ID   DPO3B_MYCCT             Reviewed;         375 AA.
AC   P24117; Q2STB1;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Beta sliding clamp;
DE            Short=Beta clamp;
DE            Short=Sliding clamp;
DE   AltName: Full=Beta-clamp processivity factor;
DE   AltName: Full=DNA polymerase III beta sliding clamp subunit;
DE   AltName: Full=DNA polymerase III subunit beta;
GN   Name=dnaN; OrderedLocusNames=MCAP_0002;
OS   Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343
OS   / NCTC 10154).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=340047;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=California kid / ATCC 27343 / NCTC 10154;
RA   Glass J.I., Lartigue C., Pfannkoch C., Baden-Tillson H., Smith H.O.,
RA   Venter J.C., Roske K., Wise K.S., Calcutt M.J., Nelson W.C., Nierman W.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-210.
RX   PubMed=1544573; DOI=10.1016/0378-1119(92)90439-v;
RA   Fujita M.Q., Yoshikawa H., Ogasawara N.;
RT   "Structure of the dnaA and DnaA-box region in the Mycoplasma capricolum
RT   chromosome: conservation and variations in the course of evolution.";
RL   Gene 110:17-23(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-375.
RX   PubMed=8233831; DOI=10.1093/nar/21.20.4816;
RA   Miyata M., Sano K., Okada R., Fukumura T.;
RT   "Mapping of replication initiation site in Mycoplasma capricolum genome by
RT   two-dimensional gel-electrophoretic analysis.";
RL   Nucleic Acids Res. 21:4816-4823(1993).
CC   -!- FUNCTION: Confers DNA tethering and processivity to DNA polymerases and
CC       other proteins. Acts as a clamp, forming a ring around DNA (a reaction
CC       catalyzed by the clamp-loading complex) which diffuses in an ATP-
CC       independent manner freely and bidirectionally along dsDNA. Initially
CC       characterized for its ability to contact the catalytic subunit of DNA
CC       polymerase III (Pol III), a complex, multichain enzyme responsible for
CC       most of the replicative synthesis in bacteria; Pol III exhibits 3'-5'
CC       exonuclease proofreading activity. The beta chain is required for
CC       initiation of replication as well as for processivity of DNA
CC       replication. {ECO:0000250|UniProtKB:P0A988}.
CC   -!- SUBUNIT: Forms a ring-shaped head-to-tail homodimer around DNA which
CC       binds and tethers DNA polymerases and other proteins to the DNA. The
CC       DNA replisome complex has a single clamp-loading complex (3 tau and 1
CC       each of delta, delta', psi and chi subunits) which binds 3 Pol III
CC       cores (1 core on the leading strand and 2 on the lagging strand) each
CC       with a beta sliding clamp dimer. Additional proteins in the replisome
CC       are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA
CC       primase. {ECO:0000250|UniProtKB:P0A988}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P0A988}.
CC   -!- SIMILARITY: Belongs to the beta sliding clamp family. {ECO:0000305}.
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DR   EMBL; CP000123; ABC01687.1; -; Genomic_DNA.
DR   EMBL; D90426; BAA14416.1; -; Genomic_DNA.
DR   EMBL; D14983; BAA03628.1; -; Genomic_DNA.
DR   PIR; JN0277; JN0277.
DR   PIR; S42119; S42119.
DR   RefSeq; WP_011386907.1; NC_007633.1.
DR   AlphaFoldDB; P24117; -.
DR   SMR; P24117; -.
DR   EnsemblBacteria; ABC01687; ABC01687; MCAP_0002.
DR   GeneID; 23779041; -.
DR   KEGG; mcp:MCAP_0002; -.
DR   HOGENOM; CLU_038149_2_0_14; -.
DR   OMA; YLIMPVR; -.
DR   OrthoDB; 1040142at2; -.
DR   PhylomeDB; P24117; -.
DR   Proteomes; UP000001928; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009360; C:DNA polymerase III complex; IEA:InterPro.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd00140; beta_clamp; 1.
DR   InterPro; IPR001001; DNA_polIII_beta.
DR   InterPro; IPR022635; DNA_polIII_beta_C.
DR   InterPro; IPR022637; DNA_polIII_beta_cen.
DR   InterPro; IPR022634; DNA_polIII_beta_N.
DR   PANTHER; PTHR30478; PTHR30478; 1.
DR   Pfam; PF00712; DNA_pol3_beta; 1.
DR   Pfam; PF02767; DNA_pol3_beta_2; 1.
DR   Pfam; PF02768; DNA_pol3_beta_3; 1.
DR   SMART; SM00480; POL3Bc; 1.
DR   TIGRFAMs; TIGR00663; dnan; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-binding; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Transferase.
FT   CHAIN           1..375
FT                   /note="Beta sliding clamp"
FT                   /id="PRO_0000105444"
SQ   SEQUENCE   375 AA;  42716 MW;  7F02C40C47BD4336 CRC64;
     MNFSINRIVL LDNLSKAAKV IDYKNVNPSL SGIYLNVLND QVNVITTSGI LSFKSILNNQ
     NSDLEVKQEG KVLLKPKYVL EMLRRLDDEF VVFSMVEDNE LIIKTNNSDF SIGVLNSEDY
     PLIGFREKGI EFNLNPKEVK KTIYQVFVSM NENNKKLILT GLNLKLNNNQ AIFSTTDSFR
     ISQKILEIQS DNNEDIDITI PFKTALELPK LLDNAENLKI IIVEGYITFI IDNVIFQSNL
     IDGKFPNVQI AFPTKFETII TVKQKSILKV LSRFDLVADD GLPAIVNIKV NEDKIEFKSF
     ISEVGKYEED FDDFVIEGNK SLSISFNTRF LIDAIKTLSE DRIELKLINS TKPIVINNVY
     DEHLKQVILP TFLSN