ID   DPO3A_TREPA             Reviewed;        1170 AA.
AC   O83675;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 127.
DE   RecName: Full=DNA polymerase III subunit alpha;
DE            EC=2.7.7.7;
GN   Name=dnaE; OrderedLocusNames=TP_0669;
OS   Treponema pallidum (strain Nichols).
OC   Bacteria; Spirochaetes; Spirochaetales; Treponemataceae; Treponema.
OX   NCBI_TaxID=243276;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nichols;
RX   PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA   Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA   Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA   Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M., Utterback T.R.,
RA   McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A.,
RA   Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O.,
RA   Venter J.C.;
RT   "Complete genome sequence of Treponema pallidum, the syphilis spirochete.";
RL   Science 281:375-388(1998).
CC   -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC       responsible for most of the replicative synthesis in bacteria. This DNA
CC       polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC       is the DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC       and theta chains) that associates with a tau subunit. This core
CC       dimerizes to form the PolIII' complex. PolIII' associates with the
CC       gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC       and with the beta chain to form the complete DNA polymerase III complex
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE000520; AAC26567.1; -; Genomic_DNA.
DR   PIR; H71295; H71295.
DR   RefSeq; WP_010882114.1; NC_021490.2.
DR   AlphaFoldDB; O83675; -.
DR   SMR; O83675; -.
DR   IntAct; O83675; 2.
DR   STRING; 243276.TPANIC_0669; -.
DR   PRIDE; O83675; -.
DR   EnsemblBacteria; AAC26567; AAC26567; TP_0669.
DR   GeneID; 57879193; -.
DR   KEGG; tpa:TP_0669; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_0_0_12; -.
DR   OMA; NECRRMG; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000000811; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..1170
FT                   /note="DNA polymerase III subunit alpha"
FT                   /id="PRO_0000103356"
SQ   SEQUENCE   1170 AA;  132313 MW;  431FC4629FDE09F1 CRC64;
     MARMSFVHLH VHSNYSLLDG ASSLQRLVRT AKSLGQEALA LTDHGNMFGA LHFQKVCSAE
     GIKAIIGCEL YVAPESRFDR SEHTIGRRYY HLIVLAKNET GYRNLMVLSS KAYIEGMYYK
     PRVDDELLAQ HAEGLICLSS CLAGQLPYLL LQGRKREAEE HARKYRALFG VDNYFIEVQD
     HGLDEEKKVA PLLIELACRL GIPLVVTNDV HYAEQEDSVA QDILLCIGTK KNRSDPNRLK
     FKTDEFYLKS SEKMAQLFPH YPEMVLNTVR IAQRCNVRIP QPGPLLPLYQ IPHEFSSKEH
     YIRHLVHRGL YDRYAVVSEE IKARADYELD VIVRMDFVGY FLIVWDFITW AKEHDIPVGP
     GRGSGASSIV AYALKITDID PLRYKLLFER FMNPERISMP DFDIDFCFER RQEVIEYVRA
     RYGNDNVGQI ITFGTLKPKA AIRDVGRVLD IPLSEVLMIT KLMPDDPKLT FKKAYESEQL
     AQMKQEPRYA ELFQIAEKLE DTNRNTSLHA AGIVIGKTAL TDYVPLYKDS KTGKISTQFG
     MDLIEDCGLV KMDFLGLKTL TLIQRTQNLV RRKGGKYTTF SISDISDQDP TTFSMLAEGK
     SAAVFQFESR GMQGILKRAK PSKMEDLIAL NALYRPGPMA FIDQYIESKR DPGKIKYPDP
     CLEDILSETY GVIVYQEQVM QVAQRIAGFS LGEADILRRA MGKKKLAVMQ EKKKEFAERA
     EKQGFDKKHA ENIFEILIPF AGYGFNKSHA TAYSVVAYQT AFLKANFPAE FMAANLSNEI
     NSAEKLPLYM AEAEKMGLSI QKPDVNASEP YFSVCEGCIV YGLLGIKGLG EQVAFDVFDE
     RIRNGPYTSF VEVLDRVPAT SLNKKNAEIM IKAGCFDRFG VTRASLTAHL DDAMKYVARK
     KAVTSSRQAS LFDETDLGEC SEYTFPVMEE WSQRERLRIE KELMGYYISG HPLDEYRSVI
     GEKATLDLGH IENARSENKY LIVGVLNAIH PYTTKSGKNM AFGSFEDLHG SVDIVVFPVL
     WEEHRAQFLP ETIMGLVGTV DFSKETPAFL VDSVIDLEQL RFAQVKTILA GSEHRRVSSG
     EKTPLQKRGV SQEVHIEVSS HVRAHAQFKS LYEILSAHTG GSGEVFLHMH VDDRTYVVYV
     PSCKVSATEV FAQQLKGNES FVQILKECVQ