DPO1_KLULA
ID DPO1_KLULA Reviewed; 995 AA.
AC P09804;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=DNA polymerase;
DE EC=2.7.7.7;
GN Name=RF1;
OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OG Plasmid pGKl-1.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX NCBI_TaxID=284590;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 76492 / CBS 2359/152 / CLIB 210;
RX DOI=10.1007/BF00436963;
RA Sor F., Fukuhara H.;
RT "Structure of a linear plasmid of the yeast Kluyveromyces lactis; compact
RT organization of the killer genome.";
RL Curr. Genet. 9:147-155(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX PubMed=6473099; DOI=10.1093/nar/12.15.6011;
RA Stark M.J.R., Mileham A.J., Romanos M.A., Boyd A.;
RT "Nucleotide sequence and transcription analysis of a linear DNA plasmid
RT associated with the killer character of the yeast Kluyveromyces lactis.";
RL Nucleic Acids Res. 12:6011-6030(1984).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 52735 / 2105-1D;
RX PubMed=3340559; DOI=10.1093/nar/16.2.771;
RA Stark M.J.R.;
RT "Resolution of sequence discrepancies in the ORF1 region of the
RT Kluyveromyces lactis plasmid k1.";
RL Nucleic Acids Res. 16:771-771(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 52735 / 2105-1D;
RX PubMed=6387625; DOI=10.1093/nar/12.19.7581;
RA Hishinuma F., Nakamura K., Hirai K., Nishizawa R., Gunge N., Maeda T.;
RT "Cloning and nucleotide sequences of the linear DNA killer plasmids from
RT yeast.";
RL Nucleic Acids Res. 12:7581-7597(1984).
RN [5]
RP POSSIBLE IDENTIFICATION OF PROTEIN.
RX PubMed=3684586; DOI=10.1093/nar/15.21.9088;
RA Jung G., Leavitt M.C., Ito J.;
RT "Yeast killer plasmid pGKL1 encodes a DNA polymerase belonging to the
RT family B DNA polymerases.";
RL Nucleic Acids Res. 15:9088-9088(1987).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- MISCELLANEOUS: This DNA polymerase requires a protein as a primer.
CC -!- MISCELLANEOUS: The presence of the two linear plasmids, termed pGKl-1
CC and pGKl-2, in strains of Kluyveromyces lactis confers the killer
CC phenotype, i.e. production of toxin and resistance to it, to the host
CC cell.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA30136.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X07127; CAA30136.1; ALT_INIT; Genomic_DNA.
DR EMBL; X01095; CAA25568.1; -; Genomic_DNA.
DR EMBL; X00762; CAA25333.1; -; Genomic_DNA.
DR PIR; S07353; S07353.
DR AlphaFoldDB; P09804; -.
DR InParanoid; P09804; -.
DR Proteomes; UP000000598; Plasmid pGKL1.
DR GO; GO:0110165; C:cellular anatomical entity; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR Gene3D; 3.90.1600.10; -; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR004868; DNA-dir_DNA_pol_B_mt/vir.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR Pfam; PF03175; DNA_pol_B_2; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA-binding; DNA-directed DNA polymerase;
KW Nucleotidyltransferase; Plasmid; Reference proteome; Transferase.
FT CHAIN 1..995
FT /note="DNA polymerase"
FT /id="PRO_0000046548"
FT CONFLICT 339
FT /note="R -> K (in Ref. 1; CAA30136)"
FT /evidence="ECO:0000305"
FT CONFLICT 504
FT /note="P -> S (in Ref. 1; CAA30136)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 995 AA; 116346 MW; C78AFFECE36A5F70 CRC64;
MNYIINLKMD YKDKALNDLR NVYADFDSLP LDFRQILIKD RATLLQKEDV EKKILERQED
AKKYAEYLKQ SEIPERISLP NIKRHKGVSI SFEETSEDMV LEPRPFIFDG LNIRCFRRET
IFSLKNKILN MVKESSSFKN VSRQSVSFMY FKIFNKGKVI ASTKSVNIYE DKIDERLEDL
CNNFDDVLKK IIDVTYGYES LFVSETYSYV IFYAKSIYFP QPRCVNNWGN NIPNILTFDS
FKLFTANKNN VSCIKQCSRF LWQKDFNTLE EMIEYKNGNI CIVTPQLHIN DVRDIKSFND
IRLYSESPIK TFSVIDNTIT YLFYFKEHLG VIFNITKSRH DRRVTKFSPL SKFSDVKNIT
VCFDIESYFD PEKESNQVNI PFICCASIIY NKVIGNIVDF EGRDCVAQMI EYVVDICGEL
NISSVELIAH NGGGYDFHYI LSSMYNPAAI KNILIRNNSF ISFNFAHDGV KFSVKDSYSF
LLCSLANASK AFLNEETFKK TDFPHHDLKT ADDLYKVYKE WSSVNTEINH VVEKEKLLIT
SEHIVNFTKN DKSKTLIEWS KDYCRNDVLV LSKVWLEFKN AVEDIFNCEL VDQTMTLAGL
SYKLFQANMP FDVELRHPNK EDYFNMREAL IGGRCISVNG IYKDVLCLDV KSLYPASMAF
YDQPYGSFKR VSSRPKDELG IYYVRVTPNR NNKSNFFPIR SHNKITYNNF EESTYIAWYT
NVDIDIGLSE GHNIEYIPFD SYGNIGYSWS KKGKIFEKYI KDVLYKLKIK YEKQNNKVKR
NVIKIIMNSL WGKFAQKWVN FEYFIKSEDD IDFESEEAYK IWDTDFMLIK KIKESTYSSK
PIQNGVFTLS WARYHMKSIW DAGAKEGAEC IYSDTDSIFV HKEHFKKNAK FMLNGLKVPI
IGSEVGQLEL ECEFDKLLCA GKKQYMGFYT YFQDGKPCIK EKKRFKGIPS NYIIPELYAH
LLSGADKEAK IQFLKFRREW GSVKGYIENK TVKAT
