ID   DPO2_KLULA              Reviewed;         994 AA.
AC   P05468;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=DNA polymerase;
DE            EC=2.7.7.7;
DE   AltName: Full=Plasmid PGKL-2 protein 2;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OG   Plasmid pGKl-2.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=3041369; DOI=10.1093/nar/16.13.5863;
RA   Tommasino M., Ricci S., Galeotti C.L.;
RT   "Genome organization of the killer plasmid pGKL2 from Kluyveromyces
RT   lactis.";
RL   Nucleic Acids Res. 16:5863-5878(1988).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- MISCELLANEOUS: This DNA polymerase requires a protein as a primer.
CC   -!- MISCELLANEOUS: The presence of the two linear plasmids, termed pGKl-1
CC       and pGKl-2, in strains of Kluyveromyces lactis confers the killer
CC       phenotype, i.e. production of toxin and resistance to it, to the host
CC       cell.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; X07776; CAA30603.1; -; Genomic_DNA.
DR   PIR; S00960; S00960.
DR   AlphaFoldDB; P05468; -.
DR   PRIDE; P05468; -.
DR   Proteomes; UP000000598; Plasmid pGKL2.
DR   GO; GO:0110165; C:cellular anatomical entity; IEA:UniProt.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR004868; DNA-dir_DNA_pol_B_mt/vir.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF03175; DNA_pol_B_2; 1.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Plasmid; Reference proteome; Transferase.
FT   CHAIN           1..994
FT                   /note="DNA polymerase"
FT                   /id="PRO_0000046549"
SQ   SEQUENCE   994 AA;  117561 MW;  1278F3CC2D07E1E5 CRC64;
     MNDELAFLNS QIDDYSDDIE FLRTEVFRIN RYSKSIYSLM KNSSKEAIKE TIEFNKYGQE
     IHSKIKEKEI SLIEAIKLME KKYKYLRSLN KTKDKSKKKK IHTKDIETLK TIYKTKADFD
     IRNFKNAETF TYENYSLLIP SEKELIDLIP EIEEDYSYYF YVSLTITGPT IFGGVTRKVP
     IIDTYGDINK DFYNKIKENI TKVLDRVPFY EEKESEIICK KVLITLTVNK WESIDKWYKN
     VKSKSKKFKD FTLFLASDTS KNCVQQCVEY LGGKWNTNYS LEKMIPQQKI VTYIPLLMDI
     QYVQSMEDLI SDFDEPNTDC KNIARLLKWN GHMGVITKID KDVKINRIQR KRIIDVENKE
     VKEVFFDIES FSDETKHQIP YLICWSYLGE LYYRTGKNCI KEFVEEILSY NVDIILYAWY
     GSGYDYQHVL PYMKSKCIKD KYIIKNNMIT YGELYYENAI IYLKDPYLFI LTSLDKASKA
     FNVINKGEFP HSIIHSWEDL NKILPNWVKI QKRMIEYKDD NKLNIYYKNI EIFENDRNYN
     TILENAIEYC KVDVLAMEKV WIKFKKLLEK NLNITVSVKT FTLSQLSMKI MESKLPKYVK
     LYVPTLEEYS KIRNAIYGGR VISKNGIYEE NIVYADVVSL YPSAMKLLKH SYGKPKLVDY
     IDFEKHGIYR VTLLNKHDQE PKNYLNFVPR RINRKLTWEW FKIHEGWYHT YDLIIAKSEG
     FDIYCHEGFE YPEKDYIFND FIDTLYSMKE THTACSCEEQ PCPIRMVAKI ALNGGGYGKF
     VQKPIDKEIY IVKRDVVAGE CDKLQENENG EICIGKRLVK RPLFYNLDSE NYDKMIIEKE
     DEPIYSTQCG ISILSASRYR LYKLCKQFEN IDIIYSDTDS IFVKQKSVDW ELFKSKCGSE
     LGQLDSTIDD TKNAIISKMY IGGAKMYAFE YLNNKNKLIT KLHCKGVPNY MLSLNQFKYL
     MESPDNTIAY KFEIIRRKLV NVITKDLIKD IKQT