ID   DPO3A_XYLFA             Reviewed;        1193 AA.
AC   Q9PGU4;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 2.
DT   25-MAY-2022, entry version 120.
DE   RecName: Full=DNA polymerase III subunit alpha;
DE            EC=2.7.7.7;
GN   Name=dnaE; OrderedLocusNames=XF_0204;
OS   Xylella fastidiosa (strain 9a5c).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xylella.
OX   NCBI_TaxID=160492;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=9a5c;
RX   PubMed=10910347; DOI=10.1038/35018003;
RA   Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M.,
RA   Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S.,
RA   Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S.,
RA   Bueno M.R.P., Camargo A.A., Camargo L.E.A., Carraro D.M., Carrer H.,
RA   Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M.,
RA   Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H.,
RA   Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S.,
RA   Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H.,
RA   Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D.,
RA   Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E.,
RA   Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F.,
RA   Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N.,
RA   Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F.,
RA   Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y.,
RA   Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O.,
RA   Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A.,
RA   de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R.,
RA   Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B.,
RA   Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G.,
RA   Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M.,
RA   da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z.,
RA   Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D.,
RA   Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S.,
RA   Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C.;
RT   "The genome sequence of the plant pathogen Xylella fastidiosa.";
RL   Nature 406:151-159(2000).
CC   -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC       responsible for most of the replicative synthesis in bacteria. This DNA
CC       polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC       is the DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC       and theta chains) that associates with a tau subunit. This core
CC       dimerizes to form the PolIII' complex. PolIII' associates with the
CC       gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC       and with the beta chain to form the complete DNA polymerase III complex
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF83017.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE003849; AAF83017.1; ALT_INIT; Genomic_DNA.
DR   PIR; C82834; C82834.
DR   RefSeq; WP_010892745.1; NC_002488.3.
DR   AlphaFoldDB; Q9PGU4; -.
DR   SMR; Q9PGU4; -.
DR   STRING; 160492.XF_0204; -.
DR   EnsemblBacteria; AAF83017; AAF83017; XF_0204.
DR   KEGG; xfa:XF_0204; -.
DR   PATRIC; fig|160492.11.peg.216; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_0_0_6; -.
DR   OMA; NECRRMG; -.
DR   Proteomes; UP000000812; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..1193
FT                   /note="DNA polymerase III subunit alpha"
FT                   /id="PRO_0000103359"
SQ   SEQUENCE   1193 AA;  133503 MW;  B1E8D6CFF55C6765 CRC64;
     MSTSSFVHLH IHTEFSLADS TIRVPEKPEQ AHPKKAKQAN LLSRAVELGL PALAVTDLNN
     LFALIKFYKA AETVGIKPIS GADLLIAEPE HTPWGMTLLC RDHAGYLNLS QLISRGWLEG
     HRPEGGVAVH PDWVRDHHKN LFALIGRHSL AGQLFAKGRA DLAEQQLADW QHVFGNGLHL
     ELTRTGRDGE EPFNQFALQV AGIRGIPVIA SNDVRFLVPS DFLAHEARVC IASGRMLDDP
     KRPRTYSEQQ YLRSSEEMAA LFTDIPDALD NTRTLAQRCN IEMRLGTYFL PNYPVPNDET
     LDSWIRKQSH EGLEARLLKH PLAPGQTRED YVTRLEFELD TIIKMGFSGY FLIVADFIQW
     GKQQGIPIGP GRGSGAGSLV AWTLLITDLD PLPYNLLFER FLNPERVSMP DFDIDFCMER
     RDEVISYVAR KYGRERVSQI ITYGTMAAKA VVRDVGRVLG FPYGLVDSIA KLIPNTLGIT
     LKDAMGEGET NDNASAELIQ RYQAEEDVQE LLNLARQLED LTRNAGKHAG GVVIAPNPLT
     EFCPLFAEHD ENGRGKNPVT QFDKNDVEEV GLVKFDFLGL RTLTIIDWAV KAINKRHARA
     CIDPVDITAL PLDDIPTYKD IFASGNTSAV FQFESSGMRR LLKDARPDRF EDLIALVSLY
     RPGPMDLIPE FTARKHGVQE TIYPDPRTKN ILKDTYGIMV YQEQVMQMAQ IVGGYSLGSA
     DLLRRAMGKK VPAEMAKHRE IFREGAAKGG MDAVKADEIF DLMEKFAGYG FNKSHAAAYA
     LVSYQTAWLK RHYPAEFMAA TLSSDMDNTD KVVGFLDEAR NLNLKVLRPN INHSVYMFEA
     THADTIQYGL GAIKGVGQSV CEAIVKERLH YGPYTSLLNF CTRVASAKLN RRALEAMIHA
     GALDELGKNR ASVMLQLPEV IKATEQMSRE RESGQNPLFG NADPSTPAIQ LDLPECEEWP
     LTRMLNGERE TLGLYFSGHP FDPYRKQVKE LVGCDLNTSA LERILGSQQR GNGEKRTWHP
     EVNTILAGLV VSVRRKGDSQ VFVQLEDGRG RIECSAFSDA LAEFGHLLTR DRILIVKGGL
     REDEFNDGYS LRIRQCWDYT QLCTDYAQRL LLRVDLRTSD AWERIDAILA RYRPGNTPLR
     LDLLLNSTHG PVAGTLDLSG AQSVRIEQSL LDKLQKDPAV STLKVKYTPP WVQ