDPNLQN
IPVKYDEGKL IRKVFVPEGG HVLIDADYSQ IELRILAHIS EDERLISAFK NNVDIHSQTA
AEVFGVDIAD VTPEMRSQAK AVNFGIVYGI SDYGLARDIK ISRKEAAEFI NKYFERYPKV
KEYLDNTVKF ARDNGFVLTL FNRKRYIKDI KSTNRNLRGY AERIAMNSPI QGSAADIMKL
AMIKVYQKLK ENNLKSKIIL QVHDELLIEA PYEEKDIVKE IVKREMENAV ALKVPLVVEV
KEGLNWYETK
