ID   DPO3A_HAEIN             Reviewed;        1159 AA.
AC   P43743;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=DNA polymerase III subunit alpha;
DE            EC=2.7.7.7;
GN   Name=dnaE; OrderedLocusNames=HI_0739;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC       responsible for most of the replicative synthesis in bacteria. This DNA
CC       polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC       is the DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC       and theta chains) that associates with a tau subunit. This core
CC       dimerizes to form the PolIII' complex. PolIII' associates with the
CC       gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC       and with the beta chain to form the complete DNA polymerase III complex
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L42023; AAC22399.1; -; Genomic_DNA.
DR   PIR; H64089; H64089.
DR   RefSeq; NP_438899.1; NC_000907.1.
DR   RefSeq; WP_005693144.1; NC_000907.1.
DR   AlphaFoldDB; P43743; -.
DR   SMR; P43743; -.
DR   STRING; 71421.HI_0739; -.
DR   EnsemblBacteria; AAC22399; AAC22399; HI_0739.
DR   KEGG; hin:HI_0739; -.
DR   PATRIC; fig|71421.8.peg.776; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_0_0_6; -.
DR   OMA; NECRRMG; -.
DR   PhylomeDB; P43743; -.
DR   BioCyc; HINF71421:G1GJ1-778-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..1159
FT                   /note="DNA polymerase III subunit alpha"
FT                   /id="PRO_0000103322"
SQ   SEQUENCE   1159 AA;  129789 MW;  21F09BE45A3DCE48 CRC64;
     MSSQPRFIHL RTHTDFSMID SIVKVKPLVK ACAANEMVAM GLTDFTNFCG VVSFYSEMLS
     SGMKPIIGAD VKVKSPLCGD EYFDLTLLAK NNEGYRNITL LLSKAYQRGY NDLPYIDQDW
     LIEHRDGVII LSGGTQGDVG KKLLKENVAE IESAVAFYQE FFADHFYLAL SRTGRPNEER
     YIQAALKLAE RCDLPLVATN DVMFLNTEDF EAHEIRVAIH DGYTLDDPKR PKCYTSQQYF
     RSEEDMCKLF ADIPSALENT LLIAQRCSVT LRLGQYFLPQ FPTGDLSTED FLVQKSKEGL
     EERLVFLFPD EKVRLEKRPK YDERLQVELD VINQMGFPGY FLIVMEFIQW SKDNDIPVGP
     GRGSGAGSLV AYALKITDLD PLEFDLLFER FLNPERVSMP DFDVDFCMDG RDRVIEHVAE
     TYGRGAVSQI ITFGTMAAKA VIRDVGRVLG HPYGFVDRIS KLIPPDPGMT LAKAFETEPQ
     LQTAYDSDEE VRALINMARK LEGVTRNAGK HAGGVVISPT LITDFAPLYC DNEGLHPVTH
     FDKNDVEYAG LVKFDFLGLR TLTIIKWALD IINVRMVREG KPRVDIAAIP LDDPESFELL
     KRSETTAVFQ LESRGMKDLI KRLQPDCFED IIALVALFRP GPLQSGMVDN FIDRKHGREE
     VSYPDAEYQH ASLKPILEPT YGIILYQEQV MQIAQVLAGY TLGGADLLRR AMGKKKPEEM
     AKQRLVFKEG AEKNGIDGEL SMKIFDLVEK FAGYGFNKSH SAAYALVSYQ TLWLKTHFPA
     EFMAAVMTSE MDNTDKIVGL YDECLRMGLK VTPPDINIGK HHFSVNEQGE IVYGIGAIKG
     VGEGPIEALV AARNEGGIFK DLFDLCARVD LKKINRRTFE SLIMSGAFDK LGPHRAALSK
     NLEDALRASD QHAKDEAMGQ TDMFGVLTET HEDVENAYAN TPPYTEKQIL DGERETLGLY
     LSSHPVSRYL KELSHYTSTR LKDLAPNRRG QISTVAGLVV ASRIAMTKKG NRLGIATLDD
     RSGRLDLTLF GESLEQFGEK LQKDTVIVAS GQVSFDDFSG GLKMTVRELM TLDEARSRYV
     KSLAISLSEH QITPSFIKQF KALLEPVSGG TLPINVYYQS PKGRALLRLG VQWSIIPTDE
     ILTELVNLLG ESAVELEFE