ID   DPO3A_PSEAE             Reviewed;        1173 AA.
AC   Q9HXZ1; Q9F662;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=DNA polymerase III subunit alpha;
DE            EC=2.7.7.7;
GN   Name=dnaE; OrderedLocusNames=PA3640;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Oliver A., Baquero F., Blazquez J.;
RT   "Naturally-ocurring dnaE Pseudomonas aeruginosa mutations.";
RL   Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC       responsible for most of the replicative synthesis in bacteria. This DNA
CC       polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC       is the DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC       and theta chains) that associates with a tau subunit. This core
CC       dimerizes to form the PolIII' complex. PolIII' associates with the
CC       gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC       and with the beta chain to form the complete DNA polymerase III complex
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE004091; AAG07028.1; -; Genomic_DNA.
DR   EMBL; AF297647; AAG16710.2; -; Genomic_DNA.
DR   PIR; H83189; H83189.
DR   RefSeq; NP_252330.1; NC_002516.2.
DR   RefSeq; WP_003098578.1; NZ_QZGE01000001.1.
DR   AlphaFoldDB; Q9HXZ1; -.
DR   SMR; Q9HXZ1; -.
DR   STRING; 287.DR97_4299; -.
DR   PaxDb; Q9HXZ1; -.
DR   PRIDE; Q9HXZ1; -.
DR   EnsemblBacteria; AAG07028; AAG07028; PA3640.
DR   GeneID; 880490; -.
DR   KEGG; pae:PA3640; -.
DR   PATRIC; fig|208964.12.peg.3809; -.
DR   PseudoCAP; PA3640; -.
DR   HOGENOM; CLU_001600_0_0_6; -.
DR   InParanoid; Q9HXZ1; -.
DR   OMA; NECRRMG; -.
DR   PhylomeDB; Q9HXZ1; -.
DR   BioCyc; PAER208964:G1FZ6-3710-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..1173
FT                   /note="DNA polymerase III subunit alpha"
FT                   /id="PRO_0000103334"
SQ   SEQUENCE   1173 AA;  130905 MW;  AE3DFE97D82EB3B0 CRC64;
     MTVSFVHLRL HTEFSLVDGL VRVKPLAKAV AGLGMPAVAV TDQSNMCSLV KFYKTAMGAG
     IKPICGADIW LASREEDGPL SRLSLLAMNA KGYRNLTELI SRGWSEGQRN GEIIIERDWV
     KEAAEGLIAL SAAKEGEIGH ALLDGEEAKA EALLREWMEV FPERFYVEVQ RTSRVNDEEH
     LHAAVALASR CNAPLVATND VRFIKQEDFE AHETRVCIGE GRTLDDPRRP RTYSDQQYLK
     SPAEMAELFS DLPEALENTV EIAKRCNIEV QLGKYFLPDF PTPNGMGIDD YLRHASFEGL
     EERLEVLLPK DTPDYEAKRQ VYVDRLNFEL DIIIQMGFPG YFLIVMDFIK WAKNNGVPVG
     PGRGSGAGSL VAYVLKITDL DPLAYDLLFE RFLNPERISM PDFDVDFCME GRDRVIDYVA
     DAYGRNAVSQ IITFGTMAAK AVVRDVARVQ GKSYGLADRL SKMIPFEVGM TLDKAYEQEE
     MLRDFLKSDE EAAEIWEMAL KLEGITRGTG KHAGGVVIAP TKLTDFSPIA CDEEGGGLVT
     QFDKDDVEAA GLVKFDFLGL RTLTIIKWAM EIINREQAKK GLEPVNIDFI PLDDKPTYSL
     LQKAETTAVF QLESRGMKEL IKKLKPDCLE DLIALVALFR PGPLQSGMVD DFINRKHGRA
     ELSYPHPDYQ YAGLEPVLKP TYGIILYQEQ VMQIAQVMAG YTLGGADMLR RAMGKKKPEE
     MAKQRGGFIE GCKNNGIDAD LAGNIFDLVE KFAGYGFNKS HSAAYGLVSY QTAWLKTHFP
     APFMAAVLTA DMQNTDKVVT LIEECRHMKL RIVAPDVNNS EFRFTVDDDG RIVYGLGAIK
     GVGEGPVEAI TECRAEGGPF NTLFDFCDRV DLKRINKRTL EALIRAGALD RLGPHYHDEL
     KAYQATVDLN RAVLLAAMEE AIQAAEQTAR SHDSGHMDLF GGVFAEPEAD VYANHRKVKE
     LTLKERLKGE KDTLGLYLTG HPIDEYEGEV RRFARQRIVE LKPARDTQTV AGLIVNLRVM
     KNKKGDKMGF VTLDDRSGRI EASLFSEAFA AAQSLLQTDA LVVVEGEVSQ DDFSGGLRLR
     AKRVMSLEEA RTGLAESLRM KLHADLLKGD RLRWLGELFN RHRGSCPITL DYTSADAKAL
     LQFGESWRVD PADDLIQALR DQFGRDNVFL NYR