ID   DPO3A_HELPY             Reviewed;        1211 AA.
AC   P56157;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 120.
DE   RecName: Full=DNA polymerase III subunit alpha;
DE            EC=2.7.7.7;
GN   Name=dnaE; OrderedLocusNames=HP_1460;
OS   Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA   Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA   Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA   McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA   Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA   Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA   Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
CC   -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC       responsible for most of the replicative synthesis in bacteria. This DNA
CC       polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC       is the DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC       and theta chains) that associates with a tau subunit. This core
CC       dimerizes to form the PolIII' complex. PolIII' associates with the
CC       gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC       and with the beta chain to form the complete DNA polymerase III complex
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE000511; AAD08502.1; -; Genomic_DNA.
DR   PIR; D64702; D64702.
DR   RefSeq; NP_208251.1; NC_000915.1.
DR   RefSeq; WP_000662171.1; NC_018939.1.
DR   AlphaFoldDB; P56157; -.
DR   SMR; P56157; -.
DR   DIP; DIP-3526N; -.
DR   IntAct; P56157; 3.
DR   MINT; P56157; -.
DR   STRING; 85962.C694_07560; -.
DR   PaxDb; P56157; -.
DR   PRIDE; P56157; -.
DR   EnsemblBacteria; AAD08502; AAD08502; HP_1460.
DR   KEGG; hpy:HP_1460; -.
DR   PATRIC; fig|85962.47.peg.1571; -.
DR   eggNOG; COG0587; Bacteria.
DR   OMA; KQRFHLC; -.
DR   PhylomeDB; P56157; -.
DR   Proteomes; UP000000429; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..1211
FT                   /note="DNA polymerase III subunit alpha"
FT                   /id="PRO_0000103323"
SQ   SEQUENCE   1211 AA;  138053 MW;  FB248DD47E4CBB0C CRC64;
     MKENKAFTHL HLHTEYSLLD GANKIKILAK RVKELGMKSV SVTDHGNMFG AIDFYTSMKK
     EGIKPIIGME AYIHNDDNLS SKETKQRFHL CLFAKNQEGY ENLMFLSSMA YLEGFYYFPR
     INKKLLKEHS KGIIASSACL QGEVNYHLNT NNERNRKYGA KGYDEAKKIA CEYQEIFEDD
     FYLEIMRHGI LDQRFIDEQV IKMSLETGLK IIATNDTHYT MPNDAKAQEV AMCVAMGKTL
     NDKGRLKHSV HEFYIKSPEE MAKLFADIPE ALENTQEIAD KCVLEIDLKD DKKNPPTPPS
     FKFTKAYAQN EGLNFEDDAS YFAYKAREGL KERLVLVPKE KHDQYKERLE KEIEVITNMK
     FPGYMLIVWD FIRYAKEMGI PVGPGRGSAA GSLVAFALKI TDIDPLKYDL LFERFLNPER
     ISMPDIDTDF CQRRRKEIIE YMIEKYGKYN VAQVITFNKM LAKGVIRDVA RVLDMPYKEA
     DDFAKLIPNR LGITLKGYEK NGEFIEGAWE LEPKIKELVE SNELAKQVWE YSLNLENLNR
     NAGVHAAALV VDSQKELWHK TPLFASEKTG GIVTQYSMKY LEPVDLIKFD FLGLKTLTVI
     DDALKIIKTQ HKISVDFLSL DMDDPKVYKT IQSGDTVGIF QIESGMFQGL NKRLRPSSFE
     DIIAIIALGR PGPMESGMVD DFVNRKHGVE PIAYAFKELE PILKPTYGTI VYQEQVMQIV
     QTIGGFSLGE ADLIRRAMGK KDAQIMADNK AKFVEGAKNL GHDGQKAANL WDLIVKFAGY
     GFNKSHSAAY AMITFQTAYL KTYYKHEFMA AMLTSESNKI ESVARYIDEV RALEIEVMPP
     HINSSMQDFS VAEFKNQKGE LEKKIVFGLG AIKGVGGEPI KNIIEERAKG DYKSLEDFIS
     RVDFSKLTKK SLEPLVKSGS LDNLGYTRKT MLANLDLICD AGRAKDKANE MMQGGNSLFG
     AMEGGTKEQV VLDMIDLGEH DAKTLLECEY ETLGIHVSGN PLDEFKEEIK GFKNLVKSID
     IEELEIGSQA YLLGKIMEVK KKIGKRSGKP YGIADILDRY GKFELMLFEK QLNALEELDI
     NKPLVFKCKI EEQEEVVRLR LFEILDLESA REVKIPKARY KDPEKQKEEV REIPPMEMLA
     SSSCSLAIVL ENDVKKEFLR QIKESALKHQ GKRPLYLIIK DKDKQFKIQS DLMVNEKIKD
     DFKGLEWRDL A