DPO3A_THEAQ
ID DPO3A_THEAQ Reviewed; 1220 AA.
AC Q9XDH5;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=DNA polymerase III subunit alpha;
DE EC=2.7.7.7;
GN Name=dnaE;
OS Thermus aquaticus.
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=271;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10229580; DOI=10.1007/pl00006520;
RA Huang Y.P., Ito J.;
RT "DNA polymerase C of the thermophilic bacterium Thermus aquaticus:
RT classification and phylogenetic analysis of the family C DNA polymerases.";
RL J. Mol. Evol. 48:756-769(1999).
CC -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC responsible for most of the replicative synthesis in bacteria. This DNA
CC polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC is the DNA polymerase (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC and theta chains) that associates with a tau subunit. This core
CC dimerizes to form the PolIII' complex. PolIII' associates with the
CC gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC and with the beta chain to form the complete DNA polymerase III complex
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC subfamily. {ECO:0000305}.
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DR EMBL; AF062920; AAD44403.1; -; Genomic_DNA.
DR PDB; 2HPI; X-ray; 3.00 A; A=1-1220.
DR PDB; 2HPM; X-ray; 3.70 A; A=1-1220.
DR PDB; 3E0D; X-ray; 4.60 A; A/B=1-1220.
DR PDB; 4IQJ; X-ray; 3.20 A; A/B/C/D=1-1220.
DR PDBsum; 2HPI; -.
DR PDBsum; 2HPM; -.
DR PDBsum; 3E0D; -.
DR PDBsum; 4IQJ; -.
DR AlphaFoldDB; Q9XDH5; -.
DR SMR; Q9XDH5; -.
DR BRENDA; 2.7.7.7; 6334.
DR EvolutionaryTrace; Q9XDH5; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.1600; -; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR004805; PolC_alpha.
DR PANTHER; PTHR32294; PTHR32294; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; SSF89550; 1.
DR TIGRFAMs; TIGR00594; polc; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA replication; DNA-directed DNA polymerase;
KW Nucleotidyltransferase; Transferase.
FT CHAIN 1..1220
FT /note="DNA polymerase III subunit alpha"
FT /id="PRO_0000103354"
FT STRAND 10..12
FT /evidence="ECO:0007829|PDB:4IQJ"
FT TURN 18..20
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 25..35
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 41..48
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 53..61
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 62..64
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 66..78
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 93..100
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 103..118
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 121..127
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 129..134
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 139..142
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 149..155
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 159..173
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 174..176
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 177..181
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 187..202
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 207..209
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 213..217
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 218..220
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 221..231
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 245..247
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 254..260
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 263..266
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 269..279
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 306..321
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 323..325
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 328..335
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 347..356
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 359..362
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 363..367
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 380..397
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 400..414
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 415..417
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 425..429
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 431..435
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 443..447
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 450..453
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 465..469
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 470..472
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 473..484
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 486..488
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 489..497
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 500..510
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 515..522
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 527..532
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 535..537
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 548..551
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 553..564
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 566..568
FT /evidence="ECO:0007829|PDB:4IQJ"
FT STRAND 569..581
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 586..588
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 592..594
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 600..604
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 606..610
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 611..613
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 614..622
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 623..639
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 645..647
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 653..660
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 673..682
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 687..696
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 701..703
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 704..712
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 724..726
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 727..734
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 735..737
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 744..754
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 759..771
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 774..776
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 777..791
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 796..808
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 809..811
FT /evidence="ECO:0007829|PDB:4IQJ"
FT HELIX 815..834
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 836..846
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 847..849
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 851..862
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 863..865
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 873..875
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 881..883
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 886..888
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 891..893
FT /evidence="ECO:0007829|PDB:4IQJ"
FT STRAND 894..896
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 899..911
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 918..924
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 927..929
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 932..941
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 942..944
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 945..947
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 950..971
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 977..980
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 995..1006
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 1014..1017
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 1020..1024
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 1029..1031
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 1032..1036
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1037..1042
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1044..1051
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1068..1072
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1075..1079
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1098..1105
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1113..1120
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 1121..1124
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1129..1135
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 1141..1143
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 1147..1153
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1155..1166
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1168..1181
FT /evidence="ECO:0007829|PDB:2HPI"
FT HELIX 1185..1189
FT /evidence="ECO:0007829|PDB:2HPI"
FT TURN 1190..1193
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1195..1200
FT /evidence="ECO:0007829|PDB:2HPI"
FT STRAND 1208..1211
FT /evidence="ECO:0007829|PDB:4IQJ"
FT HELIX 1214..1217
FT /evidence="ECO:0007829|PDB:4IQJ"
SQ SEQUENCE 1220 AA; 137390 MW; 64EC8B0CE1F44A2B CRC64;
MGSKLKFAHL HQHTQFSLLD GAAKLQDLLK WVKETTPEDP ALAMTDHGNL FGAVEFYKKA
TAMGVKPIIG YEAYVAAESR FDRKRGKGLD GGYFHLTLLA KDFTGYQNLV RLASRAYLEG
FYEKPRIDRE ILREHAQGLI ALSGCLGAEI PQFILQDRLD LAEARLNEDL SIFGDRFFIE
IQNHGLPEQK KVNQVLKEFA RKYGLGMVAT NDGHYVRKED ARAHEVLLAI QSKTTLDDPE
RWRFPCDEFY VKTPEEMRAM LPEAEWGDEP FDNTVEIARM CDVDLPIGDK MVYRIPRFPL
PEGRTEAQYL RELTFLGLLR RYPDRITEAF YREVLRLLGT MPPHGDERAL AEALARVEEK
AWEELRKRLP PLEGVREWTA EAILHRALYE LSVIERMGFP GYFLIVQDYI NWARGHGVSV
GPGRGSAAGS LVAYAVGITN IDPLRFGLLF ERFLNPERVS MPDIDTDFSD RERDRVIQYV
RERYGEDKVA QIGTFGSLAS KAALKDVARV YGIPHKKAEE LAKLIPVQFG KPKPLQEAIQ
VVPELRAEME KDERIRQVIE VAMRLEGLNR HASVHAAGVV IAAEPLTDLV PLMRDQEGRP
VTQYDMGAVE ALGLLKMDFL GLRTLTFLDE ARRIVKESKG VELDYDRLPL DDPKTFELLS
RGETKGVFQL ESGGMTATVR GLKPRRLEDI IALVSLYRPG PMEHIPTYIR RHHGQEPVSY
AEFPHAEKYL RPILDETYGI PVYQEQIMQI ASQVAGYSLG EADLLRRAMG KKRVEEMQKH
RERFVRGAKE RGVPEEEANR LFDMLEAFAN YGFNKSHAAA YSLLSYQTAY VKAHYPVEFM
AALLSVERHD SDKVAEYIRD ARALGIPVLP PDVNRSGFDF KVVGEEILFG LSAVKNVGEM
AARAILEERE RGGPFKSLGD FLKRLPEQVV NKRALESLVK AGALDAFGDR ARLLASLEPL
LRWAAETRER GRSGLVGLFA EVEEPPLVEA SPLDEITMLR YEKEALGIYV SGHPVLRYPG
LREVASCTIE ELSEFVRELP GKPKVLLSGM VEEVVRKPTR SGGMMARFTL SDETGALEVV
VFGRAYEGVS PKLKEDIPLL VLAEVEKGEE LRVLAQAVWT LEEVLEAPKA LEVEVDHALL
DEKGVARLKS LLDEHPGSLP VYLRVLGPFG EALFALREVR VGEEALGLLE AEGYRAYLVP
DREVFLQGNG GGPKEEVVPF
