ID   DPO3A_BUCAI             Reviewed;        1161 AA.
AC   P57332;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=DNA polymerase III subunit alpha;
DE            EC=2.7.7.7;
GN   Name=dnaE; OrderedLocusNames=BU238;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC       responsible for most of the replicative synthesis in bacteria. This DNA
CC       polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC       is the DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC       and theta chains) that associates with a tau subunit. This core
CC       dimerizes to form the PolIII' complex. PolIII' associates with the
CC       gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC       and with the beta chain to form the complete DNA polymerase III complex
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BA000003; BAB12953.1; -; Genomic_DNA.
DR   RefSeq; NP_240067.1; NC_002528.1.
DR   RefSeq; WP_010896021.1; NC_002528.1.
DR   AlphaFoldDB; P57332; -.
DR   SMR; P57332; -.
DR   STRING; 107806.10038918; -.
DR   PRIDE; P57332; -.
DR   EnsemblBacteria; BAB12953; BAB12953; BAB12953.
DR   KEGG; buc:BU238; -.
DR   PATRIC; fig|107806.10.peg.251; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_0_0_6; -.
DR   OMA; NECRRMG; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..1161
FT                   /note="DNA polymerase III subunit alpha"
FT                   /id="PRO_0000103312"
SQ   SEQUENCE   1161 AA;  132796 MW;  9C62824CCE73A9EB CRC64;
     MNEPKFIHLH VHSDYSMIDG LSKPEDLVKK AASLNMPAIA ITDYNNLYGV IKFYNMAHKL
     GLKPIIGITV NFFSDLINNQ LTKLTLLAST QEGYKNLILL ISRAYQKGYV HNNHVTIEKK
     WFSEINKGLI LLSGGPQGEL GKVLLNGQSS LISICLSFYQ KYFPDSYYLE LFRTNRDNEE
     TYLHLAVDLS LSTGVPIVAT NDVCFLSQED FKIHKIRIAI NEGEILQHSK IQNNYSNQQF
     LKTTEEMSDL FSDIPEALIN SVEIAKRCNV FIHSGKYFLP QFRTGKISVE KYLIKEAYKG
     IKKRLNSRYL DIEKYKDIYS QYINRLDMEL TVINKMGFPG YFLIVMEFIQ WAKDNNIPVG
     PGRGSGAGSL VAYALNITEV DPLSFNLLFE RFLNPERISM PDFDIDFCME KRDQVIDHVS
     EIYGKNAVAQ IITFGTLTAK AVIRDVGRVL GYPYGFINKL SKLVPLDPGI TLKEAFSKES
     ELFNLYKNNE DVKNLIDIAK KLEGTNRNIG KHAGGVVISP TKITDFCPLY CDEKGNNPVT
     QFDKNDIEYI GLVKFDFLGL RTLTIISYAV EMINTKLNLN KKRTININSI PLDDAKCFKL
     LKNSETTGVF QLESYGMKDL IKRLQPDCFE DMIALVALFR PGPLQSGMVD NFINRKHGRE
     NISYPDHKWQ HILLKPILES TYGIILYQEQ VMEIAQVLSG YTLGSADILR RAMSKKNLKD
     MSEQRAIFEE GASNNGIDKK LSIKIFDLLE KFAGYGFNKS HSVAYALVSY QTLWLKSHYP
     AEFIAAAMTS DIDNTEKIVI LINESLNMGV KIIPPNINLS KYEFYVNDQG DIVYGLGAIK
     GIGKNSVQVL IKEREENKFF HDLFDLCMRI DSQKITRRVL EKLIMSGSCD CFDKNRNYLL
     QSIDDAINAS KESFRIKSFK QDSLFGIFQN ELNQVKKNNL VNLVCPEKNK LQNEYQVLGF
     YLTGHPINQY KKELECYVNG VKLSQLKFIK RNKKILVAGI IVSIKIKITK NKNRIAILVL
     DDNTSRLEVV IFTELLNAYE SLVKLNKLLF VHGMLNVSFI DKNIKMTAYA LIDLSVLRKK
     YINKLIIILN KEKTNIFFLN NLYECLDKQS KGNIPVFIFF DNKEVCLNKK LNKKWFVVIT
     DDFLNKLKFL VGSKNIELKF N