DPO1_PSEAE
ID DPO1_PSEAE Reviewed; 913 AA.
AC Q9HT80;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=DNA polymerase I;
DE Short=POL I;
DE EC=2.7.7.7;
GN Name=polA; OrderedLocusNames=PA5493;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 3'-5' and 5'-3' exonuclease activity. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- SUBUNIT: Single-chain monomer with multiple functions. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
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DR EMBL; AE004091; AAG08878.1; -; Genomic_DNA.
DR PIR; F82958; F82958.
DR RefSeq; NP_254180.1; NC_002516.2.
DR RefSeq; WP_003114123.1; NZ_QZGE01000012.1.
DR AlphaFoldDB; Q9HT80; -.
DR SMR; Q9HT80; -.
DR STRING; 287.DR97_2871; -.
DR PaxDb; Q9HT80; -.
DR PRIDE; Q9HT80; -.
DR EnsemblBacteria; AAG08878; AAG08878; PA5493.
DR GeneID; 877794; -.
DR KEGG; pae:PA5493; -.
DR PATRIC; fig|208964.12.peg.5758; -.
DR PseudoCAP; PA5493; -.
DR HOGENOM; CLU_004675_0_0_6; -.
DR InParanoid; Q9HT80; -.
DR OMA; NRPPMPD; -.
DR PhylomeDB; Q9HT80; -.
DR BioCyc; PAER208964:G1FZ6-5620-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; ISS:PseudoCAP.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IBA:GO_Central.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR CDD; cd09898; H3TH_53EXO; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10133; PTHR10133; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; SSF47807; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
DR TIGRFAMs; TIGR00593; pola; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; DNA replication; DNA-binding;
KW DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease;
KW Nucleotidyltransferase; Reference proteome; Transferase.
FT CHAIN 1..913
FT /note="DNA polymerase I"
FT /id="PRO_0000287729"
FT DOMAIN 1..305
FT /note="5'-3' exonuclease"
FT DOMAIN 306..501
FT /note="3'-5' exonuclease"
FT REGION 505..913
FT /note="Polymerase"
SQ SEQUENCE 913 AA; 99793 MW; 6BF73DBBD41F62D7 CRC64;
MSQAPLVLVD GSSYLYRAFH ALPPLTTSTG KPTGAVKGVL NMLLSLRKQY PDSPFAVVFD
AKGPTFRDEL FAEYKANRPS MPDDLRVQVE PLHASVRALG LPLLCVEGVE ADDVIGTLAR
SSAAADRPVV ISTGDKDMAQ LVDGHITLVN TMTGSRLDVD GVKEKFGVGP ELIIDFLALM
GDKVDNIPGV PGVGEKTALG LLTGVGGGLE VLYASLDKVP ELPIRGAKGL PAKLEENREQ
AFLSYQLATI KTDVELDVEI DKLYPGEPQR EALIALYREL EFKNWLDDLL REAKEAGENG
EAETPIQAEV DYDVVLDQAG FDAWLKKLEE AELIAFDTET TSIDAQQAQV VGVSFAVKEG
EAAYVPLAHS YMGVPEQLDR DAVLRALKPL LEDPKKLKVG QHAKYDINVL ANASTPIALR
GVAYDTMLES YVLDSTATRH DMDSLALKYL GHSTIRFEDI AGKGAKQLTF DQIAIEQAGP
YAAEDADVTL RLHQALWQKL EAIPSLARVL TDIEMPLVPV LARIERNGAL VDANLLGIQS
RELGEKMVAL ERQAYDLAGQ EFNLGSPKQL GAILYDKLGL PVLSKTATGQ PSTAEAVLAE
LAEQDFELPK VIMQYRSMSK LKSTYTDRLP EQINPRTGRI HTSYHQAVAA TGRLSSSDPN
LQNIPIRTAE GRRIRQAFVA PQGYKLLAAD YSQIELRIMA HLAKDDGLLD AFRHDLDVHR
ATAAEVFGVP LEDVSGDQRR SAKAINFGLI YGMSAFGLAK QIGVERKEAQ AYIDRYFARY
PGVLAYMERT RAQAAEQGFV ETLFGRRLYL PEIHSKNGAM RKAAERTAIN APMQGTAADI
MKRAMVAVDN WLQESGLDAR VILQVHDELV LEVREDLVEQ VCEGIRPLMS GAATLDVPLV
VEAGVGSNWD EAH
