ID   DPO1_MYCLE              Reviewed;         911 AA.
AC   P46835;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=DNA polymerase I;
DE            Short=POL I;
DE            EC=2.7.7.7;
GN   Name=polA; OrderedLocusNames=ML1381;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7476188; DOI=10.1111/j.1365-2958.1995.tb02317.x;
RA   Fsihi H., Cole S.T.;
RT   "The Mycobacterium leprae genome: systematic sequence analysis identifies
RT   key catabolic enzymes, ATP-dependent transport systems and a novel polA
RT   locus associated with genomic variability.";
RL   Mol. Microbiol. 16:909-919(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Smith D.R., Robison K.;
RL   Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC       exhibits 3'-5' and 5'-3' exonuclease activity. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: Single-chain monomer with multiple functions.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA50927.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; Z46257; CAA86364.1; -; Genomic_DNA.
DR   EMBL; U00021; AAA50927.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AL583921; CAC31762.1; -; Genomic_DNA.
DR   PIR; S77659; S77659.
DR   RefSeq; NP_301982.1; NC_002677.1.
DR   RefSeq; WP_049769942.1; NC_002677.1.
DR   AlphaFoldDB; P46835; -.
DR   SMR; P46835; -.
DR   STRING; 272631.ML1381; -.
DR   PRIDE; P46835; -.
DR   EnsemblBacteria; CAC31762; CAC31762; CAC31762.
DR   KEGG; mle:ML1381; -.
DR   PATRIC; fig|272631.5.peg.2560; -.
DR   Leproma; ML1381; -.
DR   eggNOG; COG0258; Bacteria.
DR   eggNOG; COG0749; Bacteria.
DR   HOGENOM; CLU_004675_0_0_11; -.
DR   OMA; NRPPMPD; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR   CDD; cd09898; H3TH_53EXO; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR   InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR   InterPro; IPR002421; 5-3_exonuclease.
DR   InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR   InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR020045; DNA_polI_H3TH.
DR   InterPro; IPR018320; DNA_polymerase_1.
DR   InterPro; IPR002298; DNA_polymerase_A.
DR   InterPro; IPR008918; HhH2.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR10133; PTHR10133; 1.
DR   Pfam; PF01367; 5_3_exonuc; 1.
DR   Pfam; PF02739; 5_3_exonuc_N; 1.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   PRINTS; PR00868; DNAPOLI.
DR   SMART; SM00474; 35EXOc; 1.
DR   SMART; SM00475; 53EXOc; 1.
DR   SMART; SM00279; HhH2; 1.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF47807; SSF47807; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00593; pola; 1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; DNA-binding;
KW   DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..911
FT                   /note="DNA polymerase I"
FT                   /id="PRO_0000101246"
FT   DOMAIN          186..280
FT                   /note="5'-3' exonuclease"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          320..497
FT                   /note="3'-5' exonuclease"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   911 AA;  99792 MW;  12B4EA18B2BC9864 CRC64;
     MSAAMTAEVC EDHTKPMLML LDGNSLAFRA FYALPTENFK TRGGLTTNAV YGFTAMLINL
     LREEAPTHIA AAFDVSRKTF RSECYAGYKA NRSSIPAEFH GQIDITKEVL GALGITVFAE
     AGFEADDLIA TLATQAENEG YRVLVVTGDR DALQLVSNDV TVLYPRKGVS ELTRFTPEAV
     IEKYGVTPAQ YPDLAALRGD PSDNLPGIPG VGEKTAAKWI VDYGSLQGLV DNVESVRGKV
     GEALRTHLAS VVRNRELTEL VKDVPLVQTS DTLRLQPWDR DRIHRLFDNL EFRVLRDRLF
     EALAAAGERV PEVDEGFDVR GGLLESGTVG RWLAKHADDG RRSGLAIVGT HLPHGGDATA
     LAVAAADGNG GYIDTAMLTP DDDDALAAWL ADPDNPKALH EAKLAMHDLA GRGWTLGGIT
     SDTALAAYLV RPGQRSFTLD DLSLRYLRRE LRAETPEQEQ FSLLDNVDEV DKQAIQTLIL
     RARAVVDLAA ALDAELDLID STSLLGEMEL PVQQVLADME KAGIAADLRL LTELQSQFGD
     QIRDAAEAAY AVIGKQINLS SPKQLQVVLF EELGMPKTKR TKTGYTTDAD ALQSLFCKTE
     HPFLQHLLTH RDVTRLKVTV DGLLNAVAAD GRIHTTFNQT IATTGRLSST EPNLQNIPIR
     TNAGRQIRDA FVVGSENNGY TELMTADYSQ IEMRIMAHLS RDEGLIEAFH TGEDLHSFVA
     SRAFGIPIED ITPELRRRVK AMSYGLAYGL SAYGLATQLK ISTEEAKLQM EQYFARFGGV
     RDYLMDVVEQ ARKDGYTSTV LGRRRYLPEL DSSNRQIREA AERAALNAPI QGSAADIIKV
     AMIAVDKSLK QAKLASRMLL QVHDELLFEV AIGEREQIEA MVREQMGSAY PLDVPLEVSV
     GFGRSWGAAA H