ID   DPO3A_XYLFT             Reviewed;        1193 AA.
AC   Q87EY0;
DT   16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=DNA polymerase III subunit alpha;
DE            EC=2.7.7.7;
GN   Name=dnaE; OrderedLocusNames=PD_0165;
OS   Xylella fastidiosa (strain Temecula1 / ATCC 700964).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xylella.
OX   NCBI_TaxID=183190;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Temecula1 / ATCC 700964;
RX   PubMed=12533478; DOI=10.1128/jb.185.3.1018-1026.2003;
RA   Van Sluys M.A., de Oliveira M.C., Monteiro-Vitorello C.B., Miyaki C.Y.,
RA   Furlan L.R., Camargo L.E.A., da Silva A.C.R., Moon D.H., Takita M.A.,
RA   Lemos E.G.M., Machado M.A., Ferro M.I.T., da Silva F.R., Goldman M.H.S.,
RA   Goldman G.H., Lemos M.V.F., El-Dorry H., Tsai S.M., Carrer H.,
RA   Carraro D.M., de Oliveira R.C., Nunes L.R., Siqueira W.J., Coutinho L.L.,
RA   Kimura E.T., Ferro E.S., Harakava R., Kuramae E.E., Marino C.L.,
RA   Giglioti E., Abreu I.L., Alves L.M.C., do Amaral A.M., Baia G.S.,
RA   Blanco S.R., Brito M.S., Cannavan F.S., Celestino A.V., da Cunha A.F.,
RA   Fenille R.C., Ferro J.A., Formighieri E.F., Kishi L.T., Leoni S.G.,
RA   Oliveira A.R., Rosa V.E. Jr., Sassaki F.T., Sena J.A.D., de Souza A.A.,
RA   Truffi D., Tsukumo F., Yanai G.M., Zaros L.G., Civerolo E.L.,
RA   Simpson A.J.G., Almeida N.F. Jr., Setubal J.C., Kitajima J.P.;
RT   "Comparative analyses of the complete genome sequences of Pierce's disease
RT   and citrus variegated chlorosis strains of Xylella fastidiosa.";
RL   J. Bacteriol. 185:1018-1026(2003).
CC   -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC       responsible for most of the replicative synthesis in bacteria. This DNA
CC       polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC       is the DNA polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC       and theta chains) that associates with a tau subunit. This core
CC       dimerizes to form the PolIII' complex. PolIII' associates with the
CC       gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC       and with the beta chain to form the complete DNA polymerase III complex
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE009442; AAO28059.1; -; Genomic_DNA.
DR   RefSeq; WP_004572983.1; NC_004556.1.
DR   AlphaFoldDB; Q87EY0; -.
DR   SMR; Q87EY0; -.
DR   PRIDE; Q87EY0; -.
DR   EnsemblBacteria; AAO28059; AAO28059; PD_0165.
DR   GeneID; 58015722; -.
DR   KEGG; xft:PD_0165; -.
DR   HOGENOM; CLU_001600_0_0_6; -.
DR   OMA; NECRRMG; -.
DR   Proteomes; UP000002516; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1193
FT                   /note="DNA polymerase III subunit alpha"
FT                   /id="PRO_0000103360"
SQ   SEQUENCE   1193 AA;  133311 MW;  AE3B76C6C861C978 CRC64;
     MSTSSFVHLH IHTEFSLADS TIRVPEKPEQ AHPKKAKQAN LLSRAVELGL PALAVTDLNN
     LFALIKFYKA AETVGIKPIS GADLLIAEPE HPPWGMTLLC RDHAGYLNLS QLISRGWLEG
     HRPEGGVAVH PDWVRDHHKN LFALIGRHSL AGQLLAKGRA DLAEQQLADW QHVFGDGLHL
     ELTRTGRDGE EPFNQFALQV AGIRGIPVIA SNDVRFLVQS DFLAHEARVC IASGRMLDDP
     KRPRTYSEQQ YLRSSEEMAA LFADIPDALD NTRTLAQRCN IEMRLGTYFL PNYPVPNDET
     LDSWIRKQSH EGLEARLLKH PLAPGQTRED YVTRLEFELD TIIKMGFSGY FLIVADFIQW
     GKQQGIPIGP GRGSGAGSLV AWALLITDLD PLPYNLLFER FLNPERVSMP DFDIDFCMER
     RDEVISYVAR KYGRERVSQI ITYGTMAAKA VVRDVGRVLG FPYGLVDSVA KLIPSTLGIT
     LKDAMGEGET NDNASAELIQ RYQAEEDVQE LLNLARQLED LTRNAGKHAG GVVIAPNPLT
     EFCPLFAEHD ENGRGKNPVT QFDKNDVEEV GLVKFDFLGL RTLTIIDWAV KAINKRHARA
     CIDPVDITAL PLDDIPTYKD VFASGNTSAV FQFESSGMRR LLKDARPDRF EDLIALVSLY
     RPGPMDLIPE FTARKHGVQE TIYPDPRTKN ILKDTYGIMV YQEQVMQMAQ IVGGYSLGSA
     DLLRRAMGKK VPAEMAKHRE IFREGAAKGG MDAVKADEIF DLMEKFAGYG FNKSHAAAYA
     LVSYQTAWLK RHYPAEFMAA TLSSDMDNTD KVVGFLDEAR NLNLKVLRPN INHSAYMFEA
     THADTIQYGL GAIKGVGQSV CEAIVKERLH YGPYTSLLDF CTRVTSAKLN RRALEAMIHA
     GALDELGKNR ASVMLQLPEV IKATEQMSRE RESGQNSLFG NADPGTPVIQ LDLPECEEWP
     LTRMLNGERE TLGLYFSGHP FDPYRKQVKE LVGCDLNTSA LERILGSQQR GNGEKRTWQP
     EVNTILAGLV VSVRRKGDSQ VFVQLEDGRG RIECSAFSDA LAEFGHLLTR DRILIVKGGL
     REDEFNGGYS LRIRQCWDYA QLCTDYAQRL LLRVDLRTSH AWERIDAILA RYRPGNTPLR
     LDLLLNSTHG PVAGTLDLSG GQSVRIEQSL LDKLQKDPAV SKLKVKYTPP WVQ