DPO3B_CAUVC
ID DPO3B_CAUVC Reviewed; 372 AA.
AC P0CAU5; P48198; Q45999;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Beta sliding clamp;
DE Short=Beta clamp;
DE Short=Sliding clamp;
DE AltName: Full=Beta-clamp processivity factor;
DE AltName: Full=DNA polymerase III beta sliding clamp subunit;
DE AltName: Full=DNA polymerase III subunit beta;
GN Name=dnaN; OrderedLocusNames=CC_0156;
OS Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter.
OX NCBI_TaxID=190650;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=11259647; DOI=10.1073/pnas.061029298;
RA Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E.,
RA Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R.,
RA Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B.,
RA DeBoy R.T., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F.,
RA Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R.,
RA Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O.,
RA Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M.;
RT "Complete genome sequence of Caulobacter crescentus.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001).
CC -!- FUNCTION: Confers DNA tethering and processivity to DNA polymerases and
CC other proteins. Acts as a clamp, forming a ring around DNA (a reaction
CC catalyzed by the clamp-loading complex) which diffuses in an ATP-
CC independent manner freely and bidirectionally along dsDNA. Initially
CC characterized for its ability to contact the catalytic subunit of DNA
CC polymerase III (Pol III), a complex, multichain enzyme responsible for
CC most of the replicative synthesis in bacteria; Pol III exhibits 3'-5'
CC exonuclease proofreading activity. The beta chain is required for
CC initiation of replication as well as for processivity of DNA
CC replication. {ECO:0000250|UniProtKB:P0A988}.
CC -!- SUBUNIT: Forms a ring-shaped head-to-tail homodimer around DNA which
CC binds and tethers DNA polymerases and other proteins to the DNA. The
CC DNA replisome complex has a single clamp-loading complex (3 tau and 1
CC each of delta, delta', psi and chi subunits) which binds 3 Pol III
CC cores (1 core on the leading strand and 2 on the lagging strand) each
CC with a beta sliding clamp dimer. Additional proteins in the replisome
CC are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA
CC primase. {ECO:0000250|UniProtKB:P0A988}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P0A988}.
CC -!- SIMILARITY: Belongs to the beta sliding clamp family. {ECO:0000305}.
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DR EMBL; AE005673; AAK22143.1; -; Genomic_DNA.
DR PIR; C87268; C87268.
DR RefSeq; NP_418975.1; NC_002696.2.
DR RefSeq; WP_010918045.1; NC_002696.2.
DR PDB; 5WCE; X-ray; 1.90 A; A/B=1-372.
DR PDB; 6IZO; X-ray; 1.94 A; A/B=1-372.
DR PDB; 6JIR; X-ray; 1.95 A; A/B=1-372.
DR PDBsum; 5WCE; -.
DR PDBsum; 6IZO; -.
DR PDBsum; 6JIR; -.
DR AlphaFoldDB; P0CAU5; -.
DR SMR; P0CAU5; -.
DR STRING; 190650.CC_0156; -.
DR EnsemblBacteria; AAK22143; AAK22143; CC_0156.
DR KEGG; ccr:CC_0156; -.
DR PATRIC; fig|190650.5.peg.152; -.
DR eggNOG; COG0592; Bacteria.
DR HOGENOM; CLU_038149_4_2_5; -.
DR OMA; YLIMPVR; -.
DR BioCyc; CAULO:CC0156-MON; -.
DR Proteomes; UP000001816; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009360; C:DNA polymerase III complex; IEA:InterPro.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd00140; beta_clamp; 1.
DR InterPro; IPR001001; DNA_polIII_beta.
DR InterPro; IPR022635; DNA_polIII_beta_C.
DR InterPro; IPR022637; DNA_polIII_beta_cen.
DR InterPro; IPR022634; DNA_polIII_beta_N.
DR PANTHER; PTHR30478; PTHR30478; 1.
DR Pfam; PF00712; DNA_pol3_beta; 1.
DR Pfam; PF02767; DNA_pol3_beta_2; 1.
DR Pfam; PF02768; DNA_pol3_beta_3; 1.
DR PIRSF; PIRSF000804; DNA_pol_III_b; 1.
DR SMART; SM00480; POL3Bc; 1.
DR TIGRFAMs; TIGR00663; dnan; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA replication; DNA-binding;
KW DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..372
FT /note="Beta sliding clamp"
FT /id="PRO_0000105430"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:5WCE"
FT HELIX 7..18
FT /evidence="ECO:0007829|PDB:5WCE"
FT HELIX 28..31
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 32..38
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 41..47
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 49..58
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 60..63
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 65..70
FT /evidence="ECO:0007829|PDB:5WCE"
FT HELIX 71..80
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 86..90
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 93..95
FT /evidence="ECO:0007829|PDB:6JIR"
FT STRAND 96..102
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:5WCE"
FT HELIX 114..116
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:5WCE"
FT HELIX 133..141
FT /evidence="ECO:0007829|PDB:5WCE"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 152..154
FT /evidence="ECO:0007829|PDB:6IZO"
FT STRAND 157..166
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 169..177
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 179..188
FT /evidence="ECO:0007829|PDB:5WCE"
FT TURN 191..195
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 199..202
FT /evidence="ECO:0007829|PDB:5WCE"
FT HELIX 203..213
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 218..224
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 226..233
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 236..241
FT /evidence="ECO:0007829|PDB:5WCE"
FT HELIX 250..252
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 260..265
FT /evidence="ECO:0007829|PDB:5WCE"
FT HELIX 266..277
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 282..284
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 286..292
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 295..301
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 303..305
FT /evidence="ECO:0007829|PDB:6JIR"
FT STRAND 307..313
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 321..326
FT /evidence="ECO:0007829|PDB:5WCE"
FT HELIX 327..336
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 339..346
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 353..357
FT /evidence="ECO:0007829|PDB:5WCE"
FT STRAND 360..367
FT /evidence="ECO:0007829|PDB:5WCE"
SQ SEQUENCE 372 AA; 40425 MW; B4B234778AD89BE4 CRC64;
MKLTIERAAL LKALGHVQSV VERRNTIPIL SNILLSAEGD RLSFSATDLD MEIIDEGFAQ
IDVPGQITAP AHTLYEIVRK LPDGADVSLS FSGDDPRLVI QAGRSRFNLP VLPAGDFPVM
SSDGLSSRIA VDTNELIRLI DKTRFAISTE ETRYYLNGLY VHTVNEGGET KLRAVATDGH
RLALAEMPAP EGAVGIPGVI VPRKTIAEAR RLMESAGETV DLQVSPQKVR FEFGAAALTS
KVIDGAFPDY MRVIPRDNAK ILTLDNDLFA KAVDRVATIS AEKSRSVKLA VEPGRITLTV
RNMEAGQAVE EVEVDYDGEP FEIGFNARYL LDVCGQIAGP QAEFRFADPA SPTLVVDPVD
PGVKYVLMPL RV
