ID   DPO1_RICTY              Reviewed;         872 AA.
AC   Q9RLA0;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=DNA polymerase I;
DE            Short=POL I;
DE            EC=2.7.7.7;
GN   Name=polA; OrderedLocusNames=RT0763;
OS   Rickettsia typhi (strain ATCC VR-144 / Wilmington).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=257363;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC VR-144 / Wilmington;
RX   PubMed=10486973; DOI=10.1093/oxfordjournals.molbev.a026208;
RA   Andersson J.O., Andersson S.G.E.;
RT   "Genome degradation is an ongoing process in Rickettsia.";
RL   Mol. Biol. Evol. 16:1178-1191(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-144 / Wilmington;
RX   PubMed=15317790; DOI=10.1128/jb.186.17.5842-5855.2004;
RA   McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E.,
RA   McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E.,
RA   Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A., Hong C.,
RA   Yu X.-J., Walker D.H., Weinstock G.M.;
RT   "Complete genome sequence of Rickettsia typhi and comparison with sequences
RT   of other Rickettsiae.";
RL   J. Bacteriol. 186:5842-5855(2004).
CC   -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC       exhibits 5'-3' exonuclease activity. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: Single-chain monomer with multiple functions. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ238757; CAB56108.1; -; Genomic_DNA.
DR   EMBL; AE017197; AAU04219.1; -; Genomic_DNA.
DR   RefSeq; WP_011191194.1; NC_006142.1.
DR   AlphaFoldDB; Q9RLA0; -.
DR   SMR; Q9RLA0; -.
DR   STRING; 257363.RT0763; -.
DR   EnsemblBacteria; AAU04219; AAU04219; RT0763.
DR   KEGG; rty:RT0763; -.
DR   eggNOG; COG0258; Bacteria.
DR   eggNOG; COG0749; Bacteria.
DR   HOGENOM; CLU_004675_0_0_5; -.
DR   OMA; NRPPMPD; -.
DR   OrthoDB; 1220182at2; -.
DR   Proteomes; UP000000604; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR   CDD; cd09898; H3TH_53EXO; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR   InterPro; IPR002421; 5-3_exonuclease.
DR   InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR   InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR020045; DNA_polI_H3TH.
DR   InterPro; IPR018320; DNA_polymerase_1.
DR   InterPro; IPR002298; DNA_polymerase_A.
DR   InterPro; IPR008918; HhH2.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR10133; PTHR10133; 1.
DR   Pfam; PF01367; 5_3_exonuc; 1.
DR   Pfam; PF02739; 5_3_exonuc_N; 1.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   PRINTS; PR00868; DNAPOLI.
DR   SMART; SM00475; 53EXOc; 1.
DR   SMART; SM00279; HhH2; 1.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF47807; SSF47807; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00593; pola; 1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; DNA-binding;
KW   DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease;
KW   Nucleotidyltransferase; Transferase.
FT   CHAIN           1..872
FT                   /note="DNA polymerase I"
FT                   /id="PRO_0000280951"
FT   DOMAIN          1..283
FT                   /note="5'-3' exonuclease"
SQ   SEQUENCE   872 AA;  99310 MW;  BC2EBE3490879A08 CRC64;
     MTKKNTLLLI DGYGFVFRAY YAQQSLSSAK GEPVGALYGF TSMLLKLLSD FKPQYGAIVF
     DSGGKNFRHK IYQNYKANRP TPPEDLIGQL PLIRDVASHL NFAILEKNGY EADDIIATFA
     TKTITLGKEV IIISSDKDLL QLMSKNIKIY DPIKCKYITE DDVIIKFGTT PDKLREVMAL
     IGDRSDNIPG VPSIGPKTAS SLITQFGSVE NIFNSLDQIS SIKQRKTLQN SREAALISWR
     LIGLDSNVDL DFNLNNLEWS HPNSEKLIGF LQKYGFKSLY KRVENLFYIK INDHEEIVDN
     KVTEAKEISN ASELENFAKE AEKIGIFGIY LLQQKGNNCA LILSLQNQSY IIKITNNNLF
     PYNDNIKNNN DWFSYIILNL LTNKSIKKIT YSLKHLLKFY ANQSHKITAI EDLELMQYTL
     SAGLVQKNLF AETLTKDNII NESAKIVINF ISLYKQTLLA LQKNKAFRLY REIDLPTCFI
     LDKMEKIGIK VDANYLHQLS DEFGTEILKI EEEIFALSGT KFNIASQKQL SEILFKKMQL
     PSGNTLAKTS SYSTKAGILK KLSEDGYHIA TLLLRWRQLT KLKNTYTDSL PKQINNITKR
     IHTTFLQTST TTGRLSSQEP NLQNIPTRSS DGNKIRQAFI AEDGYKLISA DYSQIELRIL
     SHIANVDVLK QAFINKEDIH TQTACQIFNL QKHELTSEHR RKAKAINFGI IYGISAFGLA
     KQLNVSNGTA AEYIKQYFAE YKGMQEYMVQ TKAYANRNGY VTNFFGRKCF IPLIHDKKLK
     QFAERAAINA PIQGTSADII KIAMIKLAQE IEKRKLKTRL ILQIHDELLF EVPEIELELV
     IPIIKKIMEY STNIDVPIIT EIRAGNNWKE IH